Find out more about A.I.den - our SearchBot

Tropomodulin 1 antibody | TRM-17 (Mab17)

100% Secure
No associated image currently available
No associated image currently available

Mouse anti Tropomodulin 1 (N-Terminal)

Product Type
Monoclonal Antibody
Clone
TRM-17 (Mab17)
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA4760
Datasheet
E FN* IF WB
SDS
0.25 mg loader

Mouse anti Tropomodulin 1 (N-Terminal) antibody, clone TRM-17 recognizes chicken tropomodulin 1, otherwise known as erythrocyte tropomodulin or E-Tmod, an evolutionary very highly conserved cytoskeletal protein .

Tropomodulin 1 is a sarcomeric protein highly expressed by erythrocytes, heart and skeletal muscle, which binds to and caps pointed ends of actin filaments to prevent thin filament elongation. The binding of the N-terminal region of Tmod1 with the actin-binding protein tropomyosin blocks the elongation and depolymerization of actin filaments, contributing to the geometry of the membrane skeleton.

Mouse anti Tropomodulin 1 (N-Terminal) antibody, clone TRM-17 recognizes an epitope between amino acid residues 6-57 within the N-terminal (NT) region of tropomodulin 1 and inhibits the binding of Tmod1 to tropomyosin.

Product Details

Target Species
Chicken
Species Cross-Reactivity
Target SpeciesCross Reactivity
Birds
Mammals
Fish Expected from Sequence
Insect Expected from Sequence
Reptile Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05% Sodium Azide (NaN3)
Immunogen
Recombinant chicken tropomodulin 1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of Tropomodulin 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays 1
Immunofluorescence
Western Blotting
  1. 1 Removal of sodium azide is recommended prior to use in functional assays. Bio-Rad recommend EQU003 for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA4760 detects a band of approximately 40kDa in chick heart lysates.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for Tropomodulin 1 antibody

  1. Mudry, R.E. et al. (2003) The interaction of tropomodulin with tropomyosin stabilizes thin filaments in cardiac myocytes.
    J Cell Biol. 162 (6): 1057-68.
  2. Almenar-Queralt, A. et al. (1999) Identification of a novel tropomodulin isoform, skeletal tropomodulin, that caps actin filament pointed ends in fast skeletal muscle
    J Biol Chem 274: 28466-75

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer?

Watch the Tool Tutorial Video ▸

  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra
Request a different product with the Tropomodulin 1 specificity