Durvalumab antibody | AbD41240

Human anti Durvalumab (Drug/Target Complex)

Product Type
Monoclonal Antibody
Clone
AbD41240
Isotype
Fab antibody
Specificity
Durvalumab
Region
DRUG/TARGET COMPLEX

Product Code Applications Pack Size List Price Your Price Qty
New TZA002
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg
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Search for Batch Specific Datasheets

Human Anti-Durvalumab (Drug/Target Complex) Antibody, clone AbD41240 specifically recognizes the antibody drug durvalumab when in complex with its target, PD-L1 (programmed cell death ligand 1). The antibody does not recognize free durvalumab nor unbound recombinant human PD-L1. It can be used to measure levels of durvalumab in patient samples.

Anti-Durvalumab monovalent Fab antibody TZA002 has a SpyTag incorporated for conversion by the user into alternative antibody formats using any of the SpyCatchers available in our catalog.

View a summary of all SpyCatcher products

Anti-Durvalumab monovalent Fab antibody TZA002 can be used to develop a pharmacokinetic (PK) antigen capture assay to measure free durvalumab captured via immobilized PD-L1.

PK bridging ELISA

Durvalumab (Imfinzi) is a human IgG1/kappa antibody with allotype G1m3. The CH2 domain was engineered with three amino acid substitutions (L238F, L239E, P335S) to reduce the Fc-mediated effector functions to prevent depletion of PD-L1+ T-cells. It was first approved for previously treated patients with advanced bladder cancer. It was later approved for the treatment of unresectable stage 3 NSCLC (Non-Small Cell Lung Cancer) and ES-SCLC. Durvalumab binds to PD-L1 to block the interaction with the inhibitory T cell receptor PD-1. This blockade removes the suppression of T-cells that are specific for MHC-1-presented neoepitopes on tumor cells. Removal of this inhibitory signal leads to the release of granzymes and perforins by the activated T-cells, and finally to the destruction of the tumor cells.

View a summary of all anti-durvalumab antibodies

Target Species
Drug
Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
Immunogen
Durvalumab
Affinity
The monovalent intrinsic affinity of AbD41240ad was measured as KD = 13 nM by real time, label free molecular interaction analysis on immobilized PD-L1/dupilumab complex.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Imfinzi is a trademark of Medimmune/AstraZeneca using Abgenix`s XenoMouse® technology.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Clone AbD41240ad can be used as the detection antibody in a PK bridging assay to measure durvalumab in complex with PD-L1.

Protocol: PK bridging ELISA

Description Product Code Applications Pack Size List Price Your Price Quantity
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
List Price Your Price
Description LYNX Rapid HRP Antibody Conjugation Kit
SpyTag3 Peptide BLP086 IY 1 mg loader
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loader
Description SpyTag3 Peptide
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent

Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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