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CD32 antibody | CCG39

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Anti Bovine CD32 Antibody, clone CCG39 thumbnail image 1 Anti Bovine CD32 Antibody, clone CCG39 thumbnail image 2 Anti Bovine CD32 Antibody, clone CCG39 thumbnail image 3
Anti Bovine CD32 Antibody, clone CCG39 gallery image 1
Figure A. Alexa Fluor 647 conjugated Human anti Bovine CD282 (HCA151A647) and FITC conjugated Mouse IgG2a isotype control (MCA929F). Figure B. Alexa Fluor 647 conjugated Human anti Bovine CD282 (HCA151A647) and FITC conjugated Mouse anti Bovine CD32 (MCA5919F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells in the presence of 10% bovine serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Bovine CD32 Antibody, clone CCG39 gallery image 2
Figure A. RPE conjugated Mouse anti Bovine CD205 (MCA2450PE) and FITC conjugated Mouse IgG2a isotype control (MCA929F). Figure B. RPE conjugated Mouse anti Bovine CD205 (MCA2450PE) and FITC conjugated Mouse anti Bovine CD32 (MCA5919F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Anti Bovine CD32 Antibody, clone CCG39 gallery image 3
Figure A. FITC conjugated Mouse anti Bovine CD32 (MCA5919F) and RPE conjugated Mouse IgG1 isotype control (MCA929PE). Figure B. FITC conjugated Mouse anti Bovine CD32 (MCA5919F) and RPE conjugated Mouse anti Bovine CD205 (MCA2450PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse anti Bovine CD32:FITC

Mouse anti Bovine CD32

Product Type
Monoclonal Antibody
Clone
CCG39
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA5919F
Datasheet
F
SDS
0.1 mg loader

MCA5919GA
Datasheet
C F
SDS
0.1 mg loader

Mouse anti Bovine CD32 antibody, clone CCG39, recognizes the bovine homologue of human CD32, one of a group of Fc receptors belonging to the immunoglobulin superfamily and involved in phagocytosis of opsonized microbes. Bovine CD32 is a single pass type 1 membrane protein of approximately 32 kDa, expressed on the cell surface of most cells including B-lymphocytes, monocytes, neutrophils and afferent veiled lymph dendritic cells (Chattha et al. 2010). It has been shown that expression of bovine CD32 is higher on macrophages than on neutrophils.

CD32 can function in an inhibtory capacity to antibody production and is the low affinity Fc receptor for IgG (FcRII), binding to the Fc region of immunoglobulin gamma Chattha et al. 2009.

Mouse anti Bovine CD32, clone CCG39 is one of a number of anti bovine CD32 reagents available from Bio-Rad, clone CCG39 is of interest in that it is specific to bovine CD32 and does not recognizes ovine CD32. For detection of ovine CD32, clone CCG36, which cross reacts with ovine CD32, may be used.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - Liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Bovine FcγRII-transfected COS7 cells.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS-1myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
Q28110
Entrez Gene
FCGR2
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0019864 IgG binding
Regulatory
For research purposes only

Applications of CD32 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/10 1/50
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F F 0.1 mg loader
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2a Negative Control:FITC MCA929F F 100 Tests loader
Mouse IgG2a Negative Control MCA929 F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Bovine CD32:FITC MCA5918F F 0.1 mg loader
Mouse anti Bovine CD32 MCA5918GA C F 0.1 mg loader
Mouse anti Bovine CD32:RPE MCA5918PE F 100 Tests loader
Mouse anti Bovine CD32:FITC MCA5918F F 0.1 mg loader
Mouse anti Bovine CD32 MCA5918GA C F 0.1 mg loader
Mouse anti Bovine CD32:RPE MCA5918PE F 100 Tests loader

Product Specific References

Further Reading

  1. Kacskovics, I. (2004) Fc receptors in livestock species.
    Vet Immunol Immunopathol. 102 (4): 351-62.
  2. Chattha, K.S. et al. (2009) Age related variation in expression of CD21 and CD32 on bovine lymphocytes: a cross-sectional study.
    Vet Immunol Immunopathol. 130 (1-2): 70-8.
  3. Chattha, K.S. et al. (2010) Immunohistochemical investigation of cells expressing CD21, membrane IgM, CD32 and a follicular dendritic cell marker in the lymphoid tissues of neonatal calves.
    Vet Immunol Immunopathol. 137 (3-4): 284-90.

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