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A2M antibody | M5

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Anti Human A2M Antibody, clone M5 thumbnail image 1
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Sandwich ELISA analysis of human A2M using Mouse anti Human A2M (MCA6216GA) as a capture reagent and biotinylated Mouse anti Human A2M (MCA6217B) as a detection reagent with recombinant human A2M as the antigen. Detection was performed using HRP conjugated streptavidin and substrate.

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Mouse anti Human A2M:Biotin

Product Type
Monoclonal Antibody
Clone
M5
Isotype
IgG1
Specificity
A2M

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Product Code Applications Pack Size List Price Your Price Qty
MCA6217B
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E 0.1 mg loader
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Mouse anti Human A2M antibody, clone M5, recognizes alpha-2-macroglobulin (A2M), also known as CPAMD5. A2M is a major proteinase inhibitor, with the ability to bind most proteinases and many growth factors, hormones and cytokines in human blood and tissue (Kurz et al. 2017). A2M is largely produced by the liver under conditions of stress, and is then secreted into the blood and extracellular spaces. It is also produced locally by macrophages, fibroblasts, and epithelial cells (Kim et al. 2017).

A2M levels dramatically decrease as humans age, a process which has been hypothesized to facilitate tumor development. In naked mole rats, a species with pronounced resistance to cancer, A2M has been shown to inhibit tumor growth regardless of tumor origin (Kurz et al. 2017).

The biotinylated Mouse anti Human A2M antibody, clone M5 (MCA6217B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human A2M antibody, clone M17 (MCA6216GA) as the capture antibody.

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. coli derived recombinant human A2M (Ser24-Ala1474)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
ELISA Matched Pair(s)
ELISA Matched Pair-Capture Antibody: MCA6216GA
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
P01023
Entrez Gene
A2M
GO Terms
GO:0001869 negative regulation of complement activation, lectin pathway
GO:0002576 platelet degranulation
GO:0005829 cytosol
GO:0004867 serine-type endopeptidase inhibitor activity
GO:0005096 GTPase activator activity
GO:0005615 extracellular space
GO:0007264 small GTPase mediated signal transduction
GO:0007597 blood coagulation, intrinsic pathway
GO:0019899 enzyme binding
GO:0019959 interleukin-8 binding
GO:0019966 interleukin-1 binding
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0043120 tumor necrosis factor binding
GO:0051056 regulation of small GTPase mediated signal transduction

MCA6217B

100005237

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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