Ubiquitin antibody | Ubi-1 (242.9)

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Mouse anti Bovine Ubiquitin

Product Type
Monoclonal Antibody
Clone
Ubi-1 (242.9)
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
9400-1004 C P WB 0.1 ml
Mouse anti bovine ubiquitin antibody, clone Ubi-1 is specific for ubiquitin an 8.5 kDa, 76 amino acid protein, highly conserved in eukaryotes. Clone Ubi-1 shows staining of tangle material in Alzheimer affected brain, and also stains other pathological inclusions such as Lewy bodies (in Parkinson's disease) and Pick bodies (in Pick's disease).

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Mouse
Rat
Chicken Expected from Sequence
Drosophila Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.06% Sodium Azide (NaN3)
Immunogen
Purified ubiquitin (Bovine RBC) conjugated to Keyhole Limpet Haemocyanin.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P62990 Related reagents
Regulatory
For research purposes only

Applications of Ubiquitin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/250 1/1000
Immunohistology - Paraffin 1/250 1/1000
Western Blotting 1/250 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Alzheimers' diseased brain

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rat anti Mouse IgG1 MCA1289 1 mg E
Rat anti Mouse IgG Heavy Chain MCA424 0.25 mg E

Product Specific References

References for Ubiquitin antibody

  1. Meyer, E.M. et al. (1986) Antibodies directed against ubiquitin inhibit high affinity [3H]choline uptake in rat cerebral cortical synaptosomes.
    J Biol Chem. 261 (31): 14365-8.
  2. Perry, G. et al. (1987) Ubiquitin is detected in neurofibrillary tangles and senile plaque neurites of Alzheimer disease brains.
    Proc Natl Acad Sci U S A. 84 (9): 3033-6.
  3. Shaw, G. & Chau, V. (1988) Ubiquitin and microtubule-associated protein tau immunoreactivity each define distinct structures with differing distributions and solubility properties in Alzheimer brain.
    Proc Natl Acad Sci U S A. 85 (8): 2854-8.
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