ACTININ-1 antibody | rAB01-3G11

Mouse anti ACTININ-1

Product Type
PrecisionAb Monoclonal
Clone
rAB01-3G11
Isotype
IgG2b
Format
Purified
Specificity
ACTININ-1

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VMA00901
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Mouse anti actinin-1 antibody recognizes alpha-actinin-1, also known as ACTN1.

Actinin-1 is a cytoskeletal protein that binds actin in multiple cell types and cytoskeleton frameworks. Actinin-1 is located along the actin filaments and in adhesion sites in non-muscle cells, and at the Z-disk and analogous dense bodies in striated, cardiac and smooth muscle cells. The protein is important for organization of the cytoskeleton and for muscle contraction (Sjöblom et al. 2008), as well as cell adhesion and migration. Mutations in the gene encoding actinin-1 lead to the platelet disorder macrothrombocytopenia, associated with excessive bleeding (Murphy and Young 2015). In breast cancer patients, increased expression of actinin-1 has been identified and associates with poor prognosis in basal-like subtypes. It has been suggested as a potential prognostic biomarker in basal-like breast cancer (Kovac et al. 2018).
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti actinin-1 antibody detects a band of approximately 105 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Recombinant IgG prepared by affinity chromatography on Protein A from cell culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli--derived recombinant protein of amino acids 1-499 of human actinin-1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
As supplied, 12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20oC, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt
P12814
Entrez Gene
ACTN1
GO Terms
GO:0005509 calcium ion binding
GO:0002576 platelet degranulation
GO:0003779 actin binding
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0005178 integrin binding
GO:0005730 nucleolus
GO:0005925 focal adhesion
GO:0015629 actin cytoskeleton
GO:0042981 regulation of apoptosis
GO:0017166 vinculin binding
GO:0030018 Z disc
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0031143 pseudopodium
GO:0048041 focal adhesion assembly
GO:0051271 negative regulation of cellular component movement
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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