MHC Class II DR antibody | CC108

Mouse anti Bovine MHC Class II DR:RPE

Product Type
Monoclonal Antibody
Clone
CC108
Isotype
IgG1
Specificity
MHC Class II DR

Product Code Applications Pack Size List Price Your Price Qty
MCA5656PE
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Mouse anti Bovine MHC class II DR antibody, clone CC108 recognizes Bovine MHC Class II DR. MHC Class II molecules are constitutively expressed on antigen presenting cells such as dendritic cells, B lymphocytes, monocytes, macrophages, activated T lymphocytes and may be induced on a range of other cell types by interferon gamma.

The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In cattle, this complex is referred to as the bovine leukocyte antigen (BoLA) region. There are 2 major types of MHC class IIa molecules encoded by the BoLA which are DR and DQ each composed of an alpha and beta chain.

Target Species
Bovine
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the Mouse NS1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader
List Price Your Price
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Description Mouse IgG1 Negative Control:RPE

References for MHC Class II DR antibody

  1. Stephens, S.A. & Howard, C.J. (2002) Infection and transformation of dendritic cells from bovine afferent lymph by Theileria annulata.
    Parasitology. 124 (Pt 5): 485-93.
  2. Yamakawa, Y. et al. (2008) Identification and functional characterization of a bovine orthologue to DC-SIGN.
    J Leukoc Biol. 83 (6): 1396-403.
  3. Corripio-Miyar, Y. et al. (2015) Phenotypic and functional analysis of monocyte populations in cattle peripheral blood identifies a subset with high endocytic and allogeneic T-cell stimulatory capacity.
    Vet Res. 46: 112.
  4. Guzman, E. et al. (2014) Bovine γδ T cells are a major regulatory T cell subset.
    J Immunol. 193 (1): 208-22.
  5. Childerstone, A.J. et al. (1999) Demonstration of bovine CD8+ T-cell responses to foot-and-mouth disease virus.
    J Gen Virol. 80 ( Pt 3): 663-9.
  6. Sopp, P. et al. (1994) Detection of bovine viral diarrhoea virus p80 protein in subpopulations of bovine leukocytes.
    J Gen Virol. 75 ( Pt 5): 1189-94.
  7. Bembridge, G.P. et al. (1995) CD45RO expression on bovine T cells: relation to biological function.
    Immunology. 86 (4): 537-44.
  8. Gibson, A.J. et al. (2016) Differential macrophage function in Brown Swiss and Holstein Friesian cattle.
    Vet Immunol Immunopathol. 181: 15-23.
  9. View The Latest Product References
  10. Corripio-miyar, Y. et al. (2017) 1,25-Dihydroxyvitamin D3 modulates the phenotype and function of Monocyte derived dendritic cells in cattle
    BMC Veterinary Research. 13 (1) [Epub ahead of print].
  11. Risalde, M.A. et al. (2020) BVDV permissiveness and lack of expression of co-stimulatory molecules on PBMCs from calves pre-infected with BVDV.
    Comp Immunol Microbiol Infect Dis. 68: 101388.
  12. Park, D.S. et al. (2021) Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress.
    Cell Stress Chaperones. 26 (4): 705-20.

RRID
AB_11152779
UniProt
Q30309
P79464
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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