Staphylococcus aureus antibody | Staph11-232.3

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Anti Staphylococcus aureus ATCC 29740 Antibody, clone Staph11-232.3 gallery image 1 — **Published customer image:**
Mouse anti *Staphylococcus aureus* antibody, clone Staph11-232.3 (**MCA5792**) used as a capture reagent in an ELISA to evaluate linear peptide binding.
**Image caption:**
Both SP27 and MAP27' bind to anti-PGN mAb specifically. (A) The sequences of SP27 (Biotin-SASPHHHSRLRSESGG) and SP27' (Biotin-SPHHHSRLRSESSAGG). Underlined letters represent nonspecific flanking amino acids. (B) Both SP27 and SP27' bind to anti-PGN mAb in a dose-dependent manner. (C) Both SP27 and SP27' (100 μg/ml) specifically bind to anti-PGN mAb, but not to anti-LTA mAb. For ELISA assays in panel B and C, anti-PGN mAb or anti-LTA mAb was used to coat the wells at a concentration of 5 μg/ml, SP27, SP27' or non-specific peptide L2 (in panel B, sequence: Biotin-HSGHWDFRQWWQPSGG) was then added at indicated concentrations and incubated at 37°C for 40 min, followed by detection with HRP-labeled streptavidin. (D) The structure diagram of MAP27. MAP27 was synthesized in a tetra-branched form that contains four copies of a sequence (SASPHHHSRLRSESGG) that mimics PGN epitope. (E) MAP27 binds to anti-PGN mAb specifically. (F) MAP27 binds to anti-*S. aureus* polyclonal antibodies specifically. For ELISA assays in panel E and F, MAP27 or MAPctrl was used to coat the wells of a microplate. Anti-PGN mAb or anti-*S. aureus* polyclonal antibodies was added, followed by detection with HRP-labeled antibodies. The absorbance was measured at OD450nm. The results are shown as means ±SEM. * P<0.05, ** P<0.01, *** P<0.001.

From: Wang X-Y, Huang Z-X, Chen Y-G, Lu X, Zhu P, Wen K, *et al*. (2015)
A Multiple Antigenic Peptide Mimicking Peptidoglycan Induced T Cell Responses to Protect Mice from Systemic Infection with *Staphylococcus aureus*.
PLoS ONE 10(8): e0136888.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Staphylococcus aureus ATCC 29740

Product Type: Monoclonal Antibody
Clone: Staph11-232.3
Isotype: IgG3

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA5792	E			0.2 mg

Mouse anti Staphylococcus aureus ATCC 29740 antibody, clone Staph11-232.3 recognizes the peptidoglycan of S. aureus, protein A-negative S. aureus and S. epidermidis.

Mouse anti Staphylococcus aureus ATCC 29740 antibody, clone Staph11-232.3 does not cross-react with Streptococcus sp., Mycoplasma sp. or Cornybacteria sp., however it has not been cross-adsorbed and may react with peptidoglycans from other related species.

Product Details

Target Species: Bacterial
Product Form: Purified IgG - liquid
Preparation: Purified IgG3 prepared by PEG precipitation from ascites
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.1% Sodium Azide (NaN₃)
Immunogen: UV-inactivated Staphylococcus aureus cells (ATCC 29740).
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.

More Information

Regulatory: For research purposes only

Applications of Staphylococcus aureus antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1/1000	1/5000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG3:FITC	HCA039F	0.1 mg	F
Human anti Mouse IgG3:HRP	HCA039P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for Staphylococcus aureus antibody

Chen, Y. et al. (2011) Peptide mimics of peptidoglycan are vaccine candidates and protect mice from infection with Staphylococcus aureus.
J Med Microbiol. 60: 995-1002.
Wang, X.Y. et al. (2015) A Multiple Antigenic Peptide Mimicking Peptidoglycan Induced T Cell Responses to Protect Mice from Systemic Infection with Staphylococcus aureus.
PLoS One. 10 (8): e0136888.