Actin Alpha 2 antibody

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Anti Actin Alpha 2 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with actin alpha 2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc; MP with 31 second exposure. Arrow points to actin alpha 2 (molecular weight 42 kDa).

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Mouse anti Actin Alpha 2

Product Type: PrecisionAb Monoclonal
Isotype: IgG2b
Format: Purified
Specificity: Actin Alpha 2

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Mouse anti Human actin alpha 2 antibody recognizes actin alpha 2 also known as alpha-cardiac actin, aortic smooth muscle actin or cell growth-inhibiting gene 46 protein.

The protein encoded by ACTA2 gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in ACTA2 gene cause aortic aneurysm familial thoracic type 6 (AAT6). Multiple alternatively spliced variants, encoding the same protein, have been identified (provided by RefSeq, Nov 2008).

Mouse anti Human actin alpha 2 antibody detects a band of 42 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti actin alpha 2 detects a band of approximately 42 kDa in HeLa cell lysate.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: E. coli derived recombinant protein corresponding to amino acids 1-377 of human actin alpha 2
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.