CD282 antibody | AbD12538

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Human anti Bovine CD282:Alexa Fluor® 647

Human anti Bovine CD282:FITC

Human anti Bovine CD282

Product Type
Monoclonal Antibody
Clone
AbD12538
Isotype
HuCAL Fab bivalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA152A647 F 100 Tests/1ml
HCA152F F 0.1 mg
HCA152 F 0.1 mg
Human anti Bovine CD282 antibody, clone AbD12538 recognizes bovine TLR2, otherwise known as CD282. TLR2 is a single-pass type 1 membrane protein belong to the Toll-like receptor (TLR) family and is expressed primarily by peripheral blood monocytes.

TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents. They also initiate cell signaling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity.

TLR2 is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with Ly96 and TLR1.

Product Details

Target Species
Bovine
Product Form
A bivalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is conjugated to Alexa Fluor 647 - liquid.
Product Form
A bivalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is conjugated to fluorescein isothiocyanate (FITC) - liquid.
Product Form
A bivalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
Preparation
Metal chelate affinity chromatography
Preparation
Metal chelate affinity chromatography
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Fc-fusion protein containing the sequence 21-588 from bovine TLR2
Approx. Protein Concentrations
Ig concentration 0.05 mg/ml
Approx. Protein Concentrations
Ig concentration 0.1 mg/ml
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Shelf Life
12 months from date of despatch
Shelf Life
12 months from date of despatch
Shelf Life
12 months from date of despatch

More Information

UniProt
Q95LA9 Related reagents
Entrez Gene
TLR2 Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0006954 inflammatory response
GO:0045087 innate immune response
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents.

His-tag is a registered trademark of EMD Biosciences.

This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of CD282 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry Neat
Flow Cytometry 1/10 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Histidine Tag:Alexa Fluor® 488 MCA1396A488 100 Tests/1ml F
Mouse anti Histidine Tag:Alexa Fluor® 647 MCA1396A647 1 ml F
Mouse anti Histidine Tag:FITC MCA1396F 0.1 mg F IF
Mouse anti c-Myc:Alexa Fluor® 488 MCA2200A488 100 Tests/1ml F*
Mouse anti c-Myc:Alexa Fluor® 647 MCA2200A647 100 Tests/1ml F*
Mouse anti c-Myc:Biotin MCA2200B 0.1 mg C E P WB*
Mouse anti c-Myc:FITC MCA2200F 0.1 mg F*
Mouse anti c-Myc:RPE MCA2200PE 100 Tests WB
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
HuCAL Fab-dHLX-MH Negative Control:Alexa Fluor® 647 HCA052A647 1 ml E F
HuCAL Fab-dHLX-MH Negative Control:FITC HCA052F 0.1 mg C E F P WB
HuCAL Fab-dHLX-MH Negative Control HCA052 0.1 mg C E F P WB

Product Specific References

References for CD282 antibody

  1. Kwong, L.S. et al. (2011) Characterisation of antibodies to bovine Toll-like receptor (TLR)-2 and cross-reactivity with ovine TLR2.
    Vet Immunol Immunopathol. 139: 313-8.