IFN Gamma antibody | 5D10

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Mouse anti Bovine Interferon Gamma

Product Type
Monoclonal Antibody
Clone
5D10
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2689GA E ES 0.1 mg
Mouse anti Bovine interferon gamma antibody, clone 5D10, recognises IFNγ, a 19.3 kDa protein belonging to the type II interferon family .

Bovine IFNγ is produced by activated lymphocytes, has anti-viral and immunoregulatory properties and is an activator of macrophages. Clone 5D10 is one of a range of bovine IFNγ reagents available from Bio-Rad which include the monoclonal antibody clones CC330 (MCA2112), CC302 (MCA1783) and 7B6 (MCA1964) along with recombinant bovine IFNγ and bovine IFNγ ELISA kits.

Mouse anti Bovine interferon gamma antibody, clone 5D10 is suitable for ELISA and ELISpot, for intracellular identification of bovine IFNγ and may be used in conjuction with clone CC302 (clone CC302 is available in a range of direct conjugates) as a detection reagent.

Product Details

Target Species
Bovine
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Purified recombinant bovine IFN gamma expressed in E.coli.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the Sp20/Ag-14 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P07353 Related reagents
Entrez Gene
IFNG Related reagents
GO Terms
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005133 interferon-gamma receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
Regulatory
For research purposes only

Applications of IFN Gamma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
ELISpot
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with MCA1783B as the detection reagent. PBP007A may be used as a standard.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Bovine Interferon Gamma:Alexa Fluor®488 MCA1783A488 100 Tests/1ml F*
Mouse anti Bovine Interferon Gamma:Alexa Fluor®647 MCA1783A647 100 Tests/1ml F*
Mouse anti Bovine Interferon Gamma:Biotin MCA1783B 0.25 mg E
Mouse anti Bovine Interferon Gamma:FITC MCA1783F 0.1 mg F*
Mouse anti Bovine Interferon Gamma:RPE MCA1783PE 100 Tests F*
Mouse anti Bovine Interferon Gamma MCA1964 0.5 mg F* P* WB
Mouse anti Bovine Interferon Gamma MCA2112 0.5 mg E ES F*
Bovine Interferon Gamma ELISA Kit MCA5638KZZ 5 X 96 Wells E

Product Specific References

References for IFN Gamma antibody

  1. Walravens, K. et al. (2002) Analysis of the antigen-specific IFN-gamma producing T-cell subsets in cattle experimentally infected with Mycobacterium bovis.
    Vet Immunol Immunopathol. 84 (1-2): 29-41.
  2. Lemaire, M. et al. (2000) Effects of bovine herpesvirus type 1 infection in calves with maternal antibodies on immune response and virus latency.
    J Clin Microbiol. 38 (5): 1885-94.
  3. Kennedy, H.E. et al. (2002) Modulation of immune responses to Mycobacterium bovis in cattle depleted of WC1(+) gamma delta T cells.
    Infect Immun. 70 (3): 1488-500.
  4. Weynants, V. et al. (1998) Quantitative assessment by flow cytometry of T-lymphocytes producing antigen-specific gamma-interferon in Brucella immune cattle.
    Vet Immunol Immunopathol. 66 (3-4): 309-20.