CD282 antibody | AbD12538
Human anti Bovine CD282 antibody, clone AbD12538 recognizes bovine TLR2, otherwise known as CD282. TLR2 is a single-pass type 1 membrane protein belong to the Toll-like receptor (TLR) family and is expressed primarily by peripheral blood monocytes.
TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents. They also initiate cell signaling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity.
TLR2 is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with Ly96 and TLR1.
TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents. They also initiate cell signaling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity.
TLR2 is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with Ly96 and TLR1.
Product Details
- Target Species
- Bovine
- Product Form
- A bivalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is conjugated to Alexa Fluor 647 - liquid.
- Product Form
- A bivalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
- Preparation
- Metal chelate affinity chromatography
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Fc-fusion protein containing the sequence 21-588 from bovine TLR2
- Approx. Protein Concentrations
- Ig concentration 0.05 mg/ml
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q95LA9
- Entrez Gene
- TLR2
- GO Terms
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0006954 inflammatory response
- GO:0045087 innate immune response
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com - Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences. - Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
- Regulatory
- For research purposes only
Applications of CD282 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/10 | 1/50 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Histidine Tag:Alexa Fluor® 488 | MCA1396A488 | F | 100 Tests/1ml |
|
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| Mouse anti Histidine Tag:Alexa Fluor® 647 | MCA1396A647 | F | 1 ml |
|
|
| Mouse anti Histidine Tag:FITC | MCA1396F | F IF | 0.1 mg |
|
|
| Mouse anti c-Myc:Alexa Fluor® 488 | MCA2200A488 | F* | 100 Tests/1ml |
|
|
| Mouse anti c-Myc:Alexa Fluor® 647 | MCA2200A647 | F* | 100 Tests/1ml |
|
|
| Mouse anti c-Myc:Biotin | MCA2200B | C E P WB* | 0.1 mg |
|
|
| Mouse anti c-Myc:FITC | MCA2200F | F* | 0.1 mg |
|
|
| Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| HuCAL Fab-dHLX-MH Negative Control:Alexa Fluor® 647 | HCA052A647 | F | 1 ml |
|
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| HuCAL Fab-dHLX-MH Negative Control | HCA052 | E | 0.1 mg |
|
Product Specific References
References for CD282 antibody
-
Kwong, L.S. et al. (2011) Characterisation of antibodies to bovine Toll-like receptor (TLR)-2 and cross-reactivity with ovine TLR2.
Vet Immunol Immunopathol. 139: 313-8.
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