CD90 antibody | CT4
Mouse anti Guinea Pig CD90 antibody, clone CT4 (MCA750) used for the detection of CD90 (homing receptor) expressing cells in the guinea pig lungs by flow cytometry
Image caption:
Monitoring of activated T cells in the infected lungs during therapy. Percentage of activated CD4+ T cells (A) and CD8+ T cells (B) in drug or drug plus MIP group were evaluated at different time points during the course of therapy. The percentage of respective cells in infected control group has been shown in the inset of bar diagram. Activated T cells were analyzed based on the expression of homing receptors as shown in the representative contour plots and compared at 15 days and 30 days of therapy. In each plot, region R6 represents T cells with high homing receptor expression and the corresponding percentage value is mentioned inside the region. Data represents the mean value of four animals in each group. * p<0.05. Uninfected animal (naive) was taken as uninfected negative control and untreated animal at 30 days post infection was taken as infected positive control.
From: Gupta A, Ahmad FJ, Ahmad F, Gupta UD, Natarajan M, et al. (2012)
Efficacy of Mycobacterium indicus pranii Immunotherapy as an Adjunct to Chemotherapy for Tuberculosis and Underlying Immune Responses in the Lung.
PLoS ONE 7(7): e39215.
Mouse anti Guinea Pig CD90 antibody, clone CT4 recognizes guinea pig THY-1 (CD90), a small but heavily glycosylated member of the immunoglobulin superfamily. Clone CT4 was originally identified as recognizing a guinea pig homing receptor (Kraal et al. 1986) and later confirmed as recognizing the guinea pig homologue of human and rodent CD90 (Schäfer et al. 1999) by purification and microsequencing. Guinea Pig CD90 is notable for its level of N-linked glycosylation, rendering it an apparent molecular mass of ~36 kDa, higher than that seen in most other species where CD90 has been identified and characterized.
Guinea pig CD90 (according to Uniprot entry Q9WUR5) demonstrates 82% amino acid identity with human CD90, 74% with murine CD90 and 76% with rat.
Guinea pig CD90 is highly expressed in the brain and unlike other species is also expressed on most resting B lymphocytes (Schäfer et al. 1999)
Mouse anti Guinea Pig CD90 antibody, clone CT4 has been used extensively for the flow cytometric analysis of guinea pig cell populations (Gupta et al. 2012).
Guinea pig CD90 (according to Uniprot entry Q9WUR5) demonstrates 82% amino acid identity with human CD90, 74% with murine CD90 and 76% with rat.
Guinea pig CD90 is highly expressed in the brain and unlike other species is also expressed on most resting B lymphocytes (Schäfer et al. 1999)
Mouse anti Guinea Pig CD90 antibody, clone CT4 has been used extensively for the flow cytometric analysis of guinea pig cell populations (Gupta et al. 2012).
Product Details
- Target Species
- Guinea Pig
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
0.7% Bovine Serum Albumin - Immunogen
- Peritoneal T-cells.
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of CD90 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/100 | 1/1000 | |
| Immunohistology - Frozen | 1/100 | 1/1000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | 0.5 mg | F | ||
| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | 1 ml | F | ||
| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | 1 mg | C E P RE WB | ||
| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | 1 mg | F |
Product Specific References
References for CD90 antibody
-
Tan, B.T. et al. (1985) Production of monoclonal antibodies defining guinea pig T-cell surface markers and a strain 13 Ia-like antigen: the value of immunohistological screening.
Hybridoma. 4 (2): 115-24. -
Kraal, G. et al. (1986) A surface molecule on guinea pig lymphocytes involved in adhesion and homing.
Eur J Immunol. 16 (12): 1515-9. -
Schäfer, H. et al. (1991) T cell proliferation induced by monoclonal antibodies to a phosphatidylinositol-linked differentiation antigen of guinea pig lymphocytes.
Eur J Immunol. 21 (3): 701-5. -
Gupta, A. et al. (2012) Efficacy of Mycobacterium indicus pranii immunotherapy as an adjunct to chemotherapy for tuberculosis and underlying immune responses in the lung.
PLoS One. 7: e39215. -
Gupta, A. et al. (2012) Protective efficacy of Mycobacterium indicus pranii against tuberculosis and underlying local lung immune responses in guinea pig model.
Vaccine. 30: 6198-209. -
Shang, S. et al. (2011) Activities of TMC207, rifampin, and pyrazinamide against Mycobacterium tuberculosis infection in guinea pigs.
Antimicrob Agents Chemother. 55 (1): 124-31.
