PI-3 Kinase p85 Subunit Alpha antibody | U13

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Mouse anti PI-3 Kinase p85 Subunit Alpha

Product Type
Monoclonal Antibody
Clone
U13
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1168 IP WB 2 ml
Mouse anti PI-3 Kinase p85 Subunit alpha antibody, clone U13 recognizes the SH3 domain of the p85 alpha subunit of bovine PI3 kinase, also known as Phosphatidylinositol 3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha or PtdIns-3-kinase regulatory subunit p85-alpha. p85 α PI3 kinase is a 724 amino acid ~85 kDa protein bearing a single Rho-GAP domain, two SH2 domains and a single N-terminal SH3 domain. Multiple isoforms of the protein can be generated by alternative splicing, mouse anti PI-3 Kinase p85 subunit alpha antibody, clone U13 recognizes only those isoforms containing the SH3 domain (isoforms 1 and 4) but not those lacking the domain (isoforms 2,3 and 5).

Deficiencies in the expression of the PIK3R1 gene can lead to immunodeficiency Agammaglobulinemia 7, autosomal recessive, diue to the inhibition of B cell development (Conley et al. 2012) and the develpmental condition SHORT syndrome (Chudasama et al. 2013).

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Monkey
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preparation
Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Balculovirus expressed (Recombinant) Bovine p85 alpha subunit of PI3 kinase.
Fusion Partners
Spleen cells from immunized mice were fused with cells of the NS1 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P27986 Related reagents
P23727 Related reagents
P26450 Related reagents
Q63787 Related reagents
Entrez Gene
PIK3R1 Related reagents
Pik3r1 Related reagents
PIK3R1 Related reagents
Pik3r1 Related reagents
GO Terms
GO:0043125 ErbB-3 class receptor binding
GO:0005158 insulin receptor binding
GO:0005159 insulin-like growth factor receptor binding
GO:0005943 1-phosphatidylinositol-4-phosphate 3-kinase, class IA complex
GO:0008286 insulin receptor signaling pathway
GO:0046326 positive regulation of glucose import
GO:0035014 phosphatidylinositol 3-kinase regulator activity
GO:0043560 insulin receptor substrate binding
GO:0046854 phosphatidylinositol phosphorylation
GO:0048009 insulin-like growth factor receptor signaling pathway
GO:0090004 positive regulation of establishment of protein localization in plasma membrane
GO:0001953 negative regulation of cell-matrix adhesion
GO:0006468 protein phosphorylation
GO:0016303 1-phosphatidylinositol-3-kinase activity
GO:0030183 B cell differentiation
GO:0030335 positive regulation of cell migration
GO:0043066 negative regulation of apoptosis
GO:0045671 negative regulation of osteoclast differentiation
GO:0005545 1-phosphatidylinositol binding
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0014065 phosphatidylinositol 3-kinase cascade
GO:0019903 protein phosphatase binding
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030168 platelet activation
GO:0031295 T cell costimulation
GO:0043559 insulin binding
GO:0044419 interspecies interaction between organisms
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050852 T cell receptor signaling pathway
GO:0050900 leukocyte migration
GO:0060396 growth hormone receptor signaling pathway
GO:0005886 plasma membrane
GO:0001878 response to yeast
GO:0001934 positive regulation of protein phosphorylation
GO:0005161 platelet-derived growth factor receptor binding
GO:0005516 calmodulin binding
GO:0005625 soluble fraction
GO:0005634 nucleus
GO:0006006 glucose metabolic process
GO:0030971 receptor tyrosine kinase binding
GO:0007584 response to nutrient
GO:0008022 protein C-terminus binding
GO:0042493 response to drug
GO:0009750 response to fructose stimulus
GO:0010040 response to iron(II) ion
GO:0010459 negative regulation of heart rate
GO:0010628 positive regulation of gene expression
GO:0046935 1-phosphatidylinositol-3-kinase regulator activity
GO:0019717 synaptosome
GO:0019901 protein kinase binding
GO:0019904 protein domain specific binding
GO:0022408 negative regulation of cell-cell adhesion
GO:0030331 estrogen receptor binding
GO:0031625 ubiquitin protein ligase binding
GO:0032355 response to estradiol stimulus
GO:0032570 response to progesterone stimulus
GO:0033574 response to testosterone stimulus
GO:0043200 response to amino acid stimulus
GO:0043548 phosphatidylinositol 3-kinase binding
GO:0043551 regulation of phosphatidylinositol 3-kinase activity
GO:0045471 response to ethanol
GO:0045663 positive regulation of myoblast differentiation
GO:0045768 positive regulation of anti-apoptosis
GO:0045776 negative regulation of blood pressure
GO:0045861 negative regulation of proteolysis
GO:0048662 negative regulation of smooth muscle cell proliferation
GO:0051117 ATPase binding
GO:0051219 phosphoprotein binding
GO:0051591 response to cAMP
GO:0070848 response to growth factor stimulus
GO:0071398 cellular response to fatty acid
GO:0071445 cellular response to protein stimulus
GO:0071548 response to dexamethasone stimulus
Regulatory
For research purposes only

Applications of PI-3 Kinase p85 Subunit Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation Neat
Western Blotting 1/200
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Immunoprecipitation

Western Blotting

Product Specific References

References for PI-3 Kinase p85 Subunit Alpha antibody

  1. Woodfield, R.J. et al. (2001) The p85 subunit of phosphoinositide 3-kinase is associated with beta-catenin in the cadherin-based adhesion complex.
    Biochem J. 360 (Pt 2): 335-44.
  2. Reif, K. et al. (1993) Divergent regulation of phosphatidylinositol 3-kinase P85 alpha and P85 beta isoforms upon T cell activation.
    J Biol Chem. 268 (15): 10780-8.
  3. Dawson, C.W. et al. (2003) Epstein-Barr virus latent membrane protein 1 (LMP1) activates the phosphatidylinositol 3-kinase/Akt pathway to promote cell survival and induce actin filament remodeling.
    J Biol Chem. 278: 3694-704.
  4. Cross, M.J. et al. (1995) Wortmannin and its structural analogue demethoxyviridin inhibit stimulated phospholipase A2 activity in Swiss 3T3 cells. Wortmannin is not a specific inhibitor of phosphatidylinositol 3-kinase.
    J Biol Chem. 270: 25352-5.
  5. Gillham, H. et al. (1999) Intracellular movement of green fluorescent protein-tagged phosphatidylinositol 3-kinase in response to growth factor receptor signaling.
    J Cell Biol. 146: 869-80.
  6. Shepherd, P.R. et al. (1997) Differential regulation of phosphoinositide 3-kinase adapter subunit variants by insulin in human skeletal muscle.
    J Biol Chem. 272: 19000-7.
  7. Raymond, A.A. et al. (2017) Reptin regulates insulin-stimulated Akt phosphorylation in hepatocellular carcinoma via the regulation of SHP-1/PTPN6.
    Cell Biochem Funct. 35 (6): 289-95.