Rhodopsin antibody | 1D4
Mouse anti Rhodopsin
- Product Type
- Monoclonal Antibody
- Clone
- 1D4
- Isotype
- IgG1
- Specificity
- Rhodopsin
Antibody Quality and Performance Guarantee
| Mouse anti Rhodopsin antibody, clone 1D4 recognizes the sequence TETSQVAPA corresponding to aa 340 to 348 of bovine rhodopsin, a pigment of the retina responsible for the formation of photoreceptor cells and the first events in the perception of light. Rhodopsin consists of the protein opsin and a reversibly bound cofactor, retinal, a photoreactive chromophore. Upon light absorption the 11-cis-retinal is isomerised to all-trans retinal, leading to a change in the shape of rhodopsin. The change activates the associated G protein and triggers a second messenger cascade, eventually resulting in the transmission of a nerve impulse to the brain. The trans-retinal is released and converted back into 11-cis-retinal for re-entry into the cycle. Defects in the gene cause various retinopathies such as retinitis pigmentosa and X-linked congenital stationary night blindness. |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mammals Expected from Sequence Amphibia Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- 10mM HEPES pH7.5, 150mM NaCl
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
0.01% Bovine Serum Albumin
50% Glycerol - Immunogen
- Bovine rhodopsin.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
||
| Immunohistology - Paraffin | |
1/100 | |
| Immunoprecipitation | |
||
| Western Blotting | |
1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Mouse retina
References for Rhodopsin antibody
-
Molday, R. S. et al. (1983) Monoclonal antibodies to rhodopsin: characterization, cross-reactivity, and application as structural probes.
Biochemistry 22: 653-60 -
MacKenzie, D. et al. (1984) Localization of binding sites for carboxyl terminal specific anti-rhodopsin monoclonal antibodies using synthetic peptides.
Biochemistry 23: 6544-9 -
Molday, R. S. et al. (1987) Peripherin. A rim-specific membrane protein of rod outer segment discs.
Invest Ophthalmol Vis Sci. 28: 50-61 -
Hodges, R. S. et al. (1988) Synthetic peptides define linear antigenic determinants recognized by monoclonal antibodies directed to the cytoplasmic carboxyl terminus of rhodopsin.
J Biol Chem 263: 11768-75 -
Molday, L. et al. (1990) The cGMP-gated cation channel of bovine rod photoreceptor cells is associated with a 240-kDa protein exhibiting immunochemical cross-reactivity with spectrin.
J Biol Chem. 265:18690-5.
- RRID
- AB_1833784
- UniProt
- P02699
- Entrez Gene
- RHO
- GO Terms
- GO:0005886 plasma membrane
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0004930 G-protein coupled receptor activity
- GO:0046872 metal ion binding
- GO:0007601 visual perception
- GO:0007602 phototransduction
- GO:0009881 photoreceptor activity
- GO:0018298 protein-chromophore linkage
- View More GO Terms
- GO:0042622 photoreceptor outer segment membrane
- GO:0060342 photoreceptor inner segment membrane
MCA4746
200815
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