CD282 antibody | AbD12538

Human anti Bovine CD282

Product Type
Monoclonal Antibody
Clone
AbD12538
Isotype
HuCAL Fab bivalent
Specificity
CD282

Product Code Applications Pack Size List Price Your Price Qty
HCA152
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Human anti Bovine CD282 antibody, clone AbD12538 recognizes bovine TLR2, otherwise known as CD282. TLR2 is a single-pass type 1 membrane protein belong to the Toll-like receptor (TLR) family and is expressed primarily by peripheral blood monocytes.

TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents. They also initiate cell signaling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity.

TLR2 is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with Ly96 and TLR1.

Target Species
Bovine
Product Form
A bivalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Fc-fusion protein containing the sequence 21-588 from bovine TLR2
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Histidine Tag:Alexa Fluor® 488 MCA1396A488 F 100 Tests/1ml loader
List Price Your Price
loader
Description Mouse anti Histidine Tag:Alexa Fluor® 488
Mouse anti Histidine Tag:Alexa Fluor® 647 MCA1396A647 F 1 ml loader
List Price Your Price
loader
Description Mouse anti Histidine Tag:Alexa Fluor® 647
Mouse anti Histidine Tag:FITC MCA1396F F IF 0.1 mg loader
List Price Your Price
loader
Description Mouse anti Histidine Tag:FITC
Mouse anti c-Myc:Alexa Fluor® 488 MCA2200A488 F* 100 Tests/1ml loader
List Price Your Price
loader
Description Mouse anti c-Myc:Alexa Fluor® 488
Mouse anti c-Myc:Alexa Fluor® 647 MCA2200A647 F* 100 Tests/1ml loader
List Price Your Price
loader
Description Mouse anti c-Myc:Alexa Fluor® 647
Mouse anti c-Myc:Biotin MCA2200B C E P WB* 0.1 mg loader
List Price Your Price
loader
Description Mouse anti c-Myc:Biotin
Mouse anti c-Myc:FITC MCA2200F F* 0.1 mg loader
List Price Your Price
loader
Description Mouse anti c-Myc:FITC
Mouse anti Penta Histidine Tag:HRP MCA5995P E WB 125 µl loader
List Price Your Price
loader
Description Mouse anti Penta Histidine Tag:HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
HuCAL Fab-dHLX-MH Negative Control HCA052 E 0.1 mg loader
List Price Your Price
loader
Description HuCAL Fab-dHLX-MH Negative Control

References for CD282 antibody

  1. Kwong, L.S. et al. (2011) Characterisation of antibodies to bovine Toll-like receptor (TLR)-2 and cross-reactivity with ovine TLR2.
    Vet Immunol Immunopathol. 139: 313-8.

Synonyms
TLR2
RRID
AB_11152778
UniProt
Q95LA9
Entrez Gene
TLR2
GO Terms
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0006954 inflammatory response
GO:0045087 innate immune response
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
View more products with CD282 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Bovine Products