CD34 antibody | 1H6
Mouse anti Dog CD34:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- 1H6
- Isotype
- IgG1
- Specificity
- CD34
Mouse anti dog CD34 antibody, clone 1H6 recognizes the canine homologue of CD34, a glycosylated type 1 transmembrane protein of approximately 110 kDa (McSweeney et al. 1998) expressed on the cell suface of endothelial cells and haematopoietic stem cells. Mouse anti dog CD34 antibody, clone 1H6 is a key marker of canine hematopoietic progenitor cells and is reported for use in CD34+ enrichment assays, (Goerner et al. 2001) and (Horn et al. 2004). |
- Target Species
- Dog
- Product Form
- Purified IgG conjugated to Alexa Fluor®647- liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Canine CD34 fusion protein.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS-1/FOX-NY myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse IgG1 Negative Control:Alexa Fluor® 647 |
Source Reference
-
McSweeney, P.A. et al. (1998) Characterization of monoclonal antibodies that recognize canine CD34.
Blood. 91 (6): 1977-86.
References for CD34 antibody
-
Goerner, M. et al. (1999) The use of granulocyte colony-stimulating factor during retroviral transduction on fibronectin fragment CH-296 enhances gene transfer into hematopoietic repopulating cells in dogs.
Blood. 94 (7): 2287-92. -
Bhattacharya, V. et al. (2000) Enhanced endothelialization and microvessel formation in polyester grafts seeded with CD34(+) bone marrow cells.
Blood. 95 (2): 581-5. -
Goerner, M. et al. (2001) Sustained multilineage gene persistence and expression in dogs transplanted with CD34(+) marrow cells transduced by RD114-pseudotype oncoretrovirus vectors.
Blood. 98 (7): 2065-70. -
Georges, G. et al. (2001) Engraftment of DLA-haploidentical marrow with ex vivo expanded, retrovirally transduced cytotoxic T lymphocytes.
Blood. 98:3447-55. -
Horn, P.A. et al. (2004) Efficient lentiviral gene transfer to canine repopulating cells using an overnight transduction protocol.
Blood. 103 (10): 3710-6. -
Avallone, G. et al. (2007) The spectrum of canine cutaneous perivascular wall tumors: morphologic, phenotypic and clinical characterization.
Vet Pathol. 44 (5): 607-20. -
Palmieri, C. et al. (2013) Use of electron microscopy to classify canine perivascular wall tumors.
Vet Pathol. 50 (2): 226-33. -
Bearden, R.N. et al. (2017) In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study.
Stem Cell Res Ther. 8 (1): 218.
View The Latest Product References
-
Trindade, A.B. et al. (2017) Mesenchymal-like stem cells in canine ovary show high differentiation potential.
Cell Prolif. Oct 08 [Epub ahead of print]. -
Lee, S.H. et al. (2016) Impact of local injection of brain-derived neurotrophic factor-expressing mesenchymal stromal cells (MSCs) combined with intravenous MSC delivery in a canine model of chronic spinal cord injury.
Cytotherapy. Oct 28 [Epub ahead of print]. -
Muir, P. et al. (2016) Autologous Bone Marrow-Derived Mesenchymal Stem Cells Modulate Molecular Markers of Inflammation in Dogs with Cruciate Ligament Rupture.
PLoS One. 11 (8): e0159095. -
Rajawat, Y.S. et al. (2021) In Vivo Gene Therapy for Canine SCID-X1 Using Cocal-Pseudotyped Lentiviral Vector.
Hum Gene Ther. 32 (1-2): 113-27. -
Grudzien, M. et al. (2021) A newly established canine NK-type cell line and its cytotoxic properties.
Vet Comp Oncol. 19 (3): 567-77. -
Tongu, E.A.O. et al. (2021) Allogenic mesenchymal stem cell-conditioned medium does not affect sperm parameters and mitigates early endometrial inflammatory responses in mares.
Theriogenology. 169: 1-8. -
Jaensch, S. et al. (2022) Clinicopathologic and immunophenotypic features in dogs with presumptive large granular lymphocyte leukaemia
Australian Veterinary Journal. [Epub ahead of print]. -
Salari Sedigh, H. et al. (2023) In vitro investigation of canine periodontal ligament-derived mesenchymal stem cells: A possibility of promising tool for periodontal regeneration.
J Oral Biol Craniofac Res. 13 (3): 403-11. -
Papa, P.M. et al. (2023) Intratesticular transplantation of allogenic mesenchymal stem cells mitigates testicular destruction after induced heat stress in Miniature-horse stallions.
J Equine Vet Sci. 132: 104961. -
Rezaei, M. et al. (2019) Transplantation of Bone Marrow-Derived Mesenchymal Stem Cells, Platelet-Rich Plasma, and Fibrin Glue for Periodontal Regeneration.
Int J Periodontics Restorative Dent. 39 (1): e32-e45. -
Yang, V.K. et al. (2021) Intravenous administration of allogeneic Wharton jelly-derived mesenchymal stem cells for treatment of dogs with congestive heart failure secondary to myxomatous mitral valve disease.
Am J Vet Res. 82 (6): 487-93. -
Crain, S.K. et al. (2019) Extracellular Vesicles from Wharton's Jelly Mesenchymal Stem Cells Suppress CD4 Expressing T Cells Through Transforming Growth Factor Beta and Adenosine Signaling in a Canine Model.
Stem Cells Dev. 28 (3): 212-26. -
Sheng, R. et al. (2023) Prognostic significance of CD25 expression in dogs with a noninvasive diagnosis of B-cell lymphoma treated with CHOP chemotherapy.
Vet Comp Oncol. 21 (1): 28-35. -
Millanta, F. et al. (2020) Cytologic grading of canine and feline spindle-cell sarcomas of soft tissues and its correlation with histologic grading.
Top Companion Anim Med. 41: 100458.
Further Reading
-
McSweeney, P. et al. (1996) Canine CD34: cloning of the cDNA and evaluation of an antiserum to recombinant protein.
Blood. 88:1992-2003.
- RRID
- AB_2074486
- UniProt
- Q28270
- Entrez Gene
- CD34
- GO Terms
- GO:0016021 integral to membrane
- GO:0030246 carbohydrate binding
- GO:0016337 cell-cell adhesion
- GO:0050900 leukocyte migration
MCA2411A647
150140 167451If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Dog ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up