GM-CSF antibody | CC305
Recombinant Bovine GM-CSF detected using Mouse anti Bovine GM-CSF (MCA1828GA) as the capture reagent and a bovine reactive Mouse anti Sheep GM-CSF, clone 3C2 (MCA1924) conjugated to Biotin as the detection reagent followed by Streptavidin:HRP
Mouse anti Bovine GM-CSF antibody, clone CC305 recognizes bovine granulocyte macrophage colony-stimulating factor (GM-CSF) also known as CSF-2. Bovine GM-CSF is a 143 amino acid cytokine which stimulates proliferation and differentiation of hematopoietic precursors.
Mouse anti Bovine GM-CSF antibody, clone CC305 has been used as a capture antibody in conjunction with Mouse anti Sheep GM-CSF, clone 3C2 as a detection reagent in a sandwich ELISA for the measurement of bovine GM-CSF produced by peripheral blood mononuclear cells in vitro (Kennedy et al. 2002).
Mouse anti Bovine GM-CSF antibody, clone CC305 has been used as a capture antibody in conjunction with Mouse anti Sheep GM-CSF, clone 3C2 as a detection reagent in a sandwich ELISA for the measurement of bovine GM-CSF produced by peripheral blood mononuclear cells in vitro (Kennedy et al. 2002).
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised C57/BL mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P11052
- Entrez Gene
- CSF2
- GO Terms
- GO:0005125 cytokine activity
- GO:0005129 granulocyte macrophage colony-stimulating factor receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- Regulatory
- For research purposes only
Applications of GM-CSF antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 5 ug/ml as coating antibody | ||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This product may be used as a capture antibody in a sandwich ELISA together with MCA1924 when conjugated to biotin as the detection reagent.
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Secondary Antibodies Available
Product Specific References
References for GM-CSF antibody
-
Kennedy HE et al. (2002) Modulation of immune responses to Mycobacterium bovis in cattle depleted of WC1(+) gamma delta T cells.
Infect Immun. 70 (3): 1488-500. -
Boutet, P. et al. (2004) Delayed neutrophil apoptosis in bovine subclinical mastitis.
J Dairy Sci. 87 (12): 4104-14. -
Bao, Z.J. et al. (2014) Recombinant bovine interferon-τ enhances in vitro development of bovine embryos by upregulating expression of connexin 43 and E-cadherin.
J Dairy Sci. 97 (11): 6917-25. -
Pedersen, L.G. et al. (2002) Identification of monoclonal antibodies that cross-react with cytokines from different animal species
Vet Immunol Immunopathol. 88: 111-22. -
Davies, C.J. et al. (2004) Methods of minimizing immunological rejection of a nuclear transfer fetus.
U.S. Patent Application: US 2004/0029825 A1
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