Actin Gamma antibody

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Mouse anti Actin Gamma

Actin gamma antibody recognizes cytoplasmic actin gamma, also known as gamma-actin or cytoplasmic 2. The antibody has been extensively validated for western blotting using whole cell lysates.

Actin Gamma Antibody with Control Lysate

Actin gamma antibody recognizes cytoplasmic actin gamma, also known as gamma-actin or cytoplasmic 2. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2b
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00049 WB 100 µl
VMA00049KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human actin gamma antibody recognizes cytoplasmic actin gamma, a 375 amino acid cytoskeletal protein also known as ACTG and gamma actin.

Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified (Vandekerckhove and Weber 1978). Mouse anti Human actin gamma antibody is highly specific for actin gamma and does not react with other actin isoforms.

Mouse anti Human actin gamma antibody recognizes actin gamma as a single band of ~42 kDa in multiple human cell line lysates by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti actin gamma antibody detects a band of approximately 42 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
African green monkey
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to the N-terminal nonapeptide of gamma-cytoplasmic actin coupled to KLH
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P63261 Related reagents
Entrez Gene
ACTG1 Related reagents
GO Terms
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0005200 structural constituent of cytoskeleton
GO:0005524 ATP binding
GO:0005856 cytoskeleton
GO:0006928 cellular component movement
GO:0007411 axon guidance
GO:0042802 identical protein binding
GO:0034329 cell junction assembly
GO:0034332 adherens junction organization
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Actin Gamma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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