Actin Beta antibody

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Human anti Actin Beta

Product Type
PrecisionAb Monoclonal
Isotype
HuCAL Fab bivalent
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00014
Datasheet
WB
SDS
100 µl loader

Human anti Human actin beta antibody recognizes the product of the ACTB gene, cytoplasmic actin beta, also known as actin cytoplasmic 1. Actin beta is ubiquitously expressed in all eukaryotic cells, playing a critical role in cell survival, motility and re-modelling.

Actin beta is highly conserved throughout evolution, with all vertebrate and invertebrate species reviewed to date bearing a 98% or greater amino acid primary sequence identity to human actin beta. Plant species also exhibit a high degree of primary sequence conservation displaying considerable similarity to animal actin beta. For example, nearly 90% sequence identity is observed between the human ACTB and the model plant species Arabidopsis thaliana (mouse eared cress) ACT1 (Arabidopsis) gene products.

Actin beta is a commonly used western blotting loading control for cell and tissue lysates across many species due to its ubiquitous expression and evolutionary conservation rendering antibodies against this target cross reactive with protein preparations from many different organisms.

Human anti Human actin beta antibody recognizes human actin beta as a single band of ~42 kDa in multiple human and rodent cell line lysates by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti actin beta antibody detects a band of approximately 42 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
Preparation
Recombinant human monoclonal antibody purified by Strep-Tactin affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified human beta actin

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
P60709
Entrez Gene
ACTB
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Regulatory
For research purposes only

Applications of Actin Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Strep-Tag Classic:HRP MCA2489P E WB 75 µl loader
Goat anti Human IgG F(ab')2:HRP STAR126P E WB 0.5 ml loader

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