IL-6 antibody | AV129

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Mouse anti Chicken Interleukin-6:HRP

Product Type
Monoclonal Antibody
Clone
AV129
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5932P E WB 0.1 mg
Mouse anti Chicken Interleukin-6 antibody, clone AV129 recognizes chicken interleukin-6 (IL-6), a cytokine that can function in either a pro-inflammatory or in an anti-inflammatory capacity.

Chicken interleukin-6 is produced by a range of cell types including macrophages, endothelial cells, T-cells and fibroblasts. IL-6 is a multipotential cytokine with the ability to induce proliferation and differentiation in B-cells and T-cells and acts as an important mediator in acute phase response.

This antibody was raised as part of the UK BBSRC funded avian immunology toolbox project and is one of a number reagents now available from Bio-Rad developed from this project.

Product Details

Target Species
Chicken
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
HRP Stabiliser (BUF052A)
Immunogen
Chicken interleukin-6 (IL-6)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the SP2/0 myeloma cell line

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch

More Information

UniProt
Q90YI0 Related reagents
Entrez Gene
IL6 Related reagents
GO Terms
GO:0006953 acute-phase response
GO:0005125 cytokine activity
GO:0005138 interleukin-6 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
Regulatory
For research purposes only

Applications of IL-6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/20,000 1/200,000
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Recombinant Chicken Interleukin-6 PAP003 50 µg FN IA