Mouse anti Bovine CD1w2 antibody, clone CC14 recognizes the CD1w2 cell surface antigen in bovine, sheep and goat.
Mouse anti Bovine CD1w2 antibody, clone CC14 immunoprecipitates two protein bands of ~12 kDa and ~46 kDa. In immunohistology the reagent stains cortical thymocytes and a minority of medullary thymocytes, with a pattern similar to antibodies of the CD1b cluster in humans.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD1w2 antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Product Specific References
References for CD1w2 antibody
Machugh, N.D. et al. (1988) Characterization of a bovine thymic differentiation antigen analogous to CD1 in the human.
Scand J Immunol. 27 (5): 541-7.
(1991) Comparison of reactivity of monoclonal antibodies on bovine, ovine and caprine tissues and on cells from other animal species.
Vet Immunol Immunopathol. 27 (1-3): 32-4.
Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.
Vet Immunol Immunopathol. 146: 1-7.
Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.
J Virol. 79: 7738-44.
Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells.
J Gen Virol. 84: 1771-80.
Jörundsson, E. et al. (2000) Distribution of MHC-II and CD1 molecules in the skin of lambs and changes during experimentally-induced contact hypersensitivity.
Vet Immunol Immunopathol. 74: 87-101.
Pirson, C. et al. (2012) Differential effects of Mycobacterium bovis - derived polar and apolar lipid fractions on bovine innate immune cells.
Vet Res. 43: 54.
Stephens, S.A. and Howard, C.J. (2002) Infection and transformation of dendritic cells from bovine afferent lymph by Theileria annulata.
Parasitology. 124: 485-93.
Valheim, M. et al. (2002) Lesions in subclinical paratuberculosis of goats are associated with persistent gut-associated lymphoid tissue.
J Comp Pathol. 127: 194-202.
Corripio-Miyar, Y. et al. (2015) Phenotypic and functional analysis of monocyte populations in cattle peripheral blood identifies a subset with high endocytic and allogeneic T-cell stimulatory capacity.
Vet Res. 46 (1): 112.
Corripio-miyar, Y. et al. (2017) 1,25-Dihydroxyvitamin D3 modulates the phenotype and function of Monocyte derived dendritic cells in cattle.
BMC Vet Res. 13 (1): 390.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra