Bu-1a/b antibody | BoA1

Mouse anti Guinea Fowl Bu-1a/b

Product Type
Monoclonal Antibody
Clone
BoA1
Isotype
IgG1
Specificity
Bu-1a/b

Product Code Applications Pack Size List Price Your Price Qty
MCA5958GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C IP WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Guinea Fowl Bu-1a/b antibody, clone BoA1 recognizes the avian bursal cell antigen, Bu-1a/b. Bu-1a/b, also known as chB6, is a type 1 transmembrane protein.

Bu-1 is a product of two alleles, Bu-1a (chB6.1) and Bu-1b (chB6.2).Mouse anti Guinea Fowl Bu-1a/b antibody, clone BoA1 recognizes both of these alleles. The Bu-1 antigen is expressed by chicken B-cells throughout most of their development and by a subset of monocytes and macrophages (Igyártó et al. 2008) but is absent from erythrocytes, granulocytes and thrombocytes. As such the Mouse anti Guinea Fowl Bu-1a/b antibody, clone BoA1 provides a useful marker of avian B-cells.

Clone BoA1 is unique in that while other antibodies generated against the Bu-1a/b antigen are restricted in their species reactivity, clone BoA1 recognizes a conserved epitope of the Bu-1a/b antigen in both domestic and wild phasianid species

In addition to Mouse anti Guinea Fowl Bu-1a/b antibody, clone BoA1, clone AV20 (MCA5764) which is specific to chicken Bu1-a/b and clone L22 (MCA2170F) which recognizes the Bu-1a but not the Bu-1b allele in chicken and quail, are also available.

Target Species
Guinea Fowl
Species Cross-Reactivity
Target SpeciesCross Reactivity
Quail
Turkey
Chicken
Grey partridge
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin
Immunogen
Bursal cell suspension from guinea fowl (Numida melagris).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Goat anti Mouse IgG/A/M:Alk. Phos.
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

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Description Mouse anti Chicken Bu-1A:FITC
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References for Bu-1a/b antibody

  1. Igyártó, B.Z. et al. (2008) Identification of the avian B-cell-specific Bu-1 alloantigen by a novel monoclonal antibody.
    Poult Sci. 87 (2): 351-5.
  2. Nagy, N. et al. (2009) Endothelial cells promote migration and proliferation of enteric neural crest cells via beta1 integrin signaling.
    Dev Biol. 330 (2): 263-72.
  3. Nagy, N. & Oláh, I. (2010) Experimental evidence for the ectodermal origin of the epithelial anlage of the chicken bursa of Fabricius.
    Development. 137 (18): 3019-23.
  4. Nagy, N. et al. (2016) Avian dendritic cells: Phenotype and ontogeny in lymphoid organs.
    Dev Comp Immunol. 58: 47-59.

Synonyms
Bursal Antigen 1 A/B
UniProt
Q90747
Q90746
View more products with Bu-1a/b specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Guinea Fowl Products