Staphylococcus aureus antibody | Staph11-232.3
Mouse anti Staphylococcus aureus antibody, clone Staph11-232.3 (MCA5792) used as a capture reagent in an ELISA to evaluate linear peptide binding.
Image caption:
Both SP27 and MAP27' bind to anti-PGN mAb specifically. (A) The sequences of SP27 (Biotin-SASPHHHSRLRSESGG) and SP27' (Biotin-SPHHHSRLRSESSAGG). Underlined letters represent nonspecific flanking amino acids. (B) Both SP27 and SP27' bind to anti-PGN mAb in a dose-dependent manner. (C) Both SP27 and SP27' (100 μg/ml) specifically bind to anti-PGN mAb, but not to anti-LTA mAb. For ELISA assays in panel B and C, anti-PGN mAb or anti-LTA mAb was used to coat the wells at a concentration of 5 μg/ml, SP27, SP27' or non-specific peptide L2 (in panel B, sequence: Biotin-HSGHWDFRQWWQPSGG) was then added at indicated concentrations and incubated at 37°C for 40 min, followed by detection with HRP-labeled streptavidin. (D) The structure diagram of MAP27. MAP27 was synthesized in a tetra-branched form that contains four copies of a sequence (SASPHHHSRLRSESGG) that mimics PGN epitope. (E) MAP27 binds to anti-PGN mAb specifically. (F) MAP27 binds to anti-S. aureus polyclonal antibodies specifically. For ELISA assays in panel E and F, MAP27 or MAPctrl was used to coat the wells of a microplate. Anti-PGN mAb or anti-S. aureus polyclonal antibodies was added, followed by detection with HRP-labeled antibodies. The absorbance was measured at OD450nm. The results are shown as means ±SEM. * P<0.05, ** P<0.01, *** P<0.001.
From: Wang X-Y, Huang Z-X, Chen Y-G, Lu X, Zhu P, Wen K, et al. (2015)
A Multiple Antigenic Peptide Mimicking Peptidoglycan Induced T Cell Responses to Protect Mice from Systemic Infection with Staphylococcus aureus.
PLoS ONE 10(8): e0136888.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Staphylococcus aureus ATCC 29740 antibody, clone Staph11-232.3 recognizes the peptidoglycan of S. aureus, protein A-negative S. aureus and S. epidermidis.
Mouse anti Staphylococcus aureus ATCC 29740 antibody, clone Staph11-232.3 does not cross-react with Streptococcus sp., Mycoplasma sp. or Cornybacteria sp., however it has not been cross-adsorbed and may react with peptidoglycans from other related species.
Mouse anti Staphylococcus aureus ATCC 29740 antibody, clone Staph11-232.3 does not cross-react with Streptococcus sp., Mycoplasma sp. or Cornybacteria sp., however it has not been cross-adsorbed and may react with peptidoglycans from other related species.
Product Details
- Target Species
- Bacterial
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG3 prepared by PEG precipitation from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- UV-inactivated Staphylococcus aureus cells (ATCC 29740).
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of Staphylococcus aureus antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/1000 | 1/5000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Staphylococcus aureus antibody
-
Chen, Y. et al. (2011) Peptide mimics of peptidoglycan are vaccine candidates and protect mice from infection with Staphylococcus aureus.
J Med Microbiol. 60: 995-1002. -
Wang, X.Y. et al. (2015) A Multiple Antigenic Peptide Mimicking Peptidoglycan Induced T Cell Responses to Protect Mice from Systemic Infection with Staphylococcus aureus.
PLoS One. 10 (8): e0136888.
Further Reading
-
Su, F.Y. et al. (2007) Simple and sensitive bacterial quantification by a flow-based kinetic exclusion fluorescence immunoassay.
Biosens Bioelectron. 22: 2500-7.
