Mouse anti Horse IgA antibody, clone K129.2G5 recognizes equine IgA and does not cross-react with equine IgG or IgM.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Equine IgA
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the mouse P3/X63/Ag8.653 myeloma cell line
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IgA antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Product Specific References
References for IgA antibody
Guss, B. et al. (2009) Getting to grips with strangles: an effective multi-component recombinant vaccine
for the protection of horses from Streptococcus equi infection.
PLoS Pathog. 5: e1000584
Jonsdottir, S. et al. (2016) A preventive immunization approach against insect bite hypersensitivity: Intralymphatic injection with recombinant allergens in Alum or Alum and monophosphoryl lipid A.
Veterinary Immunology and Immunopathology. 172: 14-20.
Tallmadge, R.L. et al. (2009) Expression of essential B cell genes and immunoglobulin isotypes suggests active development and gene recombination during equine gestation.
Dev Comp Immunol. 33 (9): 1027-38.
Flock, M. et al. (2004) Recombinant Streptococcus equi proteins protect mice in challenge experiments and induce immune response in horses.
Infect Immun. 72 (6): 3228-36.
Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
Vet Immunol Immunopathol. 62 (2): 101-43.
Palm, A.E. et al. (2016) Secretory immunoglobulin A and immunoglobulin G in horse saliva.
Vet Immunol Immunopathol. 180: 59-65.
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