T Lymphocytes antibody | CT5

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Mouse anti Guinea Pig T Lymphocytes:APC

Mouse anti Guinea Pig T Lymphocytes:FITC

Product Type
Monoclonal Antibody
Clone
CT5
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA751APC F 100 Tests
MCA751F F 100 Tests
Mouse anti Guinea Pig T Lymphocytes antibody, clone CT5 recognizes a cell surface antigen present on the surface of all guinea pig T lymphocytes. Reactivity is also seen with some myeloid cells, and with the L2C guinea pig B cell leukaemia.

Product Details

Target Species
Guinea Pig
Product Form
Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Guinea pig peritoneal T cells.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC. DO NOT FREEZE This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Shelf Life
18 months from the date of despatch.

More Information

Regulatory
For research purposes only

Applications of T Lymphocytes antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5 1/10
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:APC MCA928APC 100 Tests F
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F

Product Specific References

References for T Lymphocytes antibody

  1. Tan, B.T. et al. (1985) Production of monoclonal antibodies defining guinea pig T-cell surface markers and a strain 13 Ia-like antigen: the value of immunohistological screening.
    Hybridoma. 4 (2): 115-24.
  2. Steerenberg, P.A. et al. (1991) Tumour rejection after adoptive transfer of line-10-immune spleen cells is mediated by two T cell subpopulations.
    Cancer Immunol Immunother. 34 (2): 103-10.
  3. Steerenberg, P.A. et al. (1990) Tumor infiltrating leukocytes (tils) during progressive tumor growth and BCG-mediated tumor regression.
    Virchows Arch B Cell Pathol Incl Mol Pathol. 59 (4): 185-94.
  4. Shang, S. et al. (2011) Activities of TMC207, rifampin, and pyrazinamide against Mycobacterium tuberculosis infection in guinea pigs.
    Antimicrob Agents Chemother. 55 (1): 124-31.