Salmonella antibody | 5D12A

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Mouse anti Salmonella Broad Reactivity

Product Type: Monoclonal Antibody
Clone: 5D12A
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2832	E IB IF			0.2 mg

Mouse anti Salmonella Broad Reactivity antibody, clone 5D12A recognizes the core antigen of Salmonella enterica, serogroups A, B, C₁, C₂, D, E₁ and E₂. The core antigen bears the O antigen and is attached to lipid A. These three components together form the lipopolysaccharide (LPS), a major component of the outer membrane of Gram-negative bacteria. The LPS is an endotoxin and induces a strong immune response from the host. Its mutation or removal results in the death of the bacterial cell and as such is a prime target for antimicrobial drugs.

Salmonella enterica,causes typhoid fever, paratyphoid fever and foodborne illnesses.

Mouse anti Salmonella Broad Reactivity antibody, clone 5D12A cross-reacts with serogroup A (S. paratyphi A), serogroup B (S. typhimurium), serogroup C₁ (S. choleraesuis.), serogroup C₂, (S. newport), serogroup D (S. enteriditis), serogroup E₁ (S. anatum) and serogroup E₂ (S. selandia). It does not cross-react with Escherichia coli 055:B5, E. coli K12 or Klebsiella pneumoniae.

Product Details

Target Species

Bacterial

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Immunogen

Heat inactivated Salmonella paratyphi.

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Fusion Partners

Spleen cells from immunized Balb/c mice were fused with cells of the NS0/1, a subclone of the P3X63Ag8 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

Regulatory: For research purposes only

Applications of Salmonella antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Immunoblotting
Immunofluorescence

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropritate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG1:HRP	HCA036P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Application Based External Images

Immunofluorescence

Product Specific References

Source Reference

Merkulova TI et al. (1995) Monoclonal antibodies directed against unique and common determinants on the lipopolysaccharide molecule of Salmonella serogroups A, B, and D.
Hybridoma. 14 (6): 557-62.

Antibody Characterization Reference

Savers, S. (1995) MAb SA:5D12A, anti-Salmonella LPS
Hybridoma. April 2009, 14(6): 619-619.

References for Salmonella antibody

Marinis, J.M. et al. (2011) A Novel Motif in the Crohn's Disease Susceptibility Protein, NOD2, Allows TRAF4 to Down-regulate Innate Immune Responses.
J Biol Chem. 286: 1938-50.
Lomakina G. Yu. et al. (2014) Synthesis and application of firefly luciferase antibody conjugates in a bioluminescent immunoassay of Salmonella cells
Moscow University Chemistry Bulletin. 69 (2): 49-55.
Cruz-Adalia, A. et al. (2014) T cells kill bacteria captured by transinfection from dendritic cells and confer protection in mice.
Cell Host Microbe. 15 (5): 611-22.
Hesse, M. et al. (2016) Immune response of turkey poults exposed at 1 day of age to either attenuated or wild Salmonella strains.
Vet Immunol Immunopathol. 174: 1-10.
Braukmann, M. et al. (2016) Combination of competitive exclusion and immunisation with a live Salmonella vaccine in newly hatched chickens: Immunological and microbiological effects.
Res Vet Sci. 107: 34-41.

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