Mouse anti Bovine CD5, clone B29A
, recognizes bovine CD5. The 67 kDa glycoprotein CD5 is present on alpha beta and gamma delta T cells but only on a small number of B cells. In contrast, in cattle infected with bovine leukemia virus almost all B cells were CD5+ (Depelchin et al. 1989
). CD5 associates with CD2 and the CD3 zeta chain (Burgess et al. 1992
Similarly to mouse or human, in cattle CD5 associates with the B cell receptor. However, in cattle infected with bovine leukemia virus CD5 dissociates from the B cell receptor (Cantor et al. 2001
- Target Species
- Product Form
- Concentrated tissue culture supernatant - liquid
- Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
- Buffer Solution
- Serum free tissue culture medium containing proprietary protein free supplement
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Bovine lymphocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at +4oC. DO NOT FREEZE.
Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to membrane
scavenger receptor activity
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD5 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
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Secondary Antibodies Available
Useful Reagents Available
|Mouse anti Bovine CD3
Product Specific References
References for CD5 antibody
Davis W.C. et al. (1987) The development and analysis of species specific and cross reactive monoclonal antibodies to leukocyte differentiation antigens and antigens of the major histocompatibility complex for use in the study of the immune system in cattle and other species.
Vet Immunol Immunopathol. 15 (4): 337-76.