ACAT1 antibody | AB02/4H6

100% Secure

Mouse anti ACAT1

Product Type
PrecisionAb Monoclonal
Product Code Applications Pack Size List Price Quantity
100 µl loader

Mouse anti ACAT1 antibody recognizes acetyl-CoA acetyltransferase, mitochondrial, also known as ACAT, MAT. ACAT1 is one of two ACAT proteins, ACAT1 is the mitochondrial ACAT protein whereas ACAT2 is cytosolic. ACAT1 is ubiquitously expressed, but especially expressed in steroidogenic organs and macrophages. This protein appears to be involved with catalyzing specific reactions of ketogenesis and ketolysis, and is also involved with isoleucine degradation (Goudarzi et al. 2019). Because ketone bodies are an important source of energy during low nutrient intake, ACAT1 is involved with the cellular response to fasting or starvation (Chen et al. 2019). Loss of ACAT1 in bone marrow cells has been found to attenuate atherosclerosis (Yamazaki et al. 2019). In addition, blocking ACAT1 in a mouse model appears to have multiple benefits for Alzheimer's Disease, and has been suggested as a therapeutic target. Blocking ACAT1 has been found to upregulate autophagy and diminishes the production of amyloid-beta peptides, peptides which trigger neurotoxicity along with tau (Shibuya et al. 2015).

Product Details

Target Species
Western Blotting
Mouse anti ACAT1 detects a band of approximately 40 kDa in HepG2 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
E. coli-derived recombinant protein of amino acids 134-427 of human ACAT1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Store undiluted at -20°C, avoiding repeated freeze thaw cycles
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0005515 protein binding
GO:0003985 acetyl-CoA C-acetyltransferase activity
GO:0046872 metal ion binding
GO:0005759 mitochondrial matrix
GO:0009083 branched chain family amino acid catabolic process
GO:0043441 acetoacetic acid biosynthetic process
GO:0046951 ketone body biosynthetic process
GO:0046952 ketone body catabolic process
PrecisionAb is a trademark of Bio-Rad Laboratories
For research purposes only

Applications of ACAT1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra