Salmonella antibody | 5D12A

100% Secure

No associated image currently available

Mouse anti Salmonella Broad Reactivity

Product Type: Monoclonal Antibody
Clone: 5D12A
Isotype: IgG1

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
MCA2832 Datasheet	E IB IF SDS	0.2 mg	Log in		Get Quote

Mouse anti Salmonella Broad Reactivity antibody, clone 5D12A recognizes the core antigen of Salmonella enterica, serogroups A, B, C₁, C₂, D, E₁ and E₂. The core antigen bears the O antigen and is attached to lipid A. These three components together form the lipopolysaccharide (LPS), a major component of the outer membrane of Gram-negative bacteria. The LPS is an endotoxin and induces a strong immune response from the host. Its mutation or removal results in the death of the bacterial cell and as such is a prime target for antimicrobial drugs.

Salmonella enterica,causes typhoid fever, paratyphoid fever and foodborne illnesses.

Mouse anti Salmonella Broad Reactivity antibody, clone 5D12A cross-reacts with serogroup A (S. paratyphi A), serogroup B (S. typhimurium), serogroup C₁ (S. choleraesuis.), serogroup C₂, (S. newport), serogroup D (S. enteriditis), serogroup E₁ (S. anatum) and serogroup E₂ (S. selandia). It does not cross-react with Escherichia coli 055:B5, E. coli K12 or Klebsiella pneumoniae.

Product Details

Target Species: Bacterial
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: <0.1% Sodium Azide (NaN₃)
Immunogen: Heat inactivated Salmonella paratyphi.
Approx. Protein Concentrations: IgG concentration 1.0mg/ml
Fusion Partners: Spleen cells from immunized Balb/c mice were fused with cells of the NS0/1, a subclone of the P3X63Ag8 myeloma cell line.

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

Regulatory: For research purposes only

Applications of Salmonella antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Immunoblotting
Immunofluorescence

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropritate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	E WB	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	F IF	0.1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)	STAR117D550	F IF WB	0.1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)	STAR117D650	F IF	0.1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	F WB	0.1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	F IF WB	0.1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	F	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	C E WB	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG (Fc):FITC	STAR120F	C F	1 mg	Log in	Get Quote
Goat anti Mouse IgG (Fc):HRP	STAR120P	E WB	1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	F	1 ml	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	C E P RE WB	1 mg	Log in	Get Quote
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	F	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	F	1 ml	Log in	Get Quote
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	C E P	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	C E WB	1 mg	Log in	Get Quote
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	E	1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	F IF WB	0.1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	F	1 mg	Log in	Get Quote

Application Based External Images

Immunofluorescence

Product Specific References

Source Reference

Merkulova TI et al. (1995) Monoclonal antibodies directed against unique and common determinants on the lipopolysaccharide molecule of Salmonella serogroups A, B, and D.
Hybridoma. 14 (6): 557-62.

Antibody Characterization Reference

Savers, S. (1995) MAb SA:5D12A, anti-Salmonella LPS
Hybridoma. April 2009, 14(6): 619-619.

References for Salmonella antibody

Marinis, J.M. et al. (2011) A Novel Motif in the Crohn's Disease Susceptibility Protein, NOD2, Allows TRAF4 to Down-regulate Innate Immune Responses.
J Biol Chem. 286: 1938-50.
Lomakina G. Yu. et al. (2014) Synthesis and application of firefly luciferase antibody conjugates in a bioluminescent immunoassay of Salmonella cells
Moscow University Chemistry Bulletin. 69 (2): 49-55.
Cruz-Adalia, A. et al. (2014) T cells kill bacteria captured by transinfection from dendritic cells and confer protection in mice.
Cell Host Microbe. 15 (5): 611-22.
Hesse, M. et al. (2016) Immune response of turkey poults exposed at 1 day of age to either attenuated or wild Salmonella strains.
Vet Immunol Immunopathol. 174: 1-10.
Braukmann, M. et al. (2016) Combination of competitive exclusion and immunisation with a live Salmonella vaccine in newly hatched chickens: Immunological and microbiological effects.
Res Vet Sci. 107: 34-41.

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸

Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra