Bu-1a/b antibody | BoA1

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Mouse anti Guinea Fowl Bu-1a/b

Product Type
Monoclonal Antibody
Clone
BoA1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5958GA C IP WB 0.1 mg
Mouse anti Guinea Fowl Bu-1a/b antibody, clone BoA1 specifically recognizes the avian bursal cell antigen, Bu-1a/b. Bu-1a/b, also known as chB6, is a type 1 transmembrane protein.

Bu-1 is a product of two alleles, Bu-1a (chB6.1) and Bu-1b (chB6.2).Mouse anti Guinea Fowl Bu-1a/b antibody, clone BoA1 recognizes both of these alleles. The Bu-1 antigen is expressed by chicken B-cells throughout most of their development and by a subset of monocytes and macrophages (Igyártó et al. 2008) but is absent from erythrocytes, granulocytes and thrombocytes. As such the Mouse anti Guinea Fowl Bu-1a/b antibody, clone BoA1 provides a useful marker of avian B-cells.

Clone BoA1 is unique in that while other antibodies generated against the Bu-1a/b antigen are restricted in their species reactivity, clone BoA1 recognises the Bu-1a/b antigen in all phasianid species tested to date. Clone BoA1 therefore provides a reagent that facilitates comparative studies in both domestic and wild phasianids.

In addition to Mouse anti Guinea Fowl Bu-1a/b antibody, clone BoA1, clone AV20 which is specific to chicken Bu1-a/b and clone L22 which recognizes the Bu-1a but not the Bu-1b allele in chicken and quail, are also available from Bio-Rad.

Product Details

Target Species
Guinea Fowl
Species Cross-Reactivity
Target SpeciesCross Reactivity
Quail
Turkey
Chicken
Grey partridge
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin
Immunogen
Bursal cell suspension from guinea fowl (Numida melagris).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch

More Information

UniProt
Q90747 Related reagents
Q90746 Related reagents
Regulatory
For research purposes only

Applications of Bu-1a/b antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Chicken Bu-1A:FITC MCA2170F 0.1 mg F
Mouse anti Chicken Bu-1A:RPE MCA2170PE 100 Tests F
Mouse anti Chicken Bu-1a/b MCA5764 0.25 mg C F IP
Mouse anti Chicken CSF1R MCA5956GA 0.1 mg C F IF

Product Specific References

References for Bu-1a/b antibody

  1. Igyártó, B.Z. et al. (2008) Identification of the avian B-cell-specific Bu-1 alloantigen by a novel monoclonal antibody.
    Poult Sci. 87 (2): 351-5.
  2. Nagy, N. et al. (2009) Endothelial cells promote migration and proliferation of enteric neural crest cells via beta1 integrin signaling.
    Dev Biol. 330 (2): 263-72.
  3. Nagy, N. & Oláh, I. (2010) Experimental evidence for the ectodermal origin of the epithelial anlage of the chicken bursa of Fabricius.
    Development. 137 (18): 3019-23.