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IgA antibody | IL-A71

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Bovine IgA detected in bovine serum using Mouse anti Bovine IgA (MCA2438GA) followed by Goat anti Mouse IgG:HRP (STAR77).

Mouse anti Bovine IgA:HRP

Product Type
Monoclonal Antibody
Clone
IL-A71
Isotype
IgG2a
Specificity
IgA

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Product Code Applications Pack Size List Price Your Price Qty
MCA2438P
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.25 mg loader
List Price Your Price
loader

Mouse anti Bovine IgA, clone IL-A71, is specific for bovine immunoglobulin A (IgA).

IgA is the predominant surface secreted immunoglobulin from the intestinal, urogenital and respiratory tracts. IgA functions in a protective capacity, primarily, by preventing viruses and bacteria from adhering to epithelial tissues. High concentrations of IgA are found in saliva, colostrum, milk, tear duct fluid, urine and nasal fluid.

Clone IL-A71 is reported to immunoprecipitate bands of approximately 62kDa and 75kDa, which is consistent with the heavy chain and the secretory component of IgA (Williams et al. 1990).

In addition to clone IL-A71 which is specific for bovine IgA, monoclonal antibodies recognizing the other major bovine immunoglobulin classes and sub-classes are also available from Bio-Rad.

Target Species
Bovine
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
Immunogen
Purified bovine Immunoglobulin
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus

References for IgA antibody

  1. Williams, D.J. et al. (1990) Quantitation of bovine immunoglobulin isotypes and allotypes using monoclonal antibodies.
    Vet Immunol Immunopathol. 24 (3): 267-83.
  2. van Dissel, J.T. et al. (2005) Bovine antibody-enriched whey to aid in the prevention of a relapse of Clostridium difficile-associated diarrhoea: preclinical and preliminary clinical data.
    J Med Microbiol. 54 (Pt 2): 197-205.
  3. Sridevi, N.V. et al. (2014) Development of anti-bovine IgA single chain variable fragment and its application in diagnosis of foot-and-mouth disease.
    Eur J Microbiol Immunol (Bp). 4 (1): 34-44.
  4. Hägglund, S. et al. (2011) Bovine respiratory syncytial virus ISCOMs-Immunity, protection and safety in young conventional calves.
    Vaccine. 29 (47): 8719-30.
  5. Mwirigi, M. et al. (2016) Capsular polysaccharide from Mycoplasma mycoides subsp. mycoides shows potential for protection against contagious bovine pleuropneumonia.
    Vet Immunol Immunopathol. 178: 64-9.
  6. Blodörn, K. et al. (2014) Vaccine safety and efficacy evaluation of a recombinant bovine respiratory syncytial virus (BRSV) with deletion of the SH gene and subunit vaccines based on recombinant human RSV proteins: N-nanorings, P and M2-1, in calves with maternal antibodies.
    PLoS One. 9 (6): e100392.
  7. Ribeiro, L.E. et al. (2021) Selection and characterization of peptides mimetic to Campylobacter fetus. subsp. venerealis. using phage display.
    Ciêia Rural 51 (8) [Epub ahead of print].

MCA2438P

159422 168152 1705

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