Actin Gamma antibody | 2A3
Mouse anti Human actin gamma antibody recognizes cytoplasmic actin gamma, a 375 amino acid cytoskeletal protein also known as ACTG and gamma actin.
Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified (Vandekerckhove and Weber 1978). Mouse anti Human actin gamma antibody is highly specific for actin gamma and does not react with other actin isoforms.
Mouse anti Human actin gamma antibody recognizes actin gamma as a single band of ~42 kDa in multiple human cell line lysates by western blotting under reducing conditions.
Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified (Vandekerckhove and Weber 1978). Mouse anti Human actin gamma antibody is highly specific for actin gamma and does not react with other actin isoforms.
Mouse anti Human actin gamma antibody recognizes actin gamma as a single band of ~42 kDa in multiple human cell line lysates by western blotting under reducing conditions.
Product Details
- Target Species
- Human
- Western Blotting
- Anti actin gamma antibody detects a band of approximately 42 kDa in HeLa cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity African green monkey Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to the N-terminal nonapeptide of gamma-cytoplasmic actin coupled to KLH
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P63261
- Entrez Gene
- ACTG1
- GO Terms
- GO:0007596 blood coagulation
- GO:0005829 cytosol
- GO:0005200 structural constituent of cytoskeleton
- GO:0005524 ATP binding
- GO:0005856 cytoskeleton
- GO:0006928 cellular component movement
- GO:0007411 axon guidance
- GO:0042802 identical protein binding
- GO:0034329 cell junction assembly
- GO:0034332 adherens junction organization
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Actin Gamma antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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