Spectrin antibody | AA6 (7A3)
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Mouse anti Chicken spectrin, clone AA6 recognizes spectrin alpha chain, non-erythrocytic 1, also known as Alpha-II spectrin and Fodrin alpha chain, encoded by the SPTAN1 gene. Mouse anti chicken spectrin recognizes both chicken spectrin and the alpha chain of non-erythroid spectrin in mammalian cells.
Mouse anti Chicken spectrin, clone AA6 reacts with the 240 kDa full length spectrin alpha, the ~150 kDa, 145 kDa characteristic breakdown products of calpain and caspase-3 (Nath et al. 1996) and the ~120 kDa product, a result of caspase-3 activation (Nath et al. 1998).
Mouse anti Chicken spectrin, clone AA6 reacts with the 240 kDa full length spectrin alpha, the ~150 kDa, 145 kDa characteristic breakdown products of calpain and caspase-3 (Nath et al. 1996) and the ~120 kDa product, a result of caspase-3 activation (Nath et al. 1998).
Product Details
- Target Species
- Chicken
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rabbit Rat Guinea Pig Human Mouse Pig Mammals Expected from Sequence Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Chicken blood cell membrane purified by hypotonic lysis and mechanical enucleation.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P07751 Related reagents
- Entrez Gene
- SPTAN1 Related reagents
- GO Terms
- GO:0005509 calcium ion binding
- GO:0003779 actin binding
- GO:0005516 calmodulin binding
- GO:0005856 cytoskeleton
- GO:0005938 cell cortex
- GO:0051693 actin filament capping
- Regulatory
- For research purposes only
Applications of Spectrin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Chromatin Immunoprecipitation | |||
| Immunofluorescence | 1/200 | 1/400 | |
| Immunohistology - Paraffin | |||
| Immunoprecipitation | |||
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Clone AA6 recognizes the 150kDa and 120kDa spectrin cleavage products in staurosporine-treated HeLa lysates.
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Secondary Antibodies Available
Product Specific References
References for Spectrin antibody
-
Ylikoski, J. et al. (1992) Distribution of F-actin and fodrin in the hair cells of the guinea pig cochlea as revealed by confocal fluorescence microscopy.
Hear Res. 60 (1): 80-8. -
Ylikoski, J. et al. (1990) Nonerythroid spectrin (fodrin) is a prominent component of the cochlear hair cells.
Hear Res. 43 (2-3): 199-203.
