Dynein Cytoplasmic antibody | 74.1
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Mouse anti Bovine dynein cytoplasmic 74 kDa antibody, clone 74.1> reacts with all known forms of the 74 kDa intermediate chain subunit of mammalian cytoplasmic dynein. The antigenic site has been localized to the first 60 amino acids of the N-terminal portion of the molecule.
Mouse anti Bovine dynein cytoplasmic 74 kDa antibody, clone 74.1 immunoprecipitates the intermediate chain subunits under denaturing conditions and the entire dynein complex under native conditions.
Mouse anti Bovine dynein cytoplasmic 74 kDa antibody, clone 74.1 immunoprecipitates the intermediate chain subunits under denaturing conditions and the entire dynein complex under native conditions.
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Fish Mouse Drosophila Xenopus Rat Sheep Invertebrates - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Native cytoplasmic dynein from bovine brain.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- Q0III3 Related reagents
- Entrez Gene
- DYNC1I2 Related reagents
- GO Terms
- GO:0003774 motor activity
- GO:0005737 cytoplasm
- GO:0005874 microtubule
- GO:0006810 transport
- GO:0030286 dynein complex
- Regulatory
- For research purposes only
Applications of Dynein Cytoplasmic antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | 1/10 | 1/100 | |
| Immunoprecipitation | |||
| Western Blotting | 1/1000 | 1/5000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Cultured MDCK cells, NRR cells, N1E cells and PTK-1 cells.
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