14-3-3 Theta antibody | AB03/2D8

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Mouse anti 14-3-3 Theta

Product Type
PrecisionAb Monoclonal
Clone
AB03/2D8
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00537
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human 14-3-3 theta antibody recognizes the 14-3-3 protein theta, also known as 14-3-3 protein T-cell, 14-3-3 protein tau, 14-3-3 protein theta or tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide.

The YWHAQ gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. YWHAQ expression is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease (provided by RefSeq, Jul 2008).

Mouse anti Human 14-3-3 protein theta antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti 14-3-3 theta detects a band of approximately 28 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human 14-3-3 theta (amino acids 1 - 245)

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P27348 Related reagents
Entrez Gene
YWHAQ Related reagents
GO Terms
GO:0005634 nucleus
GO:0005813 centrosome
GO:0019904 protein domain specific binding
GO:0045892 negative regulation of transcription, DNA-dependent
GO:0047485 protein N-terminus binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of 14-3-3 Theta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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