Bu-1a/b antibody | BoA1
Mouse anti Guinea Fowl Bu-1a/b antibody, clone BoA1 specifically recognizes the avian bursal cell antigen, Bu-1a/b. Bu-1a/b, also known as chB6, is a type 1 transmembrane protein.
Bu-1 is a product of two alleles, Bu-1a (chB6.1) and Bu-1b (chB6.2).Mouse anti Guinea Fowl Bu-1a/b antibody, clone BoA1 recognizes both of these alleles. The Bu-1 antigen is expressed by chicken B-cells throughout most of their development and by a subset of monocytes and macrophages (Igyártó et al. 2008) but is absent from erythrocytes, granulocytes and thrombocytes. As such the Mouse anti Guinea Fowl Bu-1a/b antibody, clone BoA1 provides a useful marker of avian B-cells.
Clone BoA1 is unique in that while other antibodies generated against the Bu-1a/b antigen are restricted in their species reactivity, clone BoA1 recognizes the Bu-1a/b antigen in all phasianid species tested to date. Clone BoA1 therefore provides a reagent that facilitates comparative studies in both domestic and wild phasianids.
In addition to Mouse anti Guinea Fowl Bu-1a/b antibody, clone BoA1, clone AV20 which is specific to chicken Bu1-a/b and clone L22 which recognizes the Bu-1a but not the Bu-1b allele in chicken and quail, are also available from Bio-Rad.
Bu-1 is a product of two alleles, Bu-1a (chB6.1) and Bu-1b (chB6.2).Mouse anti Guinea Fowl Bu-1a/b antibody, clone BoA1 recognizes both of these alleles. The Bu-1 antigen is expressed by chicken B-cells throughout most of their development and by a subset of monocytes and macrophages (Igyártó et al. 2008) but is absent from erythrocytes, granulocytes and thrombocytes. As such the Mouse anti Guinea Fowl Bu-1a/b antibody, clone BoA1 provides a useful marker of avian B-cells.
Clone BoA1 is unique in that while other antibodies generated against the Bu-1a/b antigen are restricted in their species reactivity, clone BoA1 recognizes the Bu-1a/b antigen in all phasianid species tested to date. Clone BoA1 therefore provides a reagent that facilitates comparative studies in both domestic and wild phasianids.
In addition to Mouse anti Guinea Fowl Bu-1a/b antibody, clone BoA1, clone AV20 which is specific to chicken Bu1-a/b and clone L22 which recognizes the Bu-1a but not the Bu-1b allele in chicken and quail, are also available from Bio-Rad.
Product Details
- Target Species
- Guinea Fowl
- Species Cross-Reactivity
-
Target Species Cross Reactivity Quail Turkey Chicken Grey partridge - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin - Immunogen
- Bursal cell suspension from guinea fowl (Numida melagris).
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
Applications of Bu-1a/b antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Immunoprecipitation | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Chicken Bu-1A:FITC | MCA2170F | F | 0.1 mg |
|
|
| Mouse anti Chicken Bu-1a/b | MCA5764 | C F IP | 0.25 mg | ||
| Mouse anti Chicken CSF1R | MCA5956GA | C F IF | 0.1 mg |
|
Product Specific References
References for Bu-1a/b antibody
-
Igyártó, B.Z. et al. (2008) Identification of the avian B-cell-specific Bu-1 alloantigen by a novel monoclonal antibody.
Poult Sci. 87 (2): 351-5. -
Nagy, N. et al. (2009) Endothelial cells promote migration and proliferation of enteric neural crest cells via beta1 integrin signaling.
Dev Biol. 330 (2): 263-72. -
Nagy, N. & Oláh, I. (2010) Experimental evidence for the ectodermal origin of the epithelial anlage of the chicken bursa of Fabricius.
Development. 137 (18): 3019-23. -
Nagy, N. et al. (2016) Avian dendritic cells: Phenotype and ontogeny in lymphoid organs.
Dev Comp Immunol. 58: 47-59.
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