Adiponectin antibody | 5H7

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Anti Human Adiponectin Antibody, clone 5H7 thumbnail image 1

Anti Human Adiponectin Antibody, clone 5H7 thumbnail image 2

Anti Human Adiponectin Antibody, clone 5H7 gallery image 1 — Sandwich ELISA analysis of human adiponectin binding using Mouse anti Human adiponectin (MCA4652) as a capture reagent and biotinylated Mouse anti Human adiponectin (**MCA2695**) as a detection reagent with recombinant human adiponectin (PHP196) as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A plasma sample diluted 1:4 (green) is shown.

Anti Human Adiponectin Antibody, clone 5H7 gallery image 2 — Sandwich ELISA analysis of human adiponectin binding using Mouse anti Human adiponectin (MCA4653) as a capture reagent and biotinylated Mouse anti Human adiponectin (**MCA2695**) as a detection reagent with recombinant human adiponectin (PHP196) as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A plasma sample diluted 1:4 (green) is shown.

Mouse anti Human Adiponectin

Product Type: Monoclonal Antibody
Clone: 5H7
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2695	E WB			0.1 mg

Mouse anti Human adiponectin, clone 5H7 recognizes the collagen-like domain of human adiponectin, also known as Acrp30, Gelatin-binding protein, Adipose most abundant gene transcript 1 protein, Adipocyte complement-related 30 kDa protein or apM-1. Adiponectin a 244 amino acid ~30 kDa major adipokine secreted into the bloodstream from adipose tissue into the circulation where it exists as three distinct, low, medium and high molecular weight oligomeric forms (UniProt: Q15848).

Circulating adiponectin levels are correlated with insulin sensitivity and decreased levels parallel the change in insulin sensitivity during the progression to type II diabetes (Heilbronn et al. 2011). Mutations in the adiponectin gene can also lead to adiponectin deficiency characterized by obesity, diabetes, high blood pressure and circulating cholesterol (Takahashi et al. 2000).

Mouse anti Human adiponectin, clone 5H7 had been used successfully as a detection reagent in the development of sensitive sandwich ELISA's in conjunction with Mouse anti adiponectin clones Mouse anti Human adiponectin, clone Adn27 (MCA4652) or Mouse anti Human adiponectin antibody, clone Adn36 (MCA4653) as capture reagents.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.02% Sodium Azide (NaN₃), 10% Glycerol

Immunogen

Recombinant human adiponectin (amino acids 15-244) purified from Escherichia coli .

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Fusion Partners

Spleen cells from immunised Balb/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: Q15848 Related reagents
Entrez Gene: ADIPOQ Related reagents
GO Terms: GO:0005125 cytokine activity; GO:0005179 hormone activity; GO:0005615 extracellular space; GO:0006006 glucose metabolic process; GO:0006091 generation of precursor metabolites and energy; GO:0006635 fatty acid beta-oxidation; GO:0009749 response to glucose stimulus; GO:0010739 positive regulation of protein kinase A signaling cascade; GO:0010745 negative regulation of macrophage derived foam cell differentiation; GO:0010804 negative regulation of tumor necrosis factor-mediated signaling pathway; GO:0010875 positive regulation of cholesterol efflux; GO:0046326 positive regulation of glucose import; GO:0030853 negative regulation of granulocyte differentiation; GO:0032720 negative regulation of tumor necrosis factor production; GO:0033034 positive regulation of myeloid cell apoptosis; GO:0034115 negative regulation of heterotypic cell-cell adhesion; GO:0034383 low-density lipoprotein particle clearance; GO:0034612 response to tumor necrosis factor; GO:0042593 glucose homeostasis; GO:0042803 protein homodimerization activity; GO:0043124 negative regulation of I-kappaB kinase/NF-kappaB cascade; GO:0043499 eukaryotic cell surface binding; GO:0045599 negative regulation of fat cell differentiation; GO:0045650 negative regulation of macrophage differentiation; GO:0045715 negative regulation of low-density lipoprotein particle receptor biosynthetic process; GO:0045721 negative regulation of gluconeogenesis; GO:0045923 positive regulation of fatty acid metabolic process; GO:0050728 negative regulation of inflammatory response; GO:0050765 negative regulation of phagocytosis
Regulatory: For research purposes only

Applications of Adiponectin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Western Blotting		1/1000	1/2000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: MCA2695 detects a band of approximately 64kDa in mouse liver cell lysates.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F