IgA antibody | K129.2G5
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E ResetMouse anti Horse IgA
- Product Type
- Monoclonal Antibody
- Clone
- K129.2G5
- Isotype
- IgG1
- Specificity
- IgA
Antibody Quality and Performance Guarantee
| Mouse anti Horse IgA antibody, clone K129.2G5 recognizes equine IgA and does not cross-react with equine IgG or IgM. |
- Target Species
- Horse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Equine IgA
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the mouse P3/X63/Ag8.653 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/20,000 | 1/200,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for IgA antibody
-
Flock, M. et al. (2004) Recombinant Streptococcus equi proteins protect mice in challenge experiments and induce immune response in horses.
Infect Immun. 72 (6): 3228-36. -
Guss, B. et al. (2009) Getting to grips with strangles: an effective multi-component recombinant vaccine for the protection of horses from Streptococcus equi infection.
PLoS Pathog. 5: e1000584 -
Tallmadge, R.L. et al. (2009) Expression of essential B cell genes and immunoglobulin isotypes suggests active development and gene recombination during equine gestation.
Dev Comp Immunol. 33 (9): 1027-38. -
Jonsdottir, S. et al. (2016) A preventive immunization approach against insect bite hypersensitivity: Intralymphatic injection with recombinant allergens in Alum or Alum and monophosphoryl lipid A.
Vet Immunol Immunopathol. 172: 14-20. -
Palm, A.E. et al. (2016) Secretory immunoglobulin A and immunoglobulin G in horse saliva.
Vet Immunol Immunopathol. 180: 59-65.
MCA629GA
1605
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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