CD107b antibody | AC17

Mouse anti Dog CD107b

Product Type
Monoclonal Antibody
Clone
AC17
Isotype
IgG1
Specificity
CD107b

Product Code Applications Pack Size List Price Your Price Qty
MCA2558GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F * IF IP WB 0.1 mg loader
List Price Your Price
loader
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Mouse anti Dog CD107b antibody, clone AC17 recognizes canine CD107b, otherwise known as lysosome-associated membrane protein 2 or LAMP-2. Immunofluorescence staining of MDCK cells with mouse anti dog CD107b, clone AC17 demonstrates staining patterns consistent with localization to lysozomes. This is supported by coincident staining of an exogenous lysozomal glycoprotein, avian LEP100 transfected into MDCK cells and detected using the anti LEP100 antibody clone CV24 (Nabi et al.1991).

Mouse anti Dog CD107b antibody, clone AC17 immunoprecipitates a protein of ~95 kDa in MDCK cells which, following Endo F digestion to remove N-linked oligosaccharides, yields a core protein product of 40 kDa, indicating the heavily glycosylated nature of CD107b. The molecular weight of canine CD107b is typical of many lysozome-associated membrane proteins. While most (97%) CD107b resides in the lysozomal environment in adherent MDCK cells in vitro, a small percentage is associated with the cell membrane (Nabi et al.1991).

CD107b has been shown to share high N-terminal amino acid sequence homology with human, mouse and rat CD107b (Nabi et al.1993).

Transfection of a mink type II lung epithelial cell line with beta1-6-N-acetylglucosaminyl transferase V demonstrates the formation of large lysozomal vacuoles, termed multilamellar bodies (MLBs), having a very distinct phenotype with expression of CD107b, as indicated by immunofluorescent staining with clone AC17. These MLBs require lysozomal degradation via an autophagic pathway for their formation and may have implications for lysozomal storage diseases (Hariri et al.2000). CD107b is involved in the lysosomal uptake of cytosolic proteins and the endocytic pathway.

Mouse anti Dog CD107b antibody, clone AC17 is suitable for use in electron microscopy (Nabi et al.1991).

Target Species
Dog
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Mink
Human
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
MDCK (Madin-Darby Canine Kidney) cells
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/50 1/200
Immunofluorescence
Immunoprecipitation
Western Blotting 1/100 1/500
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

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References for CD107b antibody

  1. Nabi, I.R. et al. (1991) An endogenous MDCK lysosomal membrane glycoprotein is targeted basolaterally before delivery to lysosomes.
    J Cell Biol. 115 (6): 1573-84.
  2. Nabi, I.R. & Rodriguez-Boulan, E. (1993) Increased LAMP-2 polylactosamine glycosylation is associated with its slower Golgi transit during establishment of a polarized MDCK epithelial monolayer.
    Mol Biol Cell. 4 (6): 627-35.
  3. Jou, T.S. et al. (2000) Selective alterations in biosynthetic and endocytic protein traffic in Madin-Darby canine kidney epithelial cells expressing mutants of the small GTPase Rac1.
    Mol Biol Cell. 11 (1): 287-304.
  4. Ihrke, G. et al. (2001) Competing sorting signals guide endolyn along a novel route to lysosomes in MDCK cells.
    EMBO J. 20 (22): 6256-64.
  5. Pluhar, G.E. et al. (2010) Anti-tumor immune response correlates with neurological symptoms in a dog with spontaneous astrocytoma treated by gene and vaccine therapy.
    Vaccine 28 (19): 3371-8.
  6. Cliffe, S.T. et al. (2009) SLC29A3 gene is mutated in pigmented hypertrichosis with insulin-dependent diabetes mellitus syndrome and interacts with the insulin signaling pathway.
    Hum Mol Genet. 18: 2257-65.
  7. Bai, Y. et al. (2011) Intracellular neutralization of viral infection in polarized epithelial cells by neonatal Fc receptor (FcRn)-mediated IgG transport.
    Proc Natl Acad Sci U S A. 108 (45): 18406-11.
  8. Nagahama, M. et al. (2011) Cellular vacuolation induced by Clostridium perfringens epsilon-toxin.
    FEBS J. 278: 3395-407.
  9. View The Latest Product References
  10. Nagahama, M. et al. (2012) Intracellular trafficking of Clostridium perfringens iota-toxin b.
    Infect Immun. 80: 3410-6.
  11. Hariri, M. et al. (2000) Biogenesis of multilamellar bodies via autophagy.
    Mol Biol Cell. 11: 255-68.

Further Reading

  1. Fukuda, M. (1991) Lysosomal membrane glycoproteins. Structure, biosynthesis, and intracellular trafficking.
    J Biol Chem. 266 (32): 21327-30.

Flow Cytometry

Immunofluorescence

Synonyms
LAMP-2
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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