Adiponectin antibody | Adn36
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E ResetMouse anti Human Adiponectin
- Product Type
- Monoclonal Antibody
- Clone
- Adn36
- Isotype
- IgG2a
- Specificity
- Adiponectin
Antibody Quality and Performance Guarantee
| Mouse anti Human Adiponectin antibody, clone Adn36 recognizes human adiponectin, also known as Acrp30, 30 kDa adipocyte complement-related protein, Adipocyte, C1q and collagen domain-containing protein or Gelatin-binding protein. Adiponectin is a 244 amino acid major adipokine secreted into the bloodstream from adipose tissue to modulate metabolism, including glucose regulation and fatty acid catabolism (UniProt: Q15848). Adiponectin circulates as three distinct oligomeric forms, trimers (LMW), hexamers (MMW) and dodecamers-octodecamers (HMW) with distinct functions in various target tissues (Wang et al. 2008). Adiponectin is involved in regulation of circulating glucose levels and in regulation of insulin sensitivity. Concentrations of adiponectin are reduced in obesity associated metabolic and vascular disorders (Fasshauer et al. 2004) in particular lower circulating levels of HMW adiponectin correlates with the appearance of type II diabetes and acts as a predictor for this condition (Zhu et al. 2010). Mouse anti human adiponectin antibody, clone Adn36 has been used successfully in the development of a sensitive sandwich ELISA in conjunction with Mouse anti Human adiponectin antibody, clone 5H7 (MCA2695) as a detection reagent for the measurement of circulating levels of adiponectin in human serum. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Recombinant human adiponectin purified from E.coli.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Adiponectin antibody
-
Lihn, A.S. et al. (2005) Adiponectin: action, regulation and association to insulin sensitivity.
Obes Rev. 6 (1): 13-21. -
Trayhurn, P. & Wood, I.S. (2005) Signalling role of adipose tissue: adipokines and inflammation in obesity.
Biochem Soc Trans. 33 (Pt 5): 1078-81.
- Synonyms
- ADIPOQ
- RRID
- AB_2221632
- UniProt
- Q15848
- Entrez Gene
- ADIPOQ
- GO Terms
- GO:0005125 cytokine activity
- GO:0005179 hormone activity
- GO:0005615 extracellular space
- GO:0006006 glucose metabolic process
- GO:0006091 generation of precursor metabolites and energy
- GO:0006635 fatty acid beta-oxidation
- GO:0009749 response to glucose stimulus
- GO:0010739 positive regulation of protein kinase A signaling cascade
- GO:0010745 negative regulation of macrophage derived foam cell differentiation
- View More GO Terms
- GO:0010804 negative regulation of tumor necrosis factor-mediated signaling pathway
- GO:0010875 positive regulation of cholesterol efflux
- GO:0046326 positive regulation of glucose import
- GO:0030853 negative regulation of granulocyte differentiation
- GO:0032720 negative regulation of tumor necrosis factor production
- GO:0033034 positive regulation of myeloid cell apoptosis
- GO:0034115 negative regulation of heterotypic cell-cell adhesion
- GO:0034383 low-density lipoprotein particle clearance
- GO:0034612 response to tumor necrosis factor
- GO:0042593 glucose homeostasis
- GO:0042803 protein homodimerization activity
- GO:0043124 negative regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0043499 eukaryotic cell surface binding
- GO:0045599 negative regulation of fat cell differentiation
- GO:0045650 negative regulation of macrophage differentiation
- GO:0045715 negative regulation of low-density lipoprotein particle receptor biosynthetic process
- GO:0045721 negative regulation of gluconeogenesis
- GO:0045923 positive regulation of fatty acid metabolic process
- GO:0050728 negative regulation of inflammatory response
- GO:0050765 negative regulation of phagocytosis
MCA4653
147498
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