T Lymphocytes antibody | CT5
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Mouse anti Guinea Pig T Lymphocytes antibody, clone CT5 recognizes a cell surface antigen present on the surface of all guinea pig T lymphocytes. Reactivity is also seen with some myeloid cells, and with the L2C guinea pig B cell leukaemia.
Product Details
- Target Species
- Guinea Pig
- Product Form
- Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Guinea pig peritoneal T cells.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of reconstitution.
- Shelf Life
- 18 months from the date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of T Lymphocytes antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/5 | 1/10 | |
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:APC | MCA928APC | 100 Tests | F | ||
| Mouse IgG1 Negative Control:FITC | MCA928F | 100 Tests | F |
Product Specific References
References for T Lymphocytes antibody
-
Tan, B.T. et al. (1985) Production of monoclonal antibodies defining guinea pig T-cell surface markers and a strain 13 Ia-like antigen: the value of immunohistological screening.
Hybridoma. 4 (2): 115-24. -
Steerenberg, P.A. et al. (1991) Tumour rejection after adoptive transfer of line-10-immune spleen cells is mediated by two T cell subpopulations.
Cancer Immunol Immunother. 34 (2): 103-10. -
Steerenberg, P.A. et al. (1990) Tumor infiltrating leukocytes (tils) during progressive tumor growth and BCG-mediated tumor regression.
Virchows Arch B Cell Pathol Incl Mol Pathol. 59 (4): 185-94. -
Shang, S. et al. (2011) Activities of TMC207, rifampin, and pyrazinamide against Mycobacterium tuberculosis infection in guinea pigs.
Antimicrob Agents Chemother. 55 (1): 124-31.
