Beta 2 Microglobulin antibody | F21-21

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Mouse anti Chicken Beta 2 Microglobulin

Product Type
Monoclonal Antibody
Clone
F21-21
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5774 C F IP WB 0.25 mg
Mouse anti Chicken β2 microglobulin antibody, clone F21-21 recognises chicken β2 microglobulin, a component of MHC class I molecules and is expressed on nearly all nucleated cells.

Product Details

Target Species
Chicken
Species Cross-Reactivity
Target SpeciesCross Reactivity
Turkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Borate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P21611 Related reagents
Entrez Gene
B2M Related reagents
GO Terms
GO:0002474 antigen processing and presentation of peptide antigen via MHC class I
GO:0042612 MHC class I protein complex
GO:0005576 extracellular region
GO:0006955 immune response
Regulatory
For research purposes only

Applications of Beta 2 Microglobulin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for Beta 2 Microglobulin antibody

  1. Pinard, M.H. & Hepkema, B.G. (1993) Biochemical and serological identification of major histocompatibility complex antigens in outbred chickens.
    Vet Immunol Immunopathol. 39 (4): 407-19.
  2. Levy, A.M. et al. (2003) Major histocompatibility complex class I is downregulated in Marek's disease virus infected chicken embryo fibroblasts and corrected by chicken interferon.
    Comp Immunol Microbiol Infect Dis. 26 (3): 189-98.
  3. Dunon, D. et al. (1990) T cell precursor migration towards beta 2-microglobulin is involved in thymus colonization of chicken embryos.
    EMBO J. 9 (10): 3315-22.
  4. Juul-Madsen, H.R. et al. (2004) Influence of early or late start of first feeding on growth and immune phenotype of broilers.
    Br Poult Sci. 45 (2): 210-22.
  5. Skjødt, K. et al. (1986) Isolation and characterization of chicken and turkey beta 2-microglobulin.
    Mol Immunol. 23 (12): 1301-9.
  6. Pickel JM et al. (1990) An avian B-lymphocyte protein associated with beta 2-microglobulin.
    Immunogenetics. 32 (1): 1-7.
  7. Buitenhuis, A.J. et al. (2006) Altered circulating levels of serotonin and immunological changes in laying hens divergently selected for feather pecking behavior.
    Poult Sci. 85 (10): 1722-8.
  8. Juul-Madsen, H.R. et al. (2002) Major histocompatibility complex-linked immune response of young chickens vaccinated with an attenuated live infectious bursal disease virus vaccine followed by an infection.
    Poult Sci. 81 (5): 649-56.
  9. Juul-Madsen, H.R. et al. (2006) Immune response to a killed infectious bursal disease virus vaccine in inbred chicken lines with different major histocompatibility complex haplotypes.
    Poult Sci. 85 (6): 986-98.
  10. Butter, C. et al. (2013) The peptide motif of the single dominantly expressed class I molecule of the chicken MHC can explain the response to a molecular defined vaccine of infectious bursal disease virus (IBDV).
    Immunogenetics. 65 (8): 609-18.
  11. Juul-Madsen, H.R. et al. (2000) Molecular characterization of major and minor MHC class I and II genes in B21-like haplotypes in chickens.
    Anim Genet. 31 (4): 252-61.
  12. Møller, L.B. et al. (1991) Variations in the cytoplasmic region account for the heterogeneity of the chicken MHC class I (B-F) molecules.
    Immunogenetics. 34 (2): 110-20.
  13. Wallny, H.J. et al. (2006) Peptide motifs of the single dominantly expressed class I molecule explain the striking MHC-determined response to Rous sarcoma virus in chickens.
    Proc Natl Acad Sci U S A. 103 (5): 1434-9.
  14. Walker, B.A. et al. (2011) The dominantly expressed class I molecule of the chicken MHC is explained by coevolution with the polymorphic peptide transporter (TAP) genes.
    Proc Natl Acad Sci U S A. 108 (20): 8396-401.
  15. Hepkema, B.G. et al. (1991) Biochemical identification of B-F and B-G allelic variants of the chicken major histocompatibility complex.
    Anim Genet. 22 (4): 323-32.
  16. Burgess, S.C. & Davison, T.F. (1999) Counting absolute numbers of specific leukocyte subpopulations in avian whole blood using a single-step flow cytometric technique: comparison of two inbred lines of chickens.
    J Immunol Methods. 227 (1-2): 169-76.
  17. Lawson S et al. (2001) Turkey and chicken interferon-gamma, which share high sequence identity, are biologically cross-reactive.
    Dev Comp Immunol. 25 (1): 69-82.