Myelin Proteolipid Protein antibody | AbD05702

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Human anti Myelin Proteolipid Protein

Product Type
Monoclonal Antibody
Clone
AbD05702
Isotype
HuCAL Fab bivalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA095A E WB 50 µg
HCA095 E WB 0.1 mg
Human anti myelin proteolipid protein antibody, clone AbD05702 recognizes myelin proteolipid protein (PLP), which is a major constituent protein of central nervous system myelin. The Plp gene is highly conserved across species and encodes two major membrane-bound proteins of the myelin sheath, the large isoform PLP and a smaller isoform DM20. The exact functions of PLP are still unclear but reports suggest that PLP and DM20 may play a role in the formation of the intraperiod line of myelin and in maintaining axonal integrity.

In man, mutations of the PLP and DM20 proteins have been reported which are associated with X-linked dysmyelinating disorders including the heterogeneous syndromes of Pelizaeus-Merzbacher disease (PMD). The reported mutations vary considerably and range from deletions, missense mutations to additional copies of the gene.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rabbit Expected from Sequence
Dog Expected from Sequence
Mouse Expected from Sequence
Rat Expected from Sequence
Pig Expected from Sequence
Human Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilized.
Reconstitution
Pack Size: 50 µg
Reconstitute with 0.5 ml distilled water
Pack Size: 0.1 mg
Reconstitute with 1.0 ml distilled water
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Synthetic peptide corresponding to the sequence CGKGLSATVTGGQKGRGSR of human myelin proteolipid protein 1
Approx. Protein Concentrations
Pack Size: 50 µg
Total protein concentration 0.1 mg/ml
Pack Size: 0.1 mg
Ig concentration 0.1 mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution

More Information

UniProt
P60201 Related reagents
Entrez Gene
PLP1 Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0007268 synaptic transmission
GO:0008219 cell death
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of Myelin Proteolipid Protein antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1/100 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Histidine Tag:Alk.Phos. MCA1396A 0.1 mg E WB
Mouse anti Histidine Tag:Biotin MCA1396B 0.1 mg E WB
Mouse anti c-Myc:Alk.Phos. MCA2200A 0.1 mg C E P WB*
Mouse anti c-Myc:Biotin MCA2200B 0.1 mg C E P WB*
Mouse anti c-Myc:HRP MCA2200P 0.1 mg C E P WB*
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB

Product Specific References

References for Myelin Proteolipid Protein antibody

  1. Weigel, I. et al. (2010) Immunochemical Detection of Tissue from the Central Nervous System via Myelin Proteolipid Protein: Adaptation for Food Inspection and Development of Recombinant Bivalent Fab Mini-Antibodies.
    J Agric Food Chem. 58: 6587-93.