IFN Gamma antibody | 5D10

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Mouse anti Bovine Interferon Gamma

Product Type: Monoclonal Antibody
Clone: 5D10
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2689GA	E ES			0.1 mg

Mouse anti Bovine interferon gamma antibody, clone 5D10, recognises IFNγ, a 19.3 kDa protein belonging to the type II interferon family .

Bovine IFNγ is produced by activated lymphocytes, has anti-viral and immunoregulatory properties and is an activator of macrophages. Clone 5D10 is one of a range of bovine IFNγ reagents available from Bio-Rad which include the monoclonal antibody clones CC330 (MCA2112), CC302 (MCA1783) and 7B6 (MCA1964) along with recombinant bovine IFNγ and bovine IFNγ ELISA kits.

Mouse anti Bovine interferon gamma antibody, clone 5D10 is suitable for ELISA and ELISpot, for intracellular identification of bovine IFNγ and may be used in conjuction with clone CC302 (clone CC302 is available in a range of direct conjugates) as a detection reagent.

Product Details

Target Species: Bovine
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Carrier Free: Yes
Immunogen: Purified recombinant bovine IFN gamma expressed in E.coli.
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the Sp20/Ag-14 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P07353 Related reagents
Entrez Gene: IFNG Related reagents
GO Terms: GO:0009615 response to virus; GO:0005125 cytokine activity; GO:0005133 interferon-gamma receptor binding; GO:0005615 extracellular space; GO:0006955 immune response
Regulatory: For research purposes only

Applications of IFN Gamma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
ELISpot

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

ELISA: This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with MCA1783B as the detection reagent. PBP007A may be used as a standard.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Mouse anti Bovine Interferon Gamma:Alexa Fluor®488	MCA1783A488	100 Tests/1ml	F*
Mouse anti Bovine Interferon Gamma:Alexa Fluor®647	MCA1783A647	100 Tests/1ml	F*
Mouse anti Bovine Interferon Gamma:Biotin	MCA1783B	0.25 mg	E
Mouse anti Bovine Interferon Gamma:FITC	MCA1783F	0.1 mg	F*
Mouse anti Bovine Interferon Gamma:RPE	MCA1783PE	100 Tests	F*
Mouse anti Bovine Interferon Gamma	MCA1964	0.5 mg	F* P* WB
Mouse anti Bovine Interferon Gamma	MCA2112	0.5 mg	E ES F*
Bovine Interferon Gamma ELISA Kit	MCA5638KZZ	5 X 96 Wells	E

Product Specific References

References for IFN Gamma antibody

Walravens, K. et al. (2002) Analysis of the antigen-specific IFN-gamma producing T-cell subsets in cattle experimentally infected with Mycobacterium bovis.
Vet Immunol Immunopathol. 84 (1-2): 29-41.
Lemaire, M. et al. (2000) Effects of bovine herpesvirus type 1 infection in calves with maternal antibodies on immune response and virus latency.
J Clin Microbiol. 38 (5): 1885-94.
Kennedy, H.E. et al. (2002) Modulation of immune responses to Mycobacterium bovis in cattle depleted of WC1(+) gamma delta T cells.
Infect Immun. 70 (3): 1488-500.
Weynants, V. et al. (1998) Quantitative assessment by flow cytometry of T-lymphocytes producing antigen-specific gamma-interferon in Brucella immune cattle.
Vet Immunol Immunopathol. 66 (3-4): 309-20.

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