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TCR Alpha/Beta antibody | TCR-2

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Anti Chicken TCR Alpha/Beta (Vb1) Antibody, clone TCR-2 gallery image 1
Published customer image:
Mouse anti Chicken TCRα/β antibody, clone L22 used for immunofluorescence studies.
Image caption:
F distribution of lymphoid aggregates in the MacRed chicken gut. (A-I) External views of different regions of a 1-year-old MacRed chicken showing several scattered lymphoid aggregates in the jejunum (A-C), numerous scattered lymphoid aggregates in the ileum (D-F) and a high concentration of lymphoid aggregates in the ileum Peyer's Patch. Scale bars in A-I: 500 μm. (J-L) Immunofluorescence staining of Peyer's patches showing organisation of CSF1R-mApple-expressing cells (red) in relation to: (J) Bu-1+ B-cells (green), (K) TCR αβ (Vβ1)+ T-cells (green) and (L) CVI-ChNL-74.2+ macrophages (green). Scale bars in J-L: 100 μm.

From: Balic A, Garcia-Morales C, Vervelde L, Gilhooley H, Sherman A, Garceau V, Gutowska MW, Burt DW, Kaiser P, Hume DA, Sang HM. Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage. Development. 2014 Aug;141(16):3255-65.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Chicken TCR Alpha/Beta (Vb1)

Product Type
Monoclonal Antibody
Clone
TCR-2
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA5771
Datasheet
C F IF IP
SDS
0.25 mg loader

Mouse anti Chicken TCRα/β antibody, clone TCR2 recognizes chicken TCRα/β expressed on peripheral blood T cells. Two distinct subpopulations of α/β T cells appear in the chicken thymus subsequent to the appearance of γ/δ T cells. These subpopulations, originally denoted as TCR2 and TCR3 arise sequentially in the thymus during ontogeny and are now known to represent two distinct Vβ families, Vβ1 and Vβ2, respectively.

Mouse anti Chicken TCRα/β antibody, clone TCR2 is specific for the Vβ1.

Product Details

Target Species
Chicken
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Borate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of TCR Alpha/Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for TCR Alpha/Beta antibody

  1. Balic, A. et al. (2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
    Development. 141: 3255-65.
  2. Röhe I. et al. (2017) Effect of feeding soybean meal and differently processed peas on the gut mucosal immune system of broilers
    Poultry Science. Feb 23 [Epub ahead of print]

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