Salmonella antibody | 5D12A

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Mouse anti Salmonella Broad Reactivity

Product Type
Monoclonal Antibody
Clone
5D12A
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2832 E IB IF 0.2 mg
Mouse anti Salmonella Broad Reactivity antibody, clone 5D12A recognizes the core antigen of Salmonella enterica, serogroups A, B, C1, C2, D, E1 and E2. The core antigen bears the O antigen and is attached to lipid A. These three components together form the lipopolysaccharide (LPS), a major component of the outer membrane of Gram-negative bacteria. The LPS is an endotoxin and induces a strong immune response from the host. Its mutation or removal results in the death of the bacterial cell and as such is a prime target for antimicrobial drugs.

Salmonella enterica,causes typhoid fever, paratyphoid fever and foodborne illnesses.

Mouse anti Salmonella Broad Reactivity antibody, clone 5D12A cross-reacts with serogroup A (S. paratyphi A), serogroup B (S. typhimurium), serogroup C1 (S. choleraesuis.), serogroup C2, (S. newport), serogroup D (S. enteriditis), serogroup E1 (S. anatum) and serogroup E2 (S. selandia). It does not cross-react with Escherichia coli 055:B5, E. coli K12 or Klebsiella pneumoniae.

Product Details

Target Species
Bacterial
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Heat inactivated Salmonella paratyphi.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the NS0/1, a subclone of the P3X63Ag8 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Salmonella antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunoblotting
Immunofluorescence
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropritate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Immunofluorescence

Product Specific References

Source Reference

  1. Merkulova TI et al. (1995) Monoclonal antibodies directed against unique and common determinants on the lipopolysaccharide molecule of Salmonella serogroups A, B, and D.
    Hybridoma. 14 (6): 557-62.

Antibody Characterization Reference

  1. Savers, S. (1995) MAb SA:5D12A, anti-Salmonella LPS
    Hybridoma. April 2009, 14(6): 619-619.

References for Salmonella antibody

  1. Marinis, J.M. et al. (2011) A Novel Motif in the Crohn's Disease Susceptibility Protein, NOD2, Allows TRAF4 to Down-regulate Innate Immune Responses.
    J Biol Chem. 286: 1938-50.
  2. Lomakina G. Yu. et al. (2014) Synthesis and application of firefly luciferase antibody conjugates in a bioluminescent immunoassay of Salmonella cells
    Moscow University Chemistry Bulletin. 69 (2): 49-55.
  3. Cruz-Adalia, A. et al. (2014) T cells kill bacteria captured by transinfection from dendritic cells and confer protection in mice.
    Cell Host Microbe. 15 (5): 611-22.
  4. Hesse, M. et al. (2016) Immune response of turkey poults exposed at 1 day of age to either attenuated or wild Salmonella strains.
    Vet Immunol Immunopathol. 174: 1-10.
  5. Braukmann, M. et al. (2016) Combination of competitive exclusion and immunisation with a live Salmonella vaccine in newly hatched chickens: Immunological and microbiological effects.
    Res Vet Sci. 107: 34-41.