CD5 antibody | B29A

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Mouse anti Bovine CD5

Product Type
Monoclonal Antibody
Clone
B29A
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA6082 F 0.1 mg
Mouse anti Bovine CD5, clone B29A, recognizes bovine CD5. The 67 kDa glycoprotein CD5 is present on alpha beta and gamma delta T cells but only on a small number of B cells. In contrast, in cattle infected with bovine leukemia virus almost all B cells were CD5+ (Depelchin et al. 1989). CD5 associates with CD2 and the CD3 zeta chain (Burgess et al. 1992).
Similarly to mouse or human, in cattle CD5 associates with the B cell receptor. However, in cattle infected with bovine leukemia virus CD5 dissociates from the B cell receptor (Cantor et al. 2001).

Product Details

Target Species
Bovine
Product Form
Concentrated tissue culture supernatant - liquid
Preparation
Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
Buffer Solution
Serum free tissue culture medium containing proprietary protein free supplement
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Bovine lymphocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P19238 Related reagents
Entrez Gene
CD5 Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0005044 scavenger receptor activity
Regulatory
For research purposes only

Applications of CD5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG2a:RPE STAR133PE 0.1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Bovine CD3 MCA6080 0.1 mg F IC

Product Specific References

References for CD5 antibody

  1. Davis W.C. et al. (1987) The development and analysis of species specific and cross reactive monoclonal antibodies to leukocyte differentiation antigens and antigens of the major histocompatibility complex for use in the study of the immune system in cattle and other species.
    Vet Immunol Immunopathol. 15 (4): 337-76.