Tropomodulin 1 antibody | TRM-17 (Mab17)
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Mouse anti Tropomodulin 1 (N-Terminal) antibody, clone TRM-17 recognizes chicken tropomodulin 1, otherwise known as erythrocyte tropomodulin or E-Tmod, an evolutionary very highly conserved cytoskeletal protein .
Tropomodulin 1 is a sarcomeric protein highly expressed by erythrocytes, heart and skeletal muscle, which binds to and caps pointed ends of actin filaments to prevent thin filament elongation. The binding of the N-terminal region of Tmod1 with the actin-binding protein tropomyosin blocks the elongation and depolymerization of actin filaments, contributing to the geometry of the membrane skeleton.
Mouse anti Tropomodulin 1 (N-Terminal) antibody, clone TRM-17 recognizes an epitope between amino acid residues 6-57 within the N-terminal (NT) region of tropomodulin 1 and inhibits the binding of Tmod1 to tropomyosin.
Tropomodulin 1 is a sarcomeric protein highly expressed by erythrocytes, heart and skeletal muscle, which binds to and caps pointed ends of actin filaments to prevent thin filament elongation. The binding of the N-terminal region of Tmod1 with the actin-binding protein tropomyosin blocks the elongation and depolymerization of actin filaments, contributing to the geometry of the membrane skeleton.
Mouse anti Tropomodulin 1 (N-Terminal) antibody, clone TRM-17 recognizes an epitope between amino acid residues 6-57 within the N-terminal (NT) region of tropomodulin 1 and inhibits the binding of Tmod1 to tropomyosin.
Product Details
- Target Species
- Chicken
- Species Cross-Reactivity
-
Target Species Cross Reactivity Birds Mammals Fish Expected from Sequence Insect Expected from Sequence Reptile Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.05% Sodium Azide (NaN3)
- Immunogen
- Recombinant chicken tropomodulin 1
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of Tropomodulin 1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Functional Assays 1 | |||
| Immunofluorescence | |||
| Western Blotting |
- 1 Removal of sodium azide is recommended prior to use in functional assays. Bio-Rad recommend EQU003 for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- MCA4760 detects a band of approximately 40kDa in chick heart lysates.
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Secondary Antibodies Available
Product Specific References
References for Tropomodulin 1 antibody
-
Mudry, R.E. et al. (2003) The interaction of tropomodulin with tropomyosin stabilizes thin filaments in cardiac myocytes.
J Cell Biol. 162 (6): 1057-68. -
Almenar-Queralt, A. et al. (1999) Identification of a novel tropomodulin isoform, skeletal tropomodulin, that caps actin filament pointed ends in fast skeletal muscle
J Biol Chem 274: 28466-75
