ADAP antibody | AbD06348
ADAP is a 130 kDa molecule which interacts with several intermediates of the T-cell signaling cascade including SLP-76, FYN and VASP. The ADAP molecule is expressed on hematopoietic tissues and is reported to have multiple functions downstream of the TCR that promote optimal T lymphocyte response to antigen. In particular, ADAP is reported to function as a positive regulator of T-cell activation, LFA-1 integrin clustering following TCR ligation and T-cell adhesion.
Product Details
- Target Species
- Human
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This Fab fragment is dimerized via a helix-turn-helix motif. This antibody is supplied lyophilized.
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- SH3 domain of ADAP.
- Approx. Protein Concentrations
- Ig concentration 0.1 mg/ml after reconstitution
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of reconstitution
More Information
- UniProt
- O15117 Related reagents
- Entrez Gene
- FYB Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0006468 protein phosphorylation
- GO:0006607 NLS-bearing substrate import into nucleus
- GO:0006955 immune response
- GO:0007243 intracellular protein kinase cascade
- GO:0034329 cell junction assembly
- GO:0050852 T cell receptor signaling pathway
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences. - Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
- Regulatory
- For research purposes only
Applications of ADAP antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Immunoprecipitation |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse anti Histidine Tag:Biotin | MCA1396B | E WB | 0.1 mg |
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Mouse anti c-Myc:Alk.Phos. | MCA2200A | C E P WB* | 0.1 mg | ||
Mouse anti c-Myc:Biotin | MCA2200B | C E P WB* | 0.1 mg |
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Mouse anti c-Myc:HRP | MCA2200P | C E P WB* | 0.1 mg |
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Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
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