Durvalumab antibody | AbD41327




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E ResetHuman anti Durvalumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD41327
- Isotype
- Fab antibody
- Specificity
- Durvalumab
Human Anti-Durvalumab Antibody, clone AbD41327ad is a paratope specific, inhibitory anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug durvalumab. The antibody does not recognize free PD-L1 nor durvalumab in complex with PD-L1. Anti-Durvalumab monovalent Fab antibody TZA004 has a SpyTag incorporated for conversion by the user into alternative antibody formats using any of the SpyCatchers available in our catalog. View a summary of all SpyCatcher products Anti-Durvalumab monovalent Fab antibody TZA004 may be used as the capture antibody for durvalumab in a PK bridging ELISA together with HCA360, clone AbD41313ia as the detection antibody to measure free durvalumab captured via immobilized PD-L1. PK bridging ELISA Durvalumab (Imfinzi) is a human IgG1/kappa antibody with allotype G1m3. The CH2 domain was engineered with three amino acid substitutions (L238F, L239E, P335S) to reduce the Fc-mediated effector functions to prevent depletion of PD-L1+ T-cells. It was first approved for previously treated patients with advanced bladder cancer. It was later approved for the treatment of unresectable stage 3 NSCLC (Non-Small Cell Lung Cancer) and ES-SCLC. Durvalumab binds to PD-L1 to block the interaction with the inhibitory T cell receptor PD-1. This blockade removes the suppression of T-cells that are specific for MHC-1-presented neoepitopes on tumor cells. Removal of this inhibitory signal leads to the release of granzymes and perforins by the activated T-cells, and finally to the destruction of the tumor cells. View a summary of all anti-durvalumab antibodies |
- Target Species
- Drug
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Durvalumab
- Affinity
- The monovalent intrinsic affinity of AbD41327ad was measured as KD = 3.6 nM by real time, label free molecular interaction analysis on immobilized durvalumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- ELISA Matched Pair(s)
-
ELISA Matched Pair-Detection Antibody:
HCA360
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Imfinzi is a registered trademark of Medimmune/AstraZeneca using Abgenix's XenoMouse technology.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
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ELISA | ![]() |
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD41327ad can be used as the capture antibody for durvalumab in a PK ELISA together with HCA360 as the detection antibody.
PK bridging ELISA
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | Log in | |||
List Price | Your Price | ||||||
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Description | LYNX Rapid HRP Antibody Conjugation Kit | ||||||
SpyTag3 Peptide | BLP086 | IY | 1 mg |
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List Price | Your Price | ||||||
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Description | SpyTag3 Peptide | ||||||
Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
List Price | Your Price | ||||||
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Description | Hispec Assay Diluent | ||||||
Human anti Durvalumab | HCA359 | E | 0.1 mg |
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List Price | Your Price | ||||||
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Description | Human anti Durvalumab |
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
TZA004

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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