Spectrin antibody | AA6 (7A3)

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Mouse anti Chicken Spectrin (Non-Erythroid)

Product Type: Monoclonal Antibody
Clone: AA6 (7A3)
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
8365-1256	Ch IF IP WB			50 µg

Mouse anti Chicken spectrin, clone AA6 recognizes spectrin alpha chain, non-erythrocytic 1, also known as Alpha-II spectrin and Fodrin alpha chain, encoded by the SPTAN1 gene. Mouse anti chicken spectrin recognizes both chicken spectrin and the alpha chain of non-erythroid spectrin in mammalian cells.

Mouse anti Chicken spectrin, clone AA6 reacts with the 240 kDa full length spectrin alpha, the ~150 kDa, 145 kDa characteristic breakdown products of calpain and caspase-3 (Nath et al. 1996) and the ~120 kDa product, a result of caspase-3 activation (Nath et al. 1998).

Product Details

Target Species

Chicken

Species Cross-Reactivity

Target Species	Cross Reactivity
Rabbit
Rat
Guinea Pig
Human
Mouse
Pig
Mammals	Expected from Sequence
Human

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution

Phosphate buffered saline.

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin

Immunogen

Chicken blood cell membrane purified by hypotonic lysis and mechanical enucleation.

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.

More Information

UniProt: P07751 Related reagents
Entrez Gene: SPTAN1 Related reagents
GO Terms: GO:0005509 calcium ion binding; GO:0003779 actin binding; GO:0005516 calmodulin binding; GO:0005856 cytoskeleton; GO:0005938 cell cortex; GO:0051693 actin filament capping
Regulatory: For research purposes only

Applications of Spectrin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Chromatin Immunoprecipitation
Immunofluorescence	1/200	1/400
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting	1/500	1/2000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: Clone AA6 recognizes the 150kDa and 120kDa spectrin cleavage products in staurosporine-treated HeLa lysates.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for Spectrin antibody

Ylikoski, J. et al. (1992) Distribution of F-actin and fodrin in the hair cells of the guinea pig cochlea as revealed by confocal fluorescence microscopy.
Hear Res. 60 (1): 80-8.
Ylikoski, J. et al. (1990) Nonerythroid spectrin (fodrin) is a prominent component of the cochlear hair cells.
Hear Res. 43 (2-3): 199-203.

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