Ubiquitin antibody | Ubi-1 (242.9)
Mouse anti bovine ubiquitin antibody, clone Ubi-1 is specific for ubiquitin an 8.5 kDa, 76 amino acid protein, highly conserved in eukaryotes. Clone Ubi-1 shows staining of tangle material in Alzheimer affected brain, and also stains other pathological inclusions such as Lewy bodies (in Parkinson's disease) and Pick bodies (in Pick's disease).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Human Mouse Rat Chicken Expected from Sequence Drosophila Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.06% Sodium Azide (NaN3)
- Purified ubiquitin (Bovine RBC) conjugated to Keyhole Limpet Haemocyanin.
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
Applications of Ubiquitin antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||1/250||1/1000|
|Immunohistology - Paraffin||1/250||1/1000|
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Alzheimers' diseased brain
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Human anti Mouse IgG1:HRP||HCA036P||0.1 mg||E|
|Rat anti Mouse IgG1||MCA1289||1 mg||E|
|Rat anti Mouse IgG Heavy Chain||MCA424||0.25 mg||E|
Product Specific References
References for Ubiquitin antibody
Meyer, E.M. et al. (1986) Antibodies directed against ubiquitin inhibit high affinity [3H]choline uptake in rat cerebral cortical synaptosomes.
J Biol Chem. 261 (31): 14365-8.
Perry, G. et al. (1987) Ubiquitin is detected in neurofibrillary tangles and senile plaque neurites of Alzheimer disease brains.
Proc Natl Acad Sci U S A. 84 (9): 3033-6.
Shaw, G. & Chau, V. (1988) Ubiquitin and microtubule-associated protein tau immunoreactivity each define distinct structures with differing distributions and solubility properties in Alzheimer brain.
Proc Natl Acad Sci U S A. 85 (8): 2854-8.
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