Rhodopsin antibody | 1D4

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Mouse anti Rhodopsin

Product Type
Monoclonal Antibody
Clone
1D4
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA4746 IP P WB 0.1 ml
Mouse anti Rhodopsin antibody, clone 1D4 recognizes the sequence TETSQVAPA corresponding to aa 340 to 348 of bovine rhodopsin, a pigment of the retina responsible for the formation of photoreceptor cells and the first events in the perception of light. Rhodopsin consists of the protein opsin and a reversibly bound cofactor, retinal, a photoreactive chromophore. Upon light absorption the 11-cis-retinal is isomerised to all-trans retinal, leading to a change in the shape of rhodopsin. The change activates the associated G protein and triggers a second messenger cascade, eventually resulting in the transmission of a nerve impulse to the brain. The trans-retinal is released and converted back into 11-cis-retinal for re-entry into the cycle.

Defects in the gene cause various retinopathies such as retinitis pigmentosa and X-linked congenital stationary night blindness.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mammals
Amphibia
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
10mM HEPES pH7.5, 150mM NaCl
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.01% Bovine Serum Albumin
50% Glycerol
Immunogen
Bovine rhodopsin.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P02699 Related reagents
Entrez Gene
RHO Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0004930 G-protein coupled receptor activity
GO:0046872 metal ion binding
GO:0007601 visual perception
GO:0007602 phototransduction
GO:0009881 photoreceptor activity
GO:0018298 protein-chromophore linkage
GO:0042622 photoreceptor outer segment membrane
GO:0060342 photoreceptor inner segment membrane
Regulatory
For research purposes only

Applications of Rhodopsin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/100
Immunoprecipitation
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Mouse retina

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for Rhodopsin antibody

  1. Molday, R. S. et al. (1983) Monoclonal antibodies to rhodopsin: characterization, cross-reactivity, and application as structural probes.
    Biochemistry 22: 653-60
  2. MacKenzie, D. et al. (1984) Localization of binding sites for carboxyl terminal specific anti-rhodopsin monoclonal antibodies using synthetic peptides.
    Biochemistry 23: 6544-9
  3. Molday, R. S. et al. (1987) Peripherin. A rim-specific membrane protein of rod outer segment discs.
    Invest Ophthalmol Vis Sci. 28: 50-61
  4. Hodges, R. S. et al. (1988) Synthetic peptides define linear antigenic determinants recognized by monoclonal antibodies directed to the cytoplasmic carboxyl terminus of rhodopsin.
    J Biol Chem 263: 11768-75
  5. Molday, L. et al. (1990) The cGMP-gated cation channel of bovine rod photoreceptor cells is associated with a 240-kDa protein exhibiting immunochemical cross-reactivity with spectrin.
    J Biol Chem. 265:18690-5.