CD172a antibody | CC149
Mouse anti Bovine CD172a:RPE-Cy5
- Product Type
- Monoclonal Antibody
- Clone
- CC149
- Isotype
- IgG2b
- Specificity
- CD172a
Mouse anti Bovine CD172a antibody, clone CC149 recognizes bovine CD172a, also known as MyD-1 antigen and SIRPA. CD172a is a ~55 kDa single pass type 1 membrane protein belonging to the family of signal regulatory proteins (SIRP). CD172a has been identified as the receptor for CD47. Bovine CD172a is strongly expressed by splenic macrophages, monocytes and a subset of afferent lymph veiled cells (ALVC) and by dendritic cells in the skin. |
- Target Species
- Bovine
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) -Cy5 - lyophilized
- Reconstitution
- Reconstitute with 1.0ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose - Immunogen
- Bovine afferent veiled dendritic cells
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE-Cy5 488nm laser 496 667 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- Cy and CyDye are registered trademarks of GE Healthcare
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG2b Negative Control:RPE-Cy5 | MCA691C | F | 100 Tests | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse IgG2b Negative Control:RPE-Cy5 |
References for CD172a antibody
-
Smith, R. et al. (2003) A novel MyD-1 (SIRP-1alpha) signaling pathway that inhibits LPS-induced TNFalpha production by monocytes.
Blood.102: 2532-40. -
Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.
J Virol. 79: 7738-44. -
Price, S.J. & Hope, J.C.. (2009) Enhanced secretion of interferon-gamma by bovine gammadelta T cells induced by coculture with Mycobacterium bovis-infected dendritic cells: evidence for reciprocal activating signals.
Immunology. 126:201-8 -
Waters, W.R. (2009) Signal regulatory protein alpha (SIRPalpha) cells in the adaptive response to ESAT-6/CFP-10 protein of tuberculous mycobacteria.
PLoS One. 4: e6414. -
Jensen, K. et al. (2014) Comparison of small interfering RNA (siRNA) delivery into bovine monocyte-derived macrophages by transfection and electroporation.
Vet Immunol Immunopathol. 158 : 224-32. -
Hussen J et al. (2014) The chemokine CCL5 induces selective migration of bovine classical monocytes and drives their differentiation into LPS-hyporesponsive macrophages in vitro.
Dev Comp Immunol. 47 (2): 169-77. -
Eger, M. et al. (2015) Impacts of parturition and body condition score on glucose uptake capacity of bovine monocyte subsets.
Vet Immunol Immunopathol. 166 (1-2): 33-42. -
Vachiery N et al. (2015) An in vitro model to assess the immunosuppressive effect of tick saliva on the mobilization of inflammatory monocyte-derived cells.
Vet Res. 46 (1): 117.
View The Latest Product References
-
Tahoun, A. et al. (2015) Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella.
Vet Res. 46: 9. -
Pridans, C. et al. (2016) A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep.
J Immunol. 197 (6): 2297-305. -
Herry, V. et al. (2017) Local immunization impacts the response of dairy cows to Escherichia coli mastitis.
Sci Rep. 7 (1): 3441. -
Liu, J. et al. (2020) Theileria annulata. transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells.
Ticks Tick Borne Dis. 11 (3): 101365. -
Okino, C.H. et al. (2020) A polymorphic CD4 epitope related to increased susceptibility to Babesia bovis in Canchim calves.
Vet Immunol Immunopathol. 230: 110132. -
Barut, G.T. et al. (2020) Transcriptomic profiling of bovine blood dendritic cells and monocytes following TLR stimulation.
Eur J Immunol. 50 (11): 1691-711. -
Kolar, Q.K. et al. (2020) Anatomical distribution of respiratory tract leukocyte cell subsets in neonatal calves.
Vet Immunol Immunopathol. 227: 110090. -
Park, D.S. et al. (2021) Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress.
Cell Stress Chaperones. 26 (4): 705-20. -
Ibeagha-Awemu, E.M. et al. (2021) Regionally Distinct Immune and Metabolic Transcriptional Responses in the Bovine Small Intestine and Draining Lymph Nodes During a Subclinical Mycobacterium avium subsp. paratuberculosis Infection.
Front Immunol. 12: 760931. -
Marzo, S. et al. (2022) Characterisation of dendritic cell frequency and phenotype in bovine afferent lymph reveals kinetic changes in costimulatory molecule expression.
Vet Immunol Immunopathol. 243: 110363. -
Tahoun, A. et al. (2020) Inflammasome Activation in Bovine Peripheral Blood-Derived Macrophages Is Associated with Actin Rearrangement.
Animals (Basel). 10 (4): 655. -
Casaro, S. et al. (2022) Flow cytometry panels for immunophenotyping dairy cattle peripheral blood leukocytes
Vet Immunol Immunopathol. 248: 110417.
Further Reading
-
Howard, C.J. et al. (1999) Dendritic cells in cattle: phenotype and function.
Vet Immunol Immunopathol. 72 (1-2): 119-24.
- Synonyms
- SIRP ALPHA
- UniProt
- O46631
- Entrez Gene
- SIRPA
- GO Terms
- GO:0016021 integral to membrane
- GO:0017124 SH3 domain binding
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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