T Lymphocytes antibody | CT5
Mouse anti Guinea Pig T Lymphocytes antibody, clone CT5 (MCA751) used for the identification of T-lymphocytes in guinea pig nasal septum tissue sections by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Impact of PPS and budesonide on histological nasal tissue parameters during the allergic late phase reaction. Epithelial nasal tissue T lymphocytes are shown in G. (F) Hematoxylin stained nasal mucosa to exemplify the overall increased leukocyte tissue infiltration in allergen‐challenged animals (infiltrating leukocytes are exemplified by arrows). Epithelial loss of PAS % mucin content is shown in H. OVA, ovalbumin; Sal, saline control. Bar codes in E–G = 70μm, H = 50μm.
From: Sanden C, Mori M, Jogdand P, Jönsson J, Krishnan R, Wang X, Erjefält JS.
Broad Th2 neutralization and anti-inflammatory action of pentosan polysulfate sodium in experimental allergic rhinitis.
Immun Inflamm Dis. 2017 Sep;5(3):300-9.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Guinea Pig T Lymphocytes antibody, clone CT5 recognizes a cell surface antigen present on the surface of all guinea pig T lymphocytes. It is likely that CT5 binds to the guinea pig homolog of human CD3 (Miszczyk et al. 2014).
Product Details
- Target Species
- Guinea Pig
- Product Form
- Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Guinea pig peritoneal T cells.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of T Lymphocytes antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/5 | 1/10 | |
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:APC | MCA928APC | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
Product Specific References
References for T Lymphocytes antibody
-
Tan, B.T. et al. (1985) Production of monoclonal antibodies defining guinea pig T-cell surface markers and a strain 13 Ia-like antigen: the value of immunohistological screening.
Hybridoma. 4 (2): 115-24. -
Steerenberg, P.A. et al. (1991) Tumour rejection after adoptive transfer of line-10-immune spleen cells is mediated by two T cell subpopulations.
Cancer Immunol Immunother. 34 (2): 103-10. -
Steerenberg, P.A. et al. (1990) Tumor infiltrating leukocytes (tils) during progressive tumor growth and BCG-mediated tumor regression.
Virchows Arch B Cell Pathol Incl Mol Pathol. 59 (4): 185-94. -
Shang, S. et al. (2011) Activities of TMC207, rifampin, and pyrazinamide against Mycobacterium tuberculosis infection in guinea pigs.
Antimicrob Agents Chemother. 55 (1): 124-31. -
Sanden, C. et al. (2017) Broad Th2 neutralization and anti-inflammatory action of pentosan polysulfate sodium in experimental allergic rhinitis.
Immun Inflamm Dis. 5 (3): 300-9. -
Wagner, A.H. et al. (2008) Signal transducer and activator of transcription 1 decoy oligodeoxynucleotide suppression of contact hypersensitivity.
J Allergy Clin Immunol. 121 (1): 158-65.e5. -
Wu, W.H. et al. (2012) Immune status and the development of Listeria monocytogenes infection in aged and young guinea pigs.
Clin Invest Med. 35 (5): E309.
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