Spectrin antibody | AA6 (7A3)

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Mouse anti Chicken Spectrin (Non-Erythroid)

Product Type
Monoclonal Antibody
Clone
AA6 (7A3)
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
8365-1256 Ch IF IP WB 50 µg
Mouse anti Chicken spectrin, clone AA6 recognizes spectrin alpha chain, non-erythrocytic 1, also known as Alpha-II spectrin and Fodrin alpha chain, encoded by the SPTAN1 gene. Mouse anti chicken spectrin recognizes both chicken spectrin and the alpha chain of non-erythroid spectrin in mammalian cells.

Mouse anti Chicken spectrin, clone AA6 reacts with the 240 kDa full length spectrin alpha, the ~150 kDa, 145 kDa characteristic breakdown products of calpain and caspase-3 (Nath et al. 1996) and the ~120 kDa product, a result of caspase-3 activation (Nath et al. 1998).

Product Details

Target Species
Chicken
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rabbit
Rat
Guinea Pig
Human
Mouse
Pig
Mammals Expected from Sequence
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Chicken blood cell membrane purified by hypotonic lysis and mechanical enucleation.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P07751 Related reagents
Entrez Gene
SPTAN1 Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0003779 actin binding
GO:0005516 calmodulin binding
GO:0005856 cytoskeleton
GO:0005938 cell cortex
GO:0051693 actin filament capping
Regulatory
For research purposes only

Applications of Spectrin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Chromatin Immunoprecipitation
Immunofluorescence 1/200 1/400
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Clone AA6 recognizes the 150kDa and 120kDa spectrin cleavage products in staurosporine-treated HeLa lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for Spectrin antibody

  1. Ylikoski, J. et al. (1992) Distribution of F-actin and fodrin in the hair cells of the guinea pig cochlea as revealed by confocal fluorescence microscopy.
    Hear Res. 60 (1): 80-8.
  2. Ylikoski, J. et al. (1990) Nonerythroid spectrin (fodrin) is a prominent component of the cochlear hair cells.
    Hear Res. 43 (2-3): 199-203.