ADAP antibody | AbD06348

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Human anti Human ADAP

Product Type
Monoclonal Antibody
Clone
AbD06348
Isotype
HuCAL Fab bivalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA092 E IP 0.1 mg
Human anti Human ADAP antibody, clone AbD06348 recognizes the human molecular adaptor protein adhesion- and degranulation-promoting (ADAP). Adaptor proteins are essential components of the T-cell receptor (TCR) signaling cascade, regulating gene transcription and cytoskeletal re-organisation.

ADAP is a 130 kDa molecule which interacts with several intermediates of the T-cell signaling cascade including SLP-76, FYN and VASP. The ADAP molecule is expressed on hematopoietic tissues and is reported to have multiple functions downstream of the TCR that promote optimal T lymphocyte response to antigen. In particular, ADAP is reported to function as a positive regulator of T-cell activation, LFA-1 integrin clustering following TCR ligation and T-cell adhesion.

Product Details

Target Species
Human
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This Fab fragment is dimerized via a helix-turn-helix motif. This antibody is supplied lyophilized.
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
SH3 domain of ADAP.
Approx. Protein Concentrations
Ig concentration 0.1 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution

More Information

UniProt
O15117 Related reagents
Entrez Gene
FYB Related reagents
GO Terms
GO:0005515 protein binding
GO:0005829 cytosol
GO:0005634 nucleus
GO:0006468 protein phosphorylation
GO:0006607 NLS-bearing substrate import into nucleus
GO:0006955 immune response
GO:0007243 intracellular protein kinase cascade
GO:0034329 cell junction assembly
GO:0050852 T cell receptor signaling pathway
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of ADAP antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Histidine Tag:Biotin MCA1396B 0.1 mg E WB
Mouse anti c-Myc:Alk.Phos. MCA2200A 0.1 mg C E P WB*
Mouse anti c-Myc:Biotin MCA2200B 0.1 mg C E P WB*
Mouse anti c-Myc:HRP MCA2200P 0.1 mg C E P WB*
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB