14-3-3 Epsilon antibody | AB01/1G9

Mouse anti 14-3-3 Epsilon

Product Type
PrecisionAb Monoclonal
Clone
AB01/1G9
Isotype
IgG2a
Format
Purified
Specificity
14-3-3 Epsilon

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VMA00525
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SDS Safety Datasheet SDS
WB 100 µl loader
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Mouse anti Human 14-3-3 epsilon antibody recognizes the 14-3-3 protein epsilon, also known as epididymis luminal protein 2, mitochondrial import stimulation factor L subunit, protein kinase C inhibitor protein-1, or tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide, or YWHAE.

YWHAE gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for YWHAE (provided by RefSeq, Aug 2008).

Mouse anti Human 14-3-3 epsilon antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti 14-3-3 epsilon detects a band of approximately 28 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli-derived recombinant human 14-3-3 epsilon (amino acids 1 - 255)
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P62258
Entrez Gene
YWHAE
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