Actin Alpha 2 antibody

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Mouse anti Actin Alpha 2

Product Type
PrecisionAb Monoclonal
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00513
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human actin alpha 2 antibody recognizes actin alpha 2 also known as alpha-cardiac actin, aortic smooth muscle actin or cell growth-inhibiting gene 46 protein.

The protein encoded by ACTA2 gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in ACTA2 gene cause aortic aneurysm familial thoracic type 6 (AAT6). Multiple alternatively spliced variants, encoding the same protein, have been identified (provided by RefSeq, Nov 2008).

Mouse anti Human actin alpha 2 antibody detects a band of 42 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti actin alpha 2 detects a band of approximately 42 kDa in HeLa cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli derived recombinant protein corresponding to amino acids 1-377 of human actin alpha 2

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P62736 Related reagents
Entrez Gene
ACTA2 Related reagents
GO Terms
GO:0009615 response to virus
GO:0005829 cytosol
GO:0005524 ATP binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Actin Alpha 2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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