Dynein Cytoplasmic antibody | 74.1
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Mouse anti Bovine Dynein Cytoplasmic 74kDa
- Product Type
- Monoclonal Antibody
- Clone
- 74.1
- Isotype
- IgG2b
- Specificity
- Dynein Cytoplasmic
- Region
- 74kDa
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| Mouse anti Bovine dynein cytoplasmic 74 kDa antibody, clone 74.1> reacts with all known forms of the 74 kDa intermediate chain subunit of mammalian cytoplasmic dynein. The antigenic site has been localized to the first 60 amino acids of the N-terminal portion of the molecule. Mouse anti Bovine dynein cytoplasmic 74 kDa antibody, clone 74.1 immunoprecipitates the intermediate chain subunits under denaturing conditions and the entire dynein complex under native conditions. |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Fish Mouse Drosophila Xenopus Rat Sheep Invertebrates - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Native cytoplasmic dynein from bovine brain.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen |  |
1/10 | 1/100 |
| Immunoprecipitation | |
||
| Western Blotting | |
1/1000 | 1/5000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Cultured MDCK cells, NRR cells, N1E cells and PTK-1 cells.
- RRID
- AB_619952
- UniProt
- Q0III3
- Entrez Gene
- DYNC1I2
- GO Terms
- GO:0003774 motor activity
- GO:0005737 cytoplasm
- GO:0005874 microtubule
- GO:0006810 transport
- GO:0030286 dynein complex
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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