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MBP antibody | 2

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Mouse anti MBP (aa119-131)

Product Type
Monoclonal Antibody
Clone
2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA184S C E 2 ml
Mouse anti MBP antibody, clone 2 recognizes bovine myelin basic protein, also known as 20 kDa microtubule-stabilizing protein. MBP is a 169 amino acid component of the myelin membrane. Mouse anti MBP antibody, clone 2 binds to an epitope located in the region of residues 119-131 (GAEGQRPGFGYGG) .

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rabbit
Rat
Sheep
Pig
Human
Guinea Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preparation
Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Bovine myelin basic protein.
Fusion Partners
Spleen cells from immunised NIH/OIa mice were fused with cells of the NS0 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P02687 Related reagents
Entrez Gene
MBP Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0019911 structural constituent of myelin sheath
GO:0043209 myelin sheath
Regulatory
For research purposes only

Applications of MBP antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Clone 2 has also been reported to work in Western Blotting.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for MBP antibody

  1. Groome, N. et al. (1985) Preparation and properties of monoclonal antibodies to myelin basic protein and its peptides.
    Neurochem Int. 7 (2): 309-17.
  2. Martensen, R.W. (1984) .A useful model for multiple sclerosis
    (in Experimental Allergic Encephalomyelitis Alvord E.C., Kies, M.W. and Suckling, A.J. eds). pp 511-521 Alan Liss N.Y.
  3. Groome, N.P. et al. (1986) Region-specific immunoassays for human myelin basic protein.
    J Neuroimmunol. 12 (4): 253-64.
  4. Scherer, S.S. et al. (1995) Periaxin expression in myelinating Schwann cells: modulation by axon-glial interactions and polarized localization during development
    Development. 121: 4265-73.
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