CD5 antibody | B29A
A. Mouse IgG2a negative control (MCA929) followed by Goat anti Mouse IgG2a:PE (STAR133PE).
B. Mouse anti Bovine CD5 antibody, clone B29A (MCA6082) followed by Goat anti Mouse IgG2a:PE secondary antibody (STAR133PE).
Both the Mouse IgG2a negative control and Mouse anti Bovine CD5 are plotted against bovine CD40 as recognized by FITC conjugated Mouse anti Bovine CD40 antibody, clone IL-A156 (MCA2431F).
Mouse anti Bovine CD5, clone B29A, recognizes bovine CD5. The 67 kDa glycoprotein CD5 is present on alpha beta and gamma delta T cells but only on a small number of B cells. In contrast, in cattle infected with bovine leukemia virus almost all B cells were CD5+ (Depelchin et al. 1989). CD5 associates with CD2 and the CD3 zeta chain (Burgess et al. 1992).
Similarly to mouse or human, in cattle CD5 associates with the B cell receptor. However, in cattle infected with bovine leukemia virus CD5 dissociates from the B cell receptor (Cantor et al. 2001).
Similarly to mouse or human, in cattle CD5 associates with the B cell receptor. However, in cattle infected with bovine leukemia virus CD5 dissociates from the B cell receptor (Cantor et al. 2001).
Product Details
- Target Species
- Bovine
- Product Form
- Concentrated tissue culture supernatant - liquid
- Preparation
- Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
- Buffer Solution
- Serum free tissue culture medium containing proprietary protein free supplement
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Bovine lymphocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch
More Information
- UniProt
- P19238 Related reagents
- Entrez Gene
- CD5 Related reagents
- GO Terms
- GO:0016021 integral to membrane
- GO:0005044 scavenger receptor activity
- Regulatory
- For research purposes only
Applications of CD5 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Functional Assays |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG2a:RPE | STAR133PE | 0.1 mg | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Bovine CD3 | MCA6080 | 0.1 mg | F IC |
Product Specific References
References for CD5 antibody
-
Davis W.C. et al. (1987) The development and analysis of species specific and cross reactive monoclonal antibodies to leukocyte differentiation antigens and antigens of the major histocompatibility complex for use in the study of the immune system in cattle and other species.
Vet Immunol Immunopathol. 15 (4): 337-76.
