IgA antibody | IL-A71
Mouse anti Bovine IgA
- Product Type
- Monoclonal Antibody
- Clone
- IL-A71
- Isotype
- IgG2a
- Specificity
- IgA
Antibody Quality and Performance Guarantee
| Mouse anti Bovine IgA, clone IL-A71, is specific for bovine immunoglobulin A (IgA). IgA is the predominant surface secreted immunoglobulin from the intestinal, urogenital and respiratory tracts. IgA functions in a protective capacity, primarily, by preventing viruses and bacteria from adhering to epithelial tissues. High concentrations of IgA are found in saliva, colostrum, milk, tear duct fluid, urine and nasal fluid. Clone IL-A71 is reported to immunoprecipitate bands of approximately 62kDa and 75kDa, which is consistent with the heavy chain and the secretory component of IgA (Williams et al. 1990). In addition to clone IL-A71 which is specific for bovine IgA, monoclonal antibodies recognizing the other major bovine immunoglobulin classes and sub-classes are also available from Bio-Rad. |
- Target Species
- Bovine
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Purified bovine Immunoglobulin
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/50 | 1/5000 |
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Bovine IgG | MCA2439GA | E IP | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse anti Bovine IgG | ||||||
| Mouse anti Bovine IgG1 | MCA2440GA | C E F IP | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse anti Bovine IgG1 | ||||||
| Mouse anti Bovine IgG2 | MCA2441GA | E F IP | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse anti Bovine IgG2 | ||||||
| Mouse anti Bovine IgM | MCA2443GA | E F IP R | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse anti Bovine IgM | ||||||
| Mouse anti Sheep IgE | MCA5941GA | C E IF WB | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse anti Sheep IgE | ||||||
References for IgA antibody
-
Williams, D.J. et al. (1990) Quantitation of bovine immunoglobulin isotypes and allotypes using monoclonal antibodies.
Vet Immunol Immunopathol. 24 (3): 267-83. -
van Dissel, J.T. et al. (2005) Bovine antibody-enriched whey to aid in the prevention of a relapse of Clostridium difficile-associated diarrhoea: preclinical and preliminary clinical data.
J Med Microbiol. 54 (Pt 2): 197-205. -
Sridevi, N.V. et al. (2014) Development of anti-bovine IgA single chain variable fragment and its application in diagnosis of foot-and-mouth disease.
Eur J Microbiol Immunol (Bp). 4 (1): 34-44. -
Hägglund, S. et al. (2011) Bovine respiratory syncytial virus ISCOMs-Immunity, protection and safety in young conventional calves.
Vaccine. 29 (47): 8719-30. -
Mwirigi, M. et al. (2016) Capsular polysaccharide from Mycoplasma mycoides subsp. mycoides shows potential for protection against contagious bovine pleuropneumonia.
Vet Immunol Immunopathol. 178: 64-9. -
Blodörn, K. et al. (2014) Vaccine safety and efficacy evaluation of a recombinant bovine respiratory syncytial virus (BRSV) with deletion of the SH gene and subunit vaccines based on recombinant human RSV proteins: N-nanorings, P and M2-1, in calves with maternal antibodies.
PLoS One. 9 (6): e100392. -
Ribeiro, L.E. et al. (2021) Selection and characterization of peptides mimetic to Campylobacter fetus. subsp. venerealis. using phage display.
Ciêia Rural 51 (8) [Epub ahead of print].
MCA2438GA
0514
163359
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with IgA specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Bovine ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up