Adiponectin antibody | Adn27

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Mouse anti Human Adiponectin

Product Type
Monoclonal Antibody
Clone
Adn27
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA4652 E 0.2 mg
Mouse anti Human Adiponectin antibody, clone Adn27 recognizes human adiponectin, also known as Acrp30, 30 kDa adipocyte complement-related protein, Adipocyte, C1q and collagen domain-containing protei or Gelatin-binding protein. Adiponectin is a 244 amino acid major adipokine secreted into the bloodstream from adipose tissue to modulate metabolism, including glucose regulation and fatty acid catabolism.

Unlike most other adipokines, adiponectin is secreted exclusively by differentiating adipocytes at reduced levels during obesity. The serum level of adiponectin is inversely correlated with BMI (body mass index) of an individual, and has an anti-inflammatory action, playing an important role in type II diabetes (insulin sensitivity) and atherosclerosis.

Mouse anti human adiponectin antibody, clone Adn27 has been successfully used as a capture reagent with mouse anti human adionectin antibody, Mouse anti Human adiponectin antibody, clone 5H7 (MCA2695) as a detection reagent in a sensitive Sandwich ELISA for the measurement of adiponectin in human plasma.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human adiponectin purified from E.coli.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q15848 Related reagents
Entrez Gene
ADIPOQ Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005179 hormone activity
GO:0005615 extracellular space
GO:0006006 glucose metabolic process
GO:0006091 generation of precursor metabolites and energy
GO:0006635 fatty acid beta-oxidation
GO:0009749 response to glucose stimulus
GO:0010739 positive regulation of protein kinase A signaling cascade
GO:0010745 negative regulation of macrophage derived foam cell differentiation
GO:0010804 negative regulation of tumor necrosis factor-mediated signaling pathway
GO:0010875 positive regulation of cholesterol efflux
GO:0046326 positive regulation of glucose import
GO:0030853 negative regulation of granulocyte differentiation
GO:0032720 negative regulation of tumor necrosis factor production
GO:0033034 positive regulation of myeloid cell apoptosis
GO:0034115 negative regulation of heterotypic cell-cell adhesion
GO:0034383 low-density lipoprotein particle clearance
GO:0034612 response to tumor necrosis factor
GO:0042593 glucose homeostasis
GO:0042803 protein homodimerization activity
GO:0043124 negative regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043499 eukaryotic cell surface binding
GO:0045599 negative regulation of fat cell differentiation
GO:0045650 negative regulation of macrophage differentiation
GO:0045715 negative regulation of low-density lipoprotein particle receptor biosynthetic process
GO:0045721 negative regulation of gluconeogenesis
GO:0045923 positive regulation of fatty acid metabolic process
GO:0050728 negative regulation of inflammatory response
GO:0050765 negative regulation of phagocytosis
Regulatory
For research purposes only

Applications of Adiponectin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for Adiponectin antibody

  1. Lihn, A.S. et al. (2005) Adiponectin: action, regulation and association to insulin sensitivity.
    Obes Rev. 6 (1): 13-21.
  2. Trayhurn, P. & Wood, I.S. (2005) Signalling role of adipose tissue: adipokines and inflammation in obesity.
    Biochem Soc Trans. 33 (Pt 5): 1078-81.