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CD1w2 antibody | CC14

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Anti Bovine CD1w2 Antibody, clone CC14 thumbnail image 1 Anti Bovine CD1w2 Antibody, clone CC14 thumbnail image 2
Anti Bovine CD1w2 Antibody, clone CC14 gallery image 1
Published customer image:
Mouse anti Bovine CD1w2 antibody, clone CC14 (MCA831G) used for the evaluation of CD1w2 expression on bovine monocyte derived dendritic cells by flow cytometry.
Image caption:
Surface expression of MHCII (A), CD86 (B), CD1b (C) and CD40 (D) on cultured cells and fresh monocytes after exposure to crude polar and apolar lipid fractions. A single point represents the median fluorescence intensity of the specific stain after subtraction of an isotype control (ΔMFI) for each of 7 animals tested; ** p < 0.01; *** p < 0.001.

From: Pirson C, Jones GJ, Steinbach S, Besra GS, Vordermeier HM. Differential effects of Mycobacterium bovis--derived polar and apolar lipid fractions on bovine innate immune cells.
Vet Res. 2012 Jun 27;43:54.
Anti Bovine CD1w2 Antibody, clone CC14 gallery image 2
Published customer image:
Mouse anti Bovine CD1w2 antibody, clone CC14 (MCA831G) used for the evaluation of CD1w2 expression on bovine myeloid cell subtypes by flow cytometry.
Image caption:
Phenotypic profiles of bovine myeloid cell sub-populations. Live gated PBMC were assessed for expression of CD16 and CD14 and a panel of molecules associated with antigen presentation, co-stimulation or specific cell lineages (see Table 1) by 3 colour flow cytometry. PBMC were stained with primary mAb to the specific molecules indicated and then with an isotype-specific PE conjugated secondary, followed by CD16 and CD14 conjugated to FITC or Alexa Fluor 647 respectively. Live, single PBMC were gated based on the expression of CD16 and CD14 as detailed in Figures 1 and 2C. Histograms show the levels of expression of selected markers in the cell populations studied, CD14 − CD16 ++ (red), CD14 + CD16 + (blue) and CD14 + CD16 low/- (orange) compared to PBMC (black). Data shown are for one representative animal of four animals.

From: Corripio-Miyar Y, Hope J, McInnes CJ, Wattegedera SR, Jensen K, Pang Y, Entrican G, Glass EJ. Phenotypic and functional analysis of monocyte populations in cattle peripheral blood identifies a subset with high endocytic and allogeneic T-cell stimulatory capacity.
Vet Res. 2015 Sep 25;46(1):112.

Mouse anti Bovine CD1w2

Product Type
Monoclonal Antibody
Clone
CC14
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA831G
Datasheet
C F IP
SDS
0.25 mg loader

Mouse anti Bovine CD1w2 antibody, clone CC14 recognizes the CD1w2 cell surface antigen in bovine, sheep and goat.

Mouse anti Bovine CD1w2 antibody, clone CC14 immunoprecipitates two protein bands of ~12 kDa and ~46 kDa. In immunohistology the reagent stains cortical thymocytes and a minority of medullary thymocytes, with a pattern similar to antibodies of the CD1b cluster in humans.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of CD1w2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Application Based External Images

Flow Cytometry

Product Specific References

References for CD1w2 antibody

  1. Machugh, N.D. et al. (1988) Characterization of a bovine thymic differentiation antigen analogous to CD1 in the human.
    Scand J Immunol. 27 (5): 541-7.
  2. (1991) Comparison of reactivity of monoclonal antibodies on bovine, ovine and caprine tissues and on cells from other animal species.
    Vet Immunol Immunopathol. 27 (1-3): 32-4.
  3. Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.
    Vet Immunol Immunopathol. 146: 1-7.
  4. Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.
    J Virol. 79: 7738-44.
  5. Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells.
    J Gen Virol. 84: 1771-80.
  6. Jörundsson, E. et al. (2000) Distribution of MHC-II and CD1 molecules in the skin of lambs and changes during experimentally-induced contact hypersensitivity.
    Vet Immunol Immunopathol. 74: 87-101.
  7. Pirson, C. et al. (2012) Differential effects of Mycobacterium bovis - derived polar and apolar lipid fractions on bovine innate immune cells.
    Vet Res. 43: 54.
  8. Stephens, S.A. and Howard, C.J. (2002) Infection and transformation of dendritic cells from bovine afferent lymph by Theileria annulata.
    Parasitology. 124: 485-93.
  9. Valheim, M. et al. (2002) Lesions in subclinical paratuberculosis of goats are associated with persistent gut-associated lymphoid tissue.
    J Comp Pathol. 127: 194-202.
  10. Corripio-Miyar, Y. et al. (2015) Phenotypic and functional analysis of monocyte populations in cattle peripheral blood identifies a subset with high endocytic and allogeneic T-cell stimulatory capacity.
    Vet Res. 46 (1): 112.
  11. Corripio-miyar, Y. et al. (2017) 1,25-Dihydroxyvitamin D3 modulates the phenotype and function of Monocyte derived dendritic cells in cattle.
    BMC Vet Res. 13 (1): 390.

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