ADAP antibody | AbD06348
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WB ResetHuman anti Human ADAP
- Product Type
- Monoclonal Antibody
- Clone
- AbD06348
- Isotype
- HuCAL Fab bivalent
- Specificity
- ADAP
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| Human anti Human ADAP antibody, clone AbD06348 recognizes the human molecular adaptor protein adhesion- and degranulation-promoting (ADAP). Adaptor proteins are essential components of the T-cell receptor (TCR) signaling cascade, regulating gene transcription and cytoskeletal re-organisation. ADAP is a 130 kDa molecule which interacts with several intermediates of the T-cell signaling cascade including SLP-76, FYN and VASP. The ADAP molecule is expressed on hematopoietic tissues and is reported to have multiple functions downstream of the TCR that promote optimal T lymphocyte response to antigen. In particular, ADAP is reported to function as a positive regulator of T-cell activation, LFA-1 integrin clustering following TCR ligation and T-cell adhesion. |
- Target Species
- Human
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This Fab fragment is dimerized via a helix-turn-helix motif. This antibody is supplied liquid.
- Preparation
- Metal chelate affinity chromatography.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.01% Thiomersal.
- Immunogen
- SH3 domain of ADAP.
- Approx. Protein Concentrations
- 0.5 mg/ml.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a trademark of EMD Biosciences.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
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- RRID
- AB_10841621
- UniProt
- O15117
- Entrez Gene
- FYB
- GO Terms
- GO:0005515 protein binding
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0006468 protein phosphorylation
- GO:0006607 NLS-bearing substrate import into nucleus
- GO:0006955 immune response
- GO:0007243 intracellular protein kinase cascade
- GO:0034329 cell junction assembly
- GO:0050852 T cell receptor signaling pathway
- View More GO Terms
- Licensed Use
- For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.
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