GM-CSF antibody | CC305

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Mouse anti Bovine GM-CSF

Product Type
Monoclonal Antibody
Clone
CC305
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1828GA E WB 0.1 mg
Mouse anti Bovine GM-CSF antibody, clone CC305 recognizes bovine granulocyte macrophage colony-stimulating factor (GM-CSF) also known as CSF-2. Bovine GM-CSF is a 143 amino acid cytokine which stimulates proliferation and differentiation of hematopoietic precursors.

Mouse anti Bovine GM-CSF antibody, clone CC305 has been used as a capture antibody in conjunction with Mouse anti Sheep GM-CSF, clone 3C2 as a detection reagent in a sandwich ELISA for the measurement of bovine GM-CSF produced by peripheral blood mononuclear cells in vitro (Kennedy et al. 2002).

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised C57/BL mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch

More Information

UniProt
P11052 Related reagents
Entrez Gene
CSF2 Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005129 granulocyte macrophage colony-stimulating factor receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
Regulatory
For research purposes only

Applications of GM-CSF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5 ug/ml as coating antibody
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used as a capture antibody in a sandwich ELISA together with MCA1924 when conjugated to biotin as the detection reagent.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for GM-CSF antibody

  1. Kennedy HE et al. (2002) Modulation of immune responses to Mycobacterium bovis in cattle depleted of WC1(+) gamma delta T cells.
    Infect Immun. 70 (3): 1488-500.
  2. Boutet, P. et al. (2004) Delayed neutrophil apoptosis in bovine subclinical mastitis.
    J Dairy Sci. 87 (12): 4104-14.
  3. Bao, Z.J. et al. (2014) Recombinant bovine interferon-τ enhances in vitro development of bovine embryos by upregulating expression of connexin 43 and E-cadherin.
    J Dairy Sci. 97 (11): 6917-25.
  4. Pedersen, L.G. et al. (2002) Identification of monoclonal antibodies that cross-react with cytokines from different animal species
    Vet Immunol Immunopathol. 88: 111-22.
  5. Davies, C.J. et al. (2004) Methods of minimizing immunological rejection of a nuclear transfer fetus.
    U.S. Patent Application: US 2004/0029825 A1