CD90 antibody | CT4

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Mouse anti Guinea Pig CD90

Product Type
Monoclonal Antibody
Clone
CT4
Isotype
IgG3
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA750 C F 0.25 ml
Mouse anti Guinea Pig CD90 antibody, clone CT4 recognizes guinea pig THY-1 (CD90), a small but heavily glycosylated member of the immunoglobulin superfamily. Clone CT4 was originally identified as recognizing a guinea pig homing receptor (Kraal et al. 1986) and later confirmed as recognizing the guinea pig homologue of human and rodent CD90 (Schäfer et al. 1999) by purification and microsequencing. Guinea Pig CD90 is notable for its level of N-linked glycosylation, rendering it an apparent molecular mass of ~36 kDa, higher than that seen in most other species where CD90 has been identified and characterized.

Guinea pig CD90 (according to Uniprot entry Q9WUR5) demonstrates 82% amino acid identity with human CD90, 74% with murine CD90 and 76% with rat.

Guinea pig CD90 is highly expressed in the brain and unlike other species is also expressed on most resting B lymphocytes (Schäfer et al. 1999)

Mouse anti Guinea Pig CD90 antibody, clone CT4 has been used extensively for the flow cytometric analysis of guinea pig cell populations (Gupta et al. 2012).

Product Details

Target Species
Guinea Pig
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.7% Bovine Serum Albumin
Immunogen
Peritoneal T-cells.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of CD90 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/1000
Immunohistology - Frozen 1/100 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for CD90 antibody

  1. Tan, B.T. et al. (1985) Production of monoclonal antibodies defining guinea pig T-cell surface markers and a strain 13 Ia-like antigen: the value of immunohistological screening.
    Hybridoma. 4 (2): 115-24.
  2. Kraal, G. et al. (1986) A surface molecule on guinea pig lymphocytes involved in adhesion and homing.
    Eur J Immunol. 16 (12): 1515-9.
  3. Schäfer, H. et al. (1991) T cell proliferation induced by monoclonal antibodies to a phosphatidylinositol-linked differentiation antigen of guinea pig lymphocytes.
    Eur J Immunol. 21 (3): 701-5.
  4. Gupta, A. et al. (2012) Efficacy of Mycobacterium indicus pranii immunotherapy as an adjunct to chemotherapy for tuberculosis and underlying immune responses in the lung.
    PLoS One. 7: e39215.
  5. Gupta, A. et al. (2012) Protective efficacy of Mycobacterium indicus pranii against tuberculosis and underlying local lung immune responses in guinea pig model.
    Vaccine. 30: 6198-209.
  6. Shang, S. et al. (2011) Activities of TMC207, rifampin, and pyrazinamide against Mycobacterium tuberculosis infection in guinea pigs.
    Antimicrob Agents Chemother. 55 (1): 124-31.