Beta 2 Microglobulin antibody | F21-21
Mouse anti Chicken β2 microglobulin antibody, clone F21-21 recognises chicken β2 microglobulin, a component of MHC class I molecules and is expressed on nearly all nucleated cells.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Turkey
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
- Buffer Solution
- Borate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- P21611 Related reagents
- Entrez Gene
- B2M Related reagents
- GO Terms
- GO:0002474 antigen processing and presentation of peptide antigen via MHC class I
- GO:0042612 MHC class I protein complex
- GO:0005576 extracellular region
- GO:0006955 immune response
- For research purposes only
Applications of Beta 2 Microglobulin antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)||STAR117F||0.5 mg||F|
|Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)||STAR117P||0.5 mg||E WB|
|Rabbit F(ab')2 anti Mouse IgG:RPE||STAR12A||1 ml||F|
|Rabbit F(ab')2 anti Mouse IgG:FITC||STAR9B||1 mg||F|
Product Specific References
References for Beta 2 Microglobulin antibody
Pinard, M.H. & Hepkema, B.G. (1993) Biochemical and serological identification of major histocompatibility complex antigens in outbred chickens.
Vet Immunol Immunopathol. 39 (4): 407-19.
Levy, A.M. et al. (2003) Major histocompatibility complex class I is downregulated in Marek's disease virus infected chicken embryo fibroblasts and corrected by chicken interferon.
Comp Immunol Microbiol Infect Dis. 26 (3): 189-98.
Dunon, D. et al. (1990) T cell precursor migration towards beta 2-microglobulin is involved in thymus colonization of chicken embryos.
EMBO J. 9 (10): 3315-22.
Juul-Madsen, H.R. et al. (2004) Influence of early or late start of first feeding on growth and immune phenotype of broilers.
Br Poult Sci. 45 (2): 210-22.
Skjødt, K. et al. (1986) Isolation and characterization of chicken and turkey beta 2-microglobulin.
Mol Immunol. 23 (12): 1301-9.
Pickel JM et al. (1990) An avian B-lymphocyte protein associated with beta 2-microglobulin.
Immunogenetics. 32 (1): 1-7.
Buitenhuis, A.J. et al. (2006) Altered circulating levels of serotonin and immunological changes in laying hens divergently selected for feather pecking behavior.
Poult Sci. 85 (10): 1722-8.
Juul-Madsen, H.R. et al. (2002) Major histocompatibility complex-linked immune response of young chickens vaccinated with an attenuated live infectious bursal disease virus vaccine followed by an infection.
Poult Sci. 81 (5): 649-56.
Juul-Madsen, H.R. et al. (2006) Immune response to a killed infectious bursal disease virus vaccine in inbred chicken lines with different major histocompatibility complex haplotypes.
Poult Sci. 85 (6): 986-98.
Butter, C. et al. (2013) The peptide motif of the single dominantly expressed class I molecule of the chicken MHC can explain the response to a molecular defined vaccine of infectious bursal disease virus (IBDV).
Immunogenetics. 65 (8): 609-18.
Juul-Madsen, H.R. et al. (2000) Molecular characterization of major and minor MHC class I and II genes in B21-like haplotypes in chickens.
Anim Genet. 31 (4): 252-61.
Møller, L.B. et al. (1991) Variations in the cytoplasmic region account for the heterogeneity of the chicken MHC class I (B-F) molecules.
Immunogenetics. 34 (2): 110-20.
Wallny, H.J. et al. (2006) Peptide motifs of the single dominantly expressed class I molecule explain the striking MHC-determined response to Rous sarcoma virus in chickens.
Proc Natl Acad Sci U S A. 103 (5): 1434-9.
Walker, B.A. et al. (2011) The dominantly expressed class I molecule of the chicken MHC is explained by coevolution with the polymorphic peptide transporter (TAP) genes.
Proc Natl Acad Sci U S A. 108 (20): 8396-401.
Hepkema, B.G. et al. (1991) Biochemical identification of B-F and B-G allelic variants of the chicken major histocompatibility complex.
Anim Genet. 22 (4): 323-32.
Burgess, S.C. & Davison, T.F. (1999) Counting absolute numbers of specific leukocyte subpopulations in avian whole blood using a single-step flow cytometric technique: comparison of two inbred lines of chickens.
J Immunol Methods. 227 (1-2): 169-76.
Lawson S et al. (2001) Turkey and chicken interferon-gamma, which share high sequence identity, are biologically cross-reactive.
Dev Comp Immunol. 25 (1): 69-82.