Durvalumab antibody | AbD41327

Human anti Durvalumab

Product Type
Monoclonal Antibody
Clone
AbD41327
Isotype
Fab antibody
Specificity
Durvalumab

Product Code Applications Pack Size List Price Your Price Qty
New TZA004
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg
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Search for Batch Specific Datasheets

Human Anti-Durvalumab Antibody, clone AbD41327ad is a paratope specific, inhibitory anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug durvalumab. The antibody does not recognize free PD-L1 nor durvalumab in complex with PD-L1.

Anti-Durvalumab monovalent Fab antibody TZA004 has a SpyTag incorporated for conversion by the user into alternative antibody formats using any of the SpyCatchers available in our catalog.

View a summary of all SpyCatcher products

Anti-Durvalumab monovalent Fab antibody TZA004 may be used as the capture antibody for durvalumab in a PK bridging ELISA together with HCA360, clone AbD41313ia as the detection antibody to measure free durvalumab captured via immobilized PD-L1.

PK bridging ELISA

Durvalumab (Imfinzi) is a human IgG1/kappa antibody with allotype G1m3. The CH2 domain was engineered with three amino acid substitutions (L238F, L239E, P335S) to reduce the Fc-mediated effector functions to prevent depletion of PD-L1+ T-cells. It was first approved for previously treated patients with advanced bladder cancer. It was later approved for the treatment of unresectable stage 3 NSCLC (Non-Small Cell Lung Cancer) and ES-SCLC. Durvalumab binds to PD-L1 to block the interaction with the inhibitory T cell receptor PD-1. This blockade removes the suppression of T-cells that are specific for MHC-1-presented neoepitopes on tumor cells. Removal of this inhibitory signal leads to the release of granzymes and perforins by the activated T-cells, and finally to the destruction of the tumor cells.

View a summary of all anti-durvalumab antibodies

Target Species
Drug
Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
Immunogen
Durvalumab
Affinity
The monovalent intrinsic affinity of AbD41327ad was measured as KD = 3.6 nM by real time, label free molecular interaction analysis on immobilized durvalumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
ELISA Matched Pair(s)
ELISA Matched Pair-Detection Antibody: HCA360
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Imfinzi is a registered trademark of Medimmune/AstraZeneca using Abgenix's XenoMouse technology.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Clone AbD41327ad can be used as the capture antibody for durvalumab in a PK ELISA together with HCA360 as the detection antibody.

PK bridging ELISA

Description Product Code Applications Pack Size List Price Your Price Quantity
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
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Description LYNX Rapid HRP Antibody Conjugation Kit
SpyTag3 Peptide BLP086 IY 1 mg loader
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loader
Description SpyTag3 Peptide
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
Human anti Durvalumab HCA359 E 0.1 mg loader
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loader
Description Human anti Durvalumab

Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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