Salmonella antibody | 5D12A
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Mouse anti Salmonella Broad Reactivity
- Product Type
- Monoclonal Antibody
- Clone
- 5D12A
- Isotype
- IgG1
- Specificity
- Salmonella
- Region
- BROAD REACTIVITY
Antibody Quality and 
Unrivaled 
| Mouse anti Salmonella Broad Reactivity antibody, clone 5D12A recognizes the core antigen of Salmonella enterica, serogroups A, B, C1, C2, D, E1 and E2. The core antigen bears the O antigen and is attached to lipid A. These three components together form the lipopolysaccharide (LPS), a major component of the outer membrane of Gram-negative bacteria. The LPS is an endotoxin and induces a strong immune response from the host. Its mutation or removal results in the death of the bacterial cell and as such is a prime target for antimicrobial drugs. Salmonella enterica,causes typhoid fever, paratyphoid fever and foodborne illnesses. Mouse anti Salmonella Broad Reactivity antibody, clone 5D12A cross-reacts with serogroup A (S. paratyphi A), serogroup B (S. typhimurium), serogroup C1 (S. choleraesuis.), serogroup C2, (S. newport), serogroup D (S. enteriditis), serogroup E1 (S. anatum) and serogroup E2 (S. selandia). It does not cross-react with Escherichia coli 055:B5, E. coli K12 or Klebsiella pneumoniae. |
- Target Species
- Bacterial
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Heat inactivated Salmonella paratyphi.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the NS0/1, a subclone of the P3X63Ag8 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunoblotting | |
||
| Immunofluorescence | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropritate negative/positive controls.
Source Reference
-
Merkulova TI et al. (1995) Monoclonal antibodies directed against unique and common determinants on the lipopolysaccharide molecule of Salmonella serogroups A, B, and D.
Hybridoma. 14 (6): 557-62.
Antibody Characterization Reference
-
Savers, S. (1995) MAb SA:5D12A, anti-Salmonella LPS
Hybridoma. April 2009, 14(6): 619-619.
References for Salmonella antibody
-
Marinis, J.M. et al. (2011) A Novel Motif in the Crohn's Disease Susceptibility Protein, NOD2, Allows TRAF4 to Down-regulate Innate Immune Responses.
J Biol Chem. 286: 1938-50. -
Lomakina G. Yu. et al. (2014) Synthesis and application of firefly luciferase antibody conjugates in a bioluminescent immunoassay of Salmonella cells
Moscow University Chemistry Bulletin. 69 (2): 49-55. -
Cruz-Adalia, A. et al. (2014) T cells kill bacteria captured by transinfection from dendritic cells and confer protection in mice.
Cell Host Microbe. 15 (5): 611-22. -
Hesse, M. et al. (2016) Immune response of turkey poults exposed at 1 day of age to either attenuated or wild Salmonella strains.
Vet Immunol Immunopathol. 174: 1-10. -
Braukmann, M. et al. (2016) Combination of competitive exclusion and immunisation with a live Salmonella vaccine in newly hatched chickens: Immunological and microbiological effects.
Res Vet Sci. 107: 34-41.
- RRID
- AB_1125358
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