CCT Theta antibody | PK/13/72/8k

Rat anti CCT Theta

Product Type
Monoclonal Antibody
Clone
PK/13/72/8k
Isotype
IgG2a
Specificity
CCT Theta

Product Code Applications Pack Size List Price Your Price Qty
MCA2180
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IP WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti CCT theta antibody, clone PK/13/72/8k recognizes the theta polypeptide of the CCT chaperonin molecule complex, also known as T-complex protein 1 subunit theta, CCT8 or CCTθ, a 547 amino acid ~:60 kDa molecular chaperone.

The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumor suppressor protein.

Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rabbit
Human
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/250 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA2180 detects a band of approximately 60kD in lysates of heat shocked Hela cells.

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References for CCT Theta antibody

  1. Hynes, G. et al. (1996) Analysis of chaperonin-containing TCP-1 subunits in the human keratinocyte two-dimensional protein database: further characterisation of antibodies to individual subunits.
    Electrophoresis. 17 (11): 1720-7.
  2. Llorca, O. et al. (2000) Eukaryotic chaperonin CCT stabilizes actin and tubulin folding intermediates in open quasi-native conformations.
    EMBO J. 19 (22): 5971-9.
  3. Hynes, G.M. & Willison, K.R. (2000) Individual subunits of the eukaryotic cytosolic chaperonin mediate interactions with binding sites located on subdomains of beta-actin.
    J Biol Chem. 275 (25): 18985-94.
  4. Plimpton, R.L. et al. (2015) Structures of the Gβ-CCT and PhLP1-G&beta-CCT complexes reveal a mechanism for G-protein β-subunit folding and Gβγγ dimer assembly.
    Proc Natl Acad Sci U S A. 112 (8): 2413-8.
  5. Slater, L.H. et al. (2013) CCT chaperonin complex is required for efficient delivery of anthrax toxin into the cytosol of host cells.
    Proc Natl Acad Sci U S A. 110 (24): 9932-7.

RRID
AB_2228910
UniProt
Q3ZCI9
Entrez Gene
CCT8
GO Terms
GO:0051082 unfolded protein binding
GO:0006457 protein folding
GO:0005524 ATP binding
GO:0005815 microtubule organizing center
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"