CD32 antibody | CCG39
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F ResetMouse anti Bovine CD32
- Product Type
- Monoclonal Antibody
- Clone
- CCG39
- Isotype
- IgG2a
- Specificity
- CD32
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| Mouse anti Bovine CD32 antibody, clone CCG39, recognizes the bovine homologue of human CD32, one of a group of Fc receptors belonging to the immunoglobulin superfamily and involved in phagocytosis of opsonized microbes. Bovine CD32 is a single pass type 1 membrane protein of approximately 32 kDa, expressed on the cell surface of most cells including B-lymphocytes, monocytes, neutrophils and afferent veiled lymph dendritic cells (Chattha et al. 2010). It has been shown that expression of bovine CD32 is higher on macrophages than on neutrophils. CD32 can function in an inhibtory capacity to antibody production and is the low affinity Fc receptor for IgG (FcRII), binding to the Fc region of immunoglobulin gamma Chattha et al. 2009. Mouse anti Bovine CD32, clone CCG39 is one of a number of anti bovine CD32 reagents available from Bio-Rad, clone CCG39 is of interest in that it is specific to bovine CD32 and does not recognizes ovine CD32. For detection of ovine CD32, clone CCG36, which cross reacts with ovine CD32, may be used. |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Bovine FcγRII-transfected COS7 cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS-1myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/50 |
| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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| Description | Mouse IgG2a Negative Control | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Bovine CD32:FITC | MCA5918F | F | 0.1 mg |
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| Description | Mouse anti Bovine CD32:FITC | ||||||
| Mouse anti Bovine CD32 | MCA5918GA | C F | 0.1 mg |
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| Description | Mouse anti Bovine CD32 | ||||||
| Mouse anti Bovine CD32:RPE | MCA5918PE | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Bovine CD32:RPE | ||||||
Further Reading
-
Kacskovics, I. (2004) Fc receptors in livestock species.
Vet Immunol Immunopathol. 102 (4): 351-62. -
Chattha, K.S. et al. (2009) Age related variation in expression of CD21 and CD32 on bovine lymphocytes: a cross-sectional study.
Vet Immunol Immunopathol. 130 (1-2): 70-8. -
Chattha, K.S. et al. (2010) Immunohistochemical investigation of cells expressing CD21, membrane IgM, CD32 and a follicular dendritic cell marker in the lymphoid tissues of neonatal calves.
Vet Immunol Immunopathol. 137 (3-4): 284-90.
- Synonyms
- FcRII
- UniProt
- Q28110
- Entrez Gene
- FCGR2
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0019864 IgG binding
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