Mouse anti Human Acetylcholine Receptor beta antibody, clone B3 exhibits homogenous, high avidity binding to the beta subunit of nicotinc AChR, an integral membrane and cholinergic receptor, consisting of four further subunits: alpha, delta, gamma (immature muscle) and epsilon (mature muscle).
The binding of the neurotransmitter acetylcholine to AChR, results in conformational change affecting all subunits, and initiates the opening of an ion-conducting channel across the plasma membrane of certain neurons, and neuromuscular junctions.
Mouse anti Human Acetylcholine Receptor beta antibody, clone B3 has been shown to bind to recombinant AChR beta in Western blotting
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Human Acetylcholine Receptor (AChR).
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch
- Entrez Gene
- GO Terms
postsynaptic membrane organization
nicotinic acetylcholine-gated receptor-channel complex
synaptic transmission, cholinergic
neuromuscular synaptic transmission
behavioral response to nicotine
regulation of membrane potential
muscle fiber development
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of AChR Beta antibody
|Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Negative Isotype Controls Available
Useful Reagents Available
Product Specific References
References for AChR Beta antibody
Whiting, P.J. et al. (1986) Monoclonal antibodies that distinguish between normal and denervated human acetylcholine receptor.
J Neuroimmunol. 11 (3): 223-35.
Roberts, A. et al. (1992) Search for cross reactive idiotypes on monoclonal and myasthenia gravis acetylcholine receptor antibodies.
Autoimmunity 21: 53-60.
Heidenreich F. et al. (1988) Differences in fine specificity of anti-acetylcholine receptor antibodies between subgroups of spontaneous myasthenia gravis of recent onset and of penicillamine induced myasthenia.
Autoimmunity 2: 31-37.
Whiting, P.J. et al. (1986) Myasthenia gravis: monoclonal antihuman acetylcholine receptor antibodies used to analyze antibody specificities and responses to treatment.
Neurology. 36 (5): 612-7.
Schluep, M. et al. (1987) Acetylcholine receptors in human thymic myoid cells in situ: an immunohistological study.
Ann Neurol. 22 (2): 212-22.