AChR Beta antibody | B3

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Mouse anti Human Acetylcholine Receptor Beta

Product Type
Monoclonal Antibody
Clone
B3
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1329GA C F IP WB 0.1 mg
Mouse anti Human Acetylcholine Receptor beta antibody, clone B3 exhibits homogenous, high avidity binding to the beta subunit of nicotinc AChR, an integral membrane and cholinergic receptor, consisting of four further subunits: alpha, delta, gamma (immature muscle) and epsilon (mature muscle).

The binding of the neurotransmitter acetylcholine to AChR, results in conformational change affecting all subunits, and initiates the opening of an ion-conducting channel across the plasma membrane of certain neurons, and neuromuscular junctions.

Mouse anti Human Acetylcholine Receptor beta antibody, clone B3 has been shown to bind to recombinant AChR beta in Western blotting .

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Chicken
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Human Acetylcholine Receptor (AChR).
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from the date of despatch

More Information

UniProt
P11230 Related reagents
Entrez Gene
CHRNB1 Related reagents
GO Terms
GO:0001941 postsynaptic membrane organization
GO:0006936 muscle contraction
GO:0004872 receptor activity
GO:0005892 nicotinic acetylcholine-gated receptor-channel complex
GO:0007271 synaptic transmission, cholinergic
GO:0007274 neuromuscular synaptic transmission
GO:0030054 cell junction
GO:0035095 behavioral response to nicotine
GO:0042166 acetylcholine binding
GO:0042391 regulation of membrane potential
GO:0045211 postsynaptic membrane
GO:0048747 muscle fiber development
Regulatory
For research purposes only

Applications of AChR Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunoprecipitation
Western Blotting 1/100 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Product Specific References

References for AChR Beta antibody

  1. Whiting, P.J. et al. (1986) Monoclonal antibodies that distinguish between normal and denervated human acetylcholine receptor.
    J Neuroimmunol. 11 (3): 223-35.
  2. Roberts, A. et al. (1992) Search for cross reactive idiotypes on monoclonal and myasthenia gravis acetylcholine receptor antibodies.
    Autoimmunity 21: 53-60.
  3. Heidenreich F. et al. (1988) Differences in fine specificity of anti-acetylcholine receptor antibodies between subgroups of spontaneous myasthenia gravis of recent onset and of penicillamine induced myasthenia.
    Autoimmunity 2: 31-37.
  4. Whiting, P.J. et al. (1986) Myasthenia gravis: monoclonal antihuman acetylcholine receptor antibodies used to analyze antibody specificities and responses to treatment.
    Neurology. 36 (5): 612-7.
  5. Schluep, M. et al. (1987) Acetylcholine receptors in human thymic myoid cells in situ: an immunohistological study.
    Ann Neurol. 22 (2): 212-22.