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IgG2 antibody | IL-A73

Product Code Applications Pack Size List Price Your Price Qty
MCA2441GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F IP 0.1 mg loader
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loader

Mouse anti Bovine IgG2, clone IL-A73, is a monoclonal antibody specific for bovine IgG2 and does not recognise other bovine immunoglobulin classes.

While normal serum levels of IgG1 are relatively constant in cattle at around 50%, IgG2 levels are highly variable between different strains and races.

Mouse anti Bovine IgG2, clone IL-A73 immunoprecipitates a protein band of approximately 52-59kDa, consistent with the heavy chain of bovine IgG2 (Campbell et al. 1998).

Target Species
Bovine
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Purified bovine IgG2
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/20 000 1/200 000
Flow Cytometry
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

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References for IgG2 antibody

  1. Campbell, J.D. et al. (1998) A novel cell surface proliferation-associated marker expressed on T cells and up-regulated on germinal center B cells.
    J Leukoc Biol. 63 (5): 567-74.
  2. Williams, D.J. et al. (1990) Quantitation of bovine immunoglobulin isotypes and allotypes using monoclonal antibodies.
    Vet Immunol Immunopathol. 24 (3): 267-83.
  3. Williams, D.J. et al. (1996) The role of anti-variable surface glycoprotein antibody responses in bovine trypanotolerance.
    Parasite Immunol. 18 (4): 209-18.
  4. Hecker, Y.P. et al. (2014) A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge.
    Vet Immunol Immunopathol. 162 (3-4): 142-53.
  5. Dorneles, E.M. et al. (2015) Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
    PLoS One. 10 (9): e0136696.
  6. Pereyra, R. et al. (2019) Evidence of reduced vertical transmission of Neospora caninum. associated with higher IgG1 than IgG2 serum levels and presence of IFN-γ in non-aborting chronically infected cattle under natural condition.
    Vet Immunol Immunopathol. 208: 53-57.
  7. Jaramillo, J.O. et al. (2019) Immunisation of cattle against Babesia bovis. combining a multi-epitope modified vaccinia Ankara virus and a recombinant protein induce strong Th1 cell responses but fails to trigger neutralising antibodies required for protection.
    Ticks Tick Borne Dis. 10 (6): 101270.
  8. Villa-Mancera, A. et al. (2021) Phage display-based vaccine with cathepsin L and excretory-secretory products mimotopes of Fasciola hepatica. induces protective cellular and humoral immune responses in sheep.
    Vet Parasitol. 289: 109340.
  9. View The Latest Product References
  10. Di Giacomo, S. et al. (2022) Assessment on Different Vaccine Formulation Parameters in the Protection against Heterologous Challenge with FMDV in Cattle.
    Viruses. 14 (8): 1781.

MCA2441GA

1801

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