ADAP antibody | AbD06348

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Anti Human ADAP Antibody, clone AbD06348 thumbnail image 1

Anti Human ADAP Antibody, clone AbD06348 gallery image 1 — ADAP (shown in red) and it's direct interaction partner SKAP55 (shown in green) immunoprecipitated from Jurkat lysate using Human anti ADAP clone AbD06348 (**HCA092**). An irrelevant IgG was used as IP control and Jurkat lysate as Western blot control. This image was kindly provided by R. Lehmann, Leibniz-Institute for Molecular Pharmacology, Berlin, Germany

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Human anti Human ADAP

Product Type: Monoclonal Antibody
Clone: AbD06348
Isotype: HuCAL Fab bivalent
Specificity: ADAP

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Product Code Applications Pack Size List Price Your Price Qty

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HCA092 Datasheet Datasheet SDS SDS	E IP	0.1 mg		Log in	Get Quote
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Human anti Human ADAP antibody, clone AbD06348 recognizes the human molecular adaptor protein adhesion- and degranulation-promoting (ADAP). Adaptor proteins are essential components of the T-cell receptor (TCR) signaling cascade, regulating gene transcription and cytoskeletal re-organisation.

ADAP is a 130 kDa molecule which interacts with several intermediates of the T-cell signaling cascade including SLP-76, FYN and VASP. The ADAP molecule is expressed on hematopoietic tissues and is reported to have multiple functions downstream of the TCR that promote optimal T lymphocyte response to antigen. In particular, ADAP is reported to function as a positive regulator of T-cell activation, LFA-1 integrin clustering following TCR ligation and T-cell adhesion.

Target Species: Human
Product Form: A bivalent human recombinant Fab selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This Fab fragment is dimerized via a helix-turn-helix motif. This antibody is supplied liquid.
Preparation: Metal chelate affinity chromatography
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.01% Thiomersal
Immunogen: SH3 domain of ADAP.
Approx. Protein Concentrations: 0.5 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Immunoprecipitation

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice: Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.

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RRID: AB_10841621
UniProt: O15117
Entrez Gene: FYB
GO Terms: GO:0005515 protein binding; GO:0005829 cytosol; GO:0005634 nucleus; GO:0006468 protein phosphorylation; GO:0006607 NLS-bearing substrate import into nucleus; GO:0006955 immune response; GO:0007243 intracellular protein kinase cascade; GO:0034329 cell junction assembly; GO:0050852 T cell receptor signaling pathway; View More GO Terms
Licensed Use: For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.