CD1w2 antibody | CC14
Mouse anti Bovine CD1w2 antibody, clone CC14 used for the evaluation of CD1w2 expression on bovine monocyte derived dendritic cells by flow cytometry.
Image caption:
Surface expression of MHCII (A), CD86 (B), CD1b (C) and CD40 (D) on cultured cells and fresh monocytes after exposure to crude polar and apolar lipid fractions. A single point represents the median fluorescence intensity of the specific stain after subtraction of an isotype control (ΔMFI) for each of 7 animals tested; ** p < 0.01; *** p < 0.001.
From: Pirson C, Jones GJ, Steinbach S, Besra GS, Vordermeier HM. Differential effects of Mycobacterium bovis--derived polar and apolar lipid fractions on bovine innate immune cells.
Vet Res. 2012 Jun 27;43:54.
Mouse anti Bovine CD1w2 antibody, clone CC14 used for the evaluation of CD1w2 expression on bovine myeloid cell subtypes by flow cytometry.
Image caption:
Phenotypic profiles of bovine myeloid cell sub-populations. Live gated PBMC were assessed for expression of CD16 and CD14 and a panel of molecules associated with antigen presentation, co-stimulation or specific cell lineages (see Table 1) by 3 colour flow cytometry. PBMC were stained with primary mAb to the specific molecules indicated and then with an isotype-specific PE conjugated secondary, followed by CD16 and CD14 conjugated to FITC or Alexa Fluor 647 respectively. Live, single PBMC were gated based on the expression of CD16 and CD14 as detailed in Figures 1 and 2C. Histograms show the levels of expression of selected markers in the cell populations studied, CD14 − CD16 ++ (red), CD14 + CD16 + (blue) and CD14 + CD16 low/- (orange) compared to PBMC (black). Data shown are for one representative animal of four animals.
From: Corripio-Miyar Y, Hope J, McInnes CJ, Wattegedera SR, Jensen K, Pang Y, Entrican G, Glass EJ. Phenotypic and functional analysis of monocyte populations in cattle peripheral blood identifies a subset with high endocytic and allogeneic T-cell stimulatory capacity.
Vet Res. 2015 Sep 25;46(1):112.
Mouse anti Bovine CD1w2 antibody, clone CC14 recognizes the CD1w2 cell surface antigen in bovine, sheep and goat.
Mouse anti Bovine CD1w2 antibody, clone CC14 immunoprecipitates two protein bands of ~12 kDa and ~46 kDa. In immunohistology the reagent stains cortical thymocytes and a minority of medullary thymocytes, with a pattern similar to antibodies of the CD1b cluster in humans.
Mouse anti Bovine CD1w2 antibody, clone CC14 immunoprecipitates two protein bands of ~12 kDa and ~46 kDa. In immunohistology the reagent stains cortical thymocytes and a minority of medullary thymocytes, with a pattern similar to antibodies of the CD1b cluster in humans.
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of CD1w2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/50 | 1/100 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F |
Product Specific References
References for CD1w2 antibody
-
Machugh, N.D. et al. (1988) Characterization of a bovine thymic differentiation antigen analogous to CD1 in the human.
Scand J Immunol. 27 (5): 541-7. -
(1991) Comparison of reactivity of monoclonal antibodies on bovine, ovine and caprine tissues and on cells from other animal species.
Vet Immunol Immunopathol. 27 (1-3): 32-4. -
Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.
Vet Immunol Immunopathol. 146: 1-7. -
Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.
J Virol. 79: 7738-44. -
Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells.
J Gen Virol. 84: 1771-80. -
Jörundsson, E. et al. (2000) Distribution of MHC-II and CD1 molecules in the skin of lambs and changes during experimentally-induced contact hypersensitivity.
Vet Immunol Immunopathol. 74: 87-101. -
Pirson, C. et al. (2012) Differential effects of Mycobacterium bovis - derived polar and apolar lipid fractions on bovine innate immune cells.
Vet Res. 43: 54. -
Stephens, S.A. and Howard, C.J. (2002) Infection and transformation of dendritic cells from bovine afferent lymph by Theileria annulata.
Parasitology. 124: 485-93. -
Valheim, M. et al. (2002) Lesions in subclinical paratuberculosis of goats are associated with persistent gut-associated lymphoid tissue.
J Comp Pathol. 127: 194-202. -
Corripio-Miyar, Y. et al. (2015) Phenotypic and functional analysis of monocyte populations in cattle peripheral blood identifies a subset with high endocytic and allogeneic T-cell stimulatory capacity.
Vet Res. 46 (1): 112. -
Corripio-Miyar, Y. et al. (2017) 1,25-Dihydroxyvitamin D3 modulates the phenotype and function of Monocyte derived dendritic cells in cattle
BMC Veterinary Research. 13 (1) [Epub ahead of print].
