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Adiponectin antibody | 5H7

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Anti Human Adiponectin Antibody, clone 5H7 thumbnail image 1 Anti Human Adiponectin Antibody, clone 5H7 thumbnail image 2
Anti Human Adiponectin Antibody, clone 5H7 gallery image 1
Sandwich ELISA analysis of human adiponectin binding using Mouse anti Human adiponectin (MCA4652) as a capture reagent and biotinylated Mouse anti Human adiponectin (MCA2695) as a detection reagent with recombinant human adiponectin (PHP196) as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A plasma sample diluted 1:4 (green) is shown.
Anti Human Adiponectin Antibody, clone 5H7 gallery image 2
Sandwich ELISA analysis of human adiponectin binding using Mouse anti Human adiponectin (MCA4653) as a capture reagent and biotinylated Mouse anti Human adiponectin (MCA2695) as a detection reagent with recombinant human adiponectin (PHP196) as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A plasma sample diluted 1:4 (green) is shown.

Mouse anti Human Adiponectin

Product Type
Monoclonal Antibody
Clone
5H7
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2695
Datasheet
E WB
SDS
0.1 mg loader

Mouse anti Human adiponectin, clone 5H7 recognizes the collagen-like domain of human adiponectin, also known as Acrp30, Gelatin-binding protein, Adipose most abundant gene transcript 1 protein, Adipocyte complement-related 30 kDa protein or apM-1. Adiponectin a 244 amino acid ~30 kDa major adipokine secreted into the bloodstream from adipose tissue into the circulation where it exists as three distinct, low, medium and high molecular weight oligomeric forms (UniProt: Q15848).

Circulating adiponectin levels are correlated with insulin sensitivity and decreased levels parallel the change in insulin sensitivity during the progression to type II diabetes (Heilbronn et al. 2011). Mutations in the adiponectin gene can also lead to adiponectin deficiency characterized by obesity, diabetes, high blood pressure and circulating cholesterol (Takahashi et al. 2000).

Mouse anti Human adiponectin, clone 5H7 had been used successfully as a detection reagent in the development of sensitive sandwich ELISA's in conjunction with Mouse anti adiponectin clones Mouse anti Human adiponectin, clone Adn27 (MCA4652) or Mouse anti Human adiponectin antibody, clone Adn36 (MCA4653) as capture reagents.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3), 10% Glycerol
Immunogen
Recombinant human adiponectin (amino acids 15-244) purified from Escherichia coli .
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q15848
Entrez Gene
ADIPOQ
GO Terms
GO:0005125 cytokine activity
GO:0005179 hormone activity
GO:0005615 extracellular space
GO:0006006 glucose metabolic process
GO:0006091 generation of precursor metabolites and energy
GO:0006635 fatty acid beta-oxidation
GO:0009749 response to glucose stimulus
GO:0010739 positive regulation of protein kinase A signaling cascade
GO:0010745 negative regulation of macrophage derived foam cell differentiation
GO:0010804 negative regulation of tumor necrosis factor-mediated signaling pathway
GO:0010875 positive regulation of cholesterol efflux
GO:0046326 positive regulation of glucose import
GO:0030853 negative regulation of granulocyte differentiation
GO:0032720 negative regulation of tumor necrosis factor production
GO:0033034 positive regulation of myeloid cell apoptosis
GO:0034115 negative regulation of heterotypic cell-cell adhesion
GO:0034383 low-density lipoprotein particle clearance
GO:0034612 response to tumor necrosis factor
GO:0042593 glucose homeostasis
GO:0042803 protein homodimerization activity
GO:0043124 negative regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043499 eukaryotic cell surface binding
GO:0045599 negative regulation of fat cell differentiation
GO:0045650 negative regulation of macrophage differentiation
GO:0045715 negative regulation of low-density lipoprotein particle receptor biosynthetic process
GO:0045721 negative regulation of gluconeogenesis
GO:0045923 positive regulation of fatty acid metabolic process
GO:0050728 negative regulation of inflammatory response
GO:0050765 negative regulation of phagocytosis
Regulatory
For research purposes only

Applications of Adiponectin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1/1000 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA2695 detects a band of approximately 64kDa in mouse liver cell lysates.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

Further Reading

  1. Lihn, A.S. et al. (2005) Adiponectin: action, regulation and association to insulin sensitivity.
    Obes Rev. 6 (1): 13-21.
  2. Trayhurn, P. & Wood, I.S. (2005) Signalling role of adipose tissue: adipokines and inflammation in obesity.
    Biochem Soc Trans. 33 (Pt 5): 1078-81.

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