ACTININ-1 antibody | rAB01-3G11
Mouse anti actinin-1 antibody recognizes alpha-actinin-1, also known as ACTN1.
Actinin-1 is a cytoskeletal protein that binds actin in multiple cell types and cytoskeleton frameworks. Actinin-1 is located along the actin filaments and in adhesion sites in non-muscle cells, and at the Z-disk and analogous dense bodies in striated, cardiac and smooth muscle cells. The protein is important for organization of the cytoskeleton and for muscle contraction (Sjöblom et al. 2008), as well as cell adhesion and migration. Mutations in the gene encoding actinin-1 lead to the platelet disorder macrothrombocytopenia, associated with excessive bleeding (Murphy and Young 2015). In breast cancer patients, increased expression of actinin-1 has been identified and associates with poor prognosis in basal-like subtypes. It has been suggested as a potential prognostic biomarker in basal-like breast cancer (Kovac et al. 2018).
Actinin-1 is a cytoskeletal protein that binds actin in multiple cell types and cytoskeleton frameworks. Actinin-1 is located along the actin filaments and in adhesion sites in non-muscle cells, and at the Z-disk and analogous dense bodies in striated, cardiac and smooth muscle cells. The protein is important for organization of the cytoskeleton and for muscle contraction (Sjöblom et al. 2008), as well as cell adhesion and migration. Mutations in the gene encoding actinin-1 lead to the platelet disorder macrothrombocytopenia, associated with excessive bleeding (Murphy and Young 2015). In breast cancer patients, increased expression of actinin-1 has been identified and associates with poor prognosis in basal-like subtypes. It has been suggested as a potential prognostic biomarker in basal-like breast cancer (Kovac et al. 2018).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti actinin-1 antibody detects a band of approximately 105 kDa in HeLa cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Recombinant IgG prepared by affinity chromatography on Protein A from cell culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- E. coli--derived recombinant protein of amino acids 1-499 of human actinin-1
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Guarantee
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P12814
- Entrez Gene
- ACTN1
- GO Terms
- GO:0005509 calcium ion binding
- GO:0002576 platelet degranulation
- GO:0003779 actin binding
- GO:0005576 extracellular region
- GO:0005829 cytosol
- GO:0005178 integrin binding
- GO:0005730 nucleolus
- GO:0005925 focal adhesion
- GO:0015629 actin cytoskeleton
- GO:0042981 regulation of apoptosis
- GO:0017166 vinculin binding
- GO:0030018 Z disc
- GO:0030168 platelet activation
- GO:0031093 platelet alpha granule lumen
- GO:0031143 pseudopodium
- GO:0048041 focal adhesion assembly
- GO:0051271 negative regulation of cellular component movement
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of ACTININ-1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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