MBP antibody | 2
Mouse anti MBP antibody, clone 2 recognizes bovine myelin basic protein, also known as 20 kDa microtubule-stabilizing protein. MBP is a 169 amino acid component of the myelin membrane. Mouse anti MBP antibody, clone 2 binds to an epitope located in the region of residues 119-131 (GAEGQRPGFGYGG) .
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Rabbit Rat Sheep Pig Human Guinea Pig
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue Culture Supernatant - liquid
- Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
- Preservative Stabilisers
0.1% Sodium Azide
- Bovine myelin basic protein.
- Fusion Partners
- Spleen cells from immunised NIH/OIa mice were fused with cells of the NS0 mouse myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- P02687 Related reagents
- Entrez Gene
- MBP Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0005515 protein binding
- GO:0019911 structural constituent of myelin sheath
- GO:0043209 myelin sheath
- For research purposes only
Applications of MBP antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Clone 2 has also been reported to work in Western Blotting.
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Secondary Antibodies Available
Product Specific References
References for MBP antibody
Groome, N. et al. (1985) Preparation and properties of monoclonal antibodies to myelin basic protein and its peptides.
Neurochem Int. 7 (2): 309-17.
Martensen, R.W. (1984) .A useful model for multiple sclerosis
(in Experimental Allergic Encephalomyelitis Alvord E.C., Kies, M.W. and Suckling, A.J. eds). pp 511-521 Alan Liss N.Y.
Groome, N.P. et al. (1986) Region-specific immunoassays for human myelin basic protein.
J Neuroimmunol. 12 (4): 253-64.
Scherer, S.S. et al. (1995) Periaxin expression in myelinating Schwann cells: modulation by axon-glial interactions and polarized localization during development
Development. 121: 4265-73.